Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs
Pharmaceutics, 2021 Nucleic acid and genetic medicines are increasingly being developed, owing to their potential to treat a variety of intractable diseases. A comprehensive understanding of the in vivo fate of these agents is vital for the rational design, discovery, and ...
Shintaro Fumoto +6 more
doaj +1 more source
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [PDF]
Methods and Protocols, 2020 Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer.
Sara E. Bodbin +2 more
openaire +3 more sources
Nature Communications, 2018 Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, and protect them from digestion by nuclease.
Shuang Peng +9 more
semanticscholar +1 more source
Biophysical and biological contributions of polyamine-coated carbon nanotubes and bidimensional buckypapers in the delivery of miRNAs to human cells [PDF]
, 2017 Recent findings in nanomedicine have revealed that carbon nanotubes (CNTs) can be used as potential drug carriers, therapeutic agents and diagnostics tools.
Bordi, Federico +9 more
core +2 more sources
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.
Journal of Biotechnology, 2015 CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. However, the delivery of Cas9 and synthesis of guide RNA (gRNA) remain as steps that can limit overall ...
Xiquan Liang +11 more
semanticscholar +1 more source
Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations.
Journal of the American Chemical Society, 2018 High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing "molecular string" RT (i.e., p-toluylsulfonyl ...
Huapan Fang +8 more
semanticscholar +1 more source
Plate-based transfection and culturing technique for genetic manipulation of
Malaria Journal, 2012 Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of ...
Caro Florence +2 more
doaj +1 more source
Functional differences between protamine preparations for the transfection of mRNA
Drug Delivery, 2020 Protamine is a natural cationic peptide mixture used as a drug for the neutralization of heparin and in formulations of slow-release insulin. In addition, Protamine can be used for the stabilization and delivery of nucleic acids (antisense, small ...
Natalia Teresa Jarzebska +7 more
doaj +1 more source
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
core +3 more sources
The magnetofection method: Using magnetic force to enhance gene delivery [PDF]
, 2003 In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles.
Alexiou C. +10 more
core +4 more sources