Results 11 to 20 of about 971,477 (354)
A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
Theranostics, 2020 Gene editing is a versatile technique in biomedicine that promotes fundamental research as well as clinical therapy. The development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) as a genome editing machinery has accelerated the ...
A. K. Fajrial +4 more
semanticscholar +1 more source
Infectious viruses from transfected SARS-CoV-2 genomic RNA
Frontiers in Bioengineering and Biotechnology, 2023 SARS-CoV-2 emerged at the end of 2019, and like other novel pathogens causing severe symptoms, WHO recommended heightened biosafety measures for laboratories working with the virus.
Elina Väisänen +7 more
doaj +1 more source
Frontiers in Genome Editing, 2021 Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind +4 more
doaj +1 more source
Cationic lipid-mediated intracellular delivery of antibodies into live cells
BioTechniques, 2008 The ability to introduce antibodies to live cells opens new insights to a wide range of applications, such as protein intracellular trafficking studies, protein interference studies with blocking antibodies, and live immunolabeling or protein ...
Claire O. Weill +2 more
doaj +1 more source
High efficiency transfection of thymic epithelial cell lines and primary thymic epithelial cells by Nucleofection [PDF]
, 2011 Thymic epithelial cells (TECs) are required for the development and differentiation of T cells and are sufficient for the positive and negative selection of developing T cells.
Brian G. Condie +2 more
core +2 more sources
Molecular Therapy: Nucleic Acids, 2013 RNA interference (RNAi) is a major tool for basic and applied investigations. However, obtaining RNAi data that have physiological significance requires investigation of regulations and therapeutic strategies in appropriate in vivo settings.
Sylvie Remaud +11 more
doaj +1 more source
Exploration of Targeted Anti-tumor Therapy, 2022 Aim: Gene-based immunotherapy against cancer is limited by low gene transfer efficiency. In the literature, interleukin-12 (IL-12) encoding plasmid associated with sonoporation has been shown to enhance antitumoral activity. Moreover, non-viral carriers
Hai Doan Do +10 more
doaj +1 more source
Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles. [PDF]
, 2018 PurposeThe delivery of transgenes into human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) represents an important tool in cardiac regeneration with potential for clinical applications.
Chavez, Karen S +11 more
core +2 more sources
Chinese Journal of Contemporary Neurology and Neurosurgery, 2021 Objective To investigate the anti⁃tumoral effect of ACT001, a novel antitumor compound, on the nuclear factor⁃κB (NF⁃κB) pathway and programmed cell death protein ligand 1 (PDL1) expression in U87 glioblastoma cell line.
ZHANG Jin⁃hao +9 more
doaj +1 more source
Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]
, 1996 We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D. +1 more
core +1 more source