Results 31 to 40 of about 733,470 (308)

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.

Journal of Biotechnology, 2015
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. However, the delivery of Cas9 and synthesis of guide RNA (gRNA) remain as steps that can limit overall ...
Xiquan Liang, J. Potter, Shantanu Kumar, Y. Zou, R. Quintanilla, Mahalakshmi Sridharan, J. Carte, Wen Chen, Natasha Roark, S. Ranganathan, N. Ravinder, J. Chesnut   +11 more
semanticscholar   +1 more source

Hyperbranched Poly(β-amino ester)s (HPAEs) Structure Optimisation for Enhanced Gene Delivery: Non-Ideal Termination Elimination

Nanomaterials, 2022
Many polymeric gene delivery nano-vectors with hyperbranched structures have been demonstrated to be superior to their linear counterparts. The higher delivery efficacy is commonly attributed to the abundant terminal groups of branched polymers, which ...
Yinghao Li, Zhonglei He, Jing Lyu, Xianqing Wang, Bei Qiu, Irene Lara-Sáez, Jing Zhang, Ming Zeng, Qian Xu, Sigen A, James F. Curtin, Wenxin Wang   +11 more
doaj   +1 more source

Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations.

Journal of the American Chemical Society, 2018
High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing "molecular string" RT (i.e., p-toluylsulfonyl ...
Huapan Fang, Zhaopei Guo, Lin Lin, Jie Chen, Pingjie Sun, Jiayan Wu, Caina Xu, Huayu Tian, Xuesi Chen   +8 more
semanticscholar   +1 more source

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Magnetochemistry, 2023
In recent years, nanotechnology has deployed a new set of theragnostic tools, including magnetic resonance contrast agents, nano-delivery systems and magnetic hyperthermia treatments in cancer therapy, exploiting not only the small size of nanoparticles,
Manuel A. González-Gómez, Román Seco-Gudiña, Pelayo García-Acevedo, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, José Rivas   +6 more
doaj   +1 more source

Lipid droplet targeting domains of adipophilin

Journal of Lipid Research, 2003
Adipophilin (ADPH), a prominent protein component of lipid storage droplets (LSDs), is postulated to be necessary for the formation and cellular function of these structures.
James L. McManaman, William Zabaronick, Jerome Schaack, David J. Orlicky   +3 more
doaj   +1 more source

Size matters for in vitro gene delivery: investigating the relationships among complexation protocol, transfection medium, size and sedimentation

Scientific Reports, 2017
Although branched and linear polyethylenimines (bPEIs and lPEIs) are gold standard transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes and the composition of the transfection medium on their physicochemical ...
D. Pezzoli, Elisa Giupponi, D. Mantovani, G. Candiani   +3 more
semanticscholar   +1 more source

Efficient Delivery of SARS-CoV-2 Plasmid DNA in HEK-293T Cells Using Chitosan Nanoparticles

Pharmaceuticals
Background/Objectives: Gene therapy has emerged as a promising strategy for treating a wide range of diseases. However, a major challenge remains in developing efficient and safe delivery systems for genetic material. Nanoparticles, particularly chitosan
Citlali Cecilia Mendoza-Guevara, Alejandro Martinez-Escobar, María del Pilar Ramos-Godínez, José Esteban Muñoz-Medina, Eva Ramon-Gallegos   +4 more
doaj   +1 more source

Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene

Journal of Venomous Animals and Toxins including Tropical Diseases, 2007
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested.
P. Carnieli JR, A. M. Ventura, E. L. Durigon   +2 more
doaj   +1 more source

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Advancement of science, 2017
The objective of this study is to design a graphene‐based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex ...
C. Dou, N. Ding, F. Luo, T. Hou, Z. Cao, Yun Bai, Chuan Liu, Jianzhong Xu, S. Dong   +8 more
semanticscholar   +1 more source

Evaluation of Lipid-Based Transfection in Primary Monocytes Within an Ex Vivo Whole-Blood Model

Biomolecules
Transfection is a fundamental method in biomedical research to study intracellular molecular mechanisms by manipulating target protein expression. Various methods have been developed to deliver nucleic acids into the cells of interest in vitro, with ...
Robin Moolan-Vadackumchery, Lan Zhang, Frank Stüber   +2 more
doaj   +1 more source