Results 11 to 20 of about 595,494 (264)

Transfection of Platyhelminthes [PDF]

BioMed Research International, 2015
Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Moguel, Bárbara, Bobes, Raúl J., Carrero, Julio C., Laclette, Juan P.   +3 more
openaire   +2 more sources

Genome-Wide CRISPR-Cas9 Screen Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection

mSphere, 2020
Double-stranded RNA (dsRNA) is the hallmark of many viral infections. dsRNA is produced either by RNA viruses during replication or by DNA viruses upon convergent transcription.
Olivier Petitjean, Erika Girardi, Richard Patryk Ngondo, Vladimir Lupashin, Sébastien Pfeffer   +4 more
doaj   +1 more source

In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint

Biomedicines, 2021
Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics.
Lisa Sturm, Bettina Schwemberger, Ursula Menzel, Sonja Häckel, Christoph E. Albers, Christian Plank, Jaap Rip, Mauro Alini, Andreas Traweger, Sibylle Grad, Valentina Basoli   +10 more
doaj   +1 more source

Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins

Marine Drugs, 2023
The determination of the protein’s intracellular localization is essential for understanding its biological function. Protein localization studies are mainly performed on primary and secondary vertebrate cell lines for which most protocols have been ...
Kristina Dominko, Antea Talajić, Martina Radić, Nikolina Škrobot Vidaček, Kristian Vlahoviček, Maja Herak Bosnar, Helena Ćetković   +6 more
doaj   +1 more source

Generating Recombinant Antibodies to Membrane Proteins through Phage Display

Antibodies, 2016
One of the most important classes of proteins in terms of drug targets is cell surface membrane proteins, and yet it is a challenging set of proteins for generating high-quality affinity reagents.
Renhua Huang, Margaret M. Kiss, Melissa Batonick, Michael P. Weiner, Brian K. Kay   +4 more
doaj   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

Royal Society Open Science, 2022
Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics which has positively impacted ...
Camilo Pérez-Sosa, Anahí Sanluis-Verdes, Ariel Waisman, Antonella Lombardi, Gustavo Rosero, Alejandro La Greca, Shekhar Bhansali, Natalia Bourguignon, Carlos Luzzani, Maximiliano. S. Pérez, Santiago Miriuka, Betiana Lerner   +11 more
doaj   +1 more source

Green Fluorescent Protein is a Suitable Reporter of Tumor Hypoxia Despite an Oxygen Requirement for Chromophore Formation

Neoplasia: An International Journal for Oncology Research, 2001
The oxygen requirement for chromophore formation potentially limits the use of green fluorescent protein as a reporter under hypoxic conditions. In the light of this, the applicability of a hypoxia-responsive enhanced green fluorescent protein (EGFP ...
Dirk Vordermark, Toru Shibata, J. Martin Brown   +2 more
doaj   +1 more source

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Cells, 2020
In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte ...
Dinesh Bharti, Si-Jung Jang, Sang-Yun Lee, Sung-Lim Lee, Gyu-Jin Rho   +4 more
doaj   +1 more source

G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells

Pharmaceutics, 2022
Gene therapy is a promising approach for treating tumors. Conventional approaches of DNA delivery depending on non-viral or viral vectors are unsatisfactory due to the concerns of biosafety and cell-targeting efficiency.
Mengxi Xiang, Yongkui Li, Jia Liu, Jie Shi, Yizhi Ge, Chen Peng, Yawen Bin, Zheng Wang, Lin Wang   +8 more
doaj   +1 more source