Results 11 to 20 of about 733,470 (308)

Cationic lipid-mediated intracellular delivery of antibodies into live cells

BioTechniques, 2008
The ability to introduce antibodies to live cells opens new insights to a wide range of applications, such as protein intracellular trafficking studies, protein interference studies with blocking antibodies, and live immunolabeling or protein ...
Claire O. Weill, Stéphanie Biri, Patrick Erbacher   +2 more
doaj   +1 more source

Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate

Pharmaceutics, 2022
Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport.
Tina Vindiš, Anja Blažič, Diaa Khayyat, Tjaša Potočnik, Shaurya Sachdev, Lea Rems   +5 more
doaj   +1 more source

Inhibition of Sox2 Expression in the Adult Neural Stem Cell Niche In Vivo by Monocationic-based siRNA Delivery

Molecular Therapy: Nucleic Acids, 2013
RNA interference (RNAi) is a major tool for basic and applied investigations. However, obtaining RNAi data that have physiological significance requires investigation of regulations and therapeutic strategies in appropriate in vivo settings.
Sylvie Remaud, Silvia Alejandra López-Juárez, Anne-Laure Bolcato-Bellemin, Patrick Neuberg, Fabrice Stock, Marie-Elise Bonnet, Rym Ghaddab, Marie Stéphanie Clerget-Froidevaux, Jacqueline Pierre-Simons, Patrick Erbacher, Barbara A Demeneix, Ghislaine Morvan-Dubois   +11 more
doaj   +1 more source

Infrared Laser-Based Single Cell Permeabilization by Plasma Membrane Temperature Gradients

Membranes, 2022
Single cell microinjection provides precise tuning of the volume and timing of delivery into the treated cells; however, it also introduces workflow complexity that requires highly skilled operators and specialized equipment.
Allen L. Garner, Bogdan Neculaes, Dmitry V. Dylov   +2 more
doaj   +1 more source

Study on the Mutation Sites of CSF3R Responsible for the Clonal Advantage of Chronic Neutrophilic Leukemia

Central Asian Journal of Medical Sciences, 2016
Objectives: Chronic neutrophilic leukemia (CNL) is a rare, but distinct, entity within myeloproliferative neoplasms, characterized by splenomegaly, sustained mature neutrophilia, and absence of the Philadelphia chromosome. In 2013, oncogenic mutations in
Otgonbat Altangerel, Ming Feng Zhao, Wu Ri Mao   +2 more
doaj   +1 more source

WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers [PDF]

Iranian Journal of Basic Medical Sciences, 2019
Objective(s): Breast cancer is the second leading cause of cancer death in females. Understanding molecular mechanisms in cancer cells compared with normal cells is crucial for diagnostic and therapeutic strategies. Long intergenic non-protein coding RNA,
Sara Kor, vahid Erfani-Moghadam, Reza Sahebi, Shabbou Bahramian, Mohammad Shafiee   +4 more
doaj   +1 more source

Transfection by Electroporation of Cancer and Primary Cells Using Nanosecond and Microsecond Electric Fields

Pharmaceutics, 2022
Gene transfer into primary immune cells as well as into cell lines is essential for scientific and therapeutical applications. One of the methods used for gene transfer is electroporation (EP).
Eivina Radzevičiūtė, Veronika Malyško-Ptašinskė, Jurij Novickij, Vitalij Novickij, Irutė Girkontaitė   +4 more
doaj   +1 more source

Second-Generation Polyamidoamine Dendrimer Conjugated with Oligopeptides Can Enhance Plasmid DNA Delivery In Vitro

Molecules, 2023
Poly(amidoamine) (PAMAM) dendrimers have attracted considerable attention in the field of gene therapy due to their flexibility in introducing different functional moieties and reduced toxicity at low generations.
Seongyeon Kim, Le Thi Thuy, Jeil Lee, Joon Sig Choi   +3 more
doaj   +1 more source

Genome-Wide CRISPR-Cas9 Screen Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection

mSphere, 2020
Double-stranded RNA (dsRNA) is the hallmark of many viral infections. dsRNA is produced either by RNA viruses during replication or by DNA viruses upon convergent transcription.
Olivier Petitjean, Erika Girardi, Richard Patryk Ngondo, Vladimir Lupashin, Sébastien Pfeffer   +4 more
doaj   +1 more source

In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint

Biomedicines, 2021
Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics.
Lisa Sturm, Bettina Schwemberger, Ursula Menzel, Sonja Häckel, Christoph E. Albers, Christian Plank, Jaap Rip, Mauro Alini, Andreas Traweger, Sibylle Grad, Valentina Basoli   +10 more
doaj   +1 more source