Results 31 to 40 of about 686,707 (295)
Lipid droplet targeting domains of adipophilin
Journal of Lipid Research, 2003 Adipophilin (ADPH), a prominent protein component of lipid storage droplets (LSDs), is postulated to be necessary for the formation and cellular function of these structures.
James L. McManaman +3 more
doaj +1 more source
Efficient Delivery of SARS-CoV-2 Plasmid DNA in HEK-293T Cells Using Chitosan Nanoparticles
PharmaceuticalsBackground/Objectives: Gene therapy has emerged as a promising strategy for treating a wide range of diseases. However, a major challenge remains in developing efficient and safe delivery systems for genetic material. Nanoparticles, particularly chitosan
Citlali Cecilia Mendoza-Guevara +4 more
doaj +1 more source
Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Journal of Venomous Animals and Toxins including Tropical Diseases, 2007 A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested.
P. Carnieli JR +2 more
doaj +1 more source
Evaluation of Lipid-Based Transfection in Primary Monocytes Within an Ex Vivo Whole-Blood Model
BiomoleculesTransfection is a fundamental method in biomedical research to study intracellular molecular mechanisms by manipulating target protein expression. Various methods have been developed to deliver nucleic acids into the cells of interest in vitro, with ...
Robin Moolan-Vadackumchery +2 more
doaj +1 more source
Delivery of DNA-encoded vaccines and proteins in mice using cutaneous suction-mediated transfection. [PDF]
FEBS Open BioCutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Lallow EO +12 more
europepmc +2 more sources
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Magnetofection potentiates gene delivery to cultured endothelial cells [PDF]
, 2003 Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance
Sohn, Hae-Young +6 more
core +1 more source
Frontiers in Bioengineering and BiotechnologyBackgroundHuman bone marrow mesenchymal stem cell (MSC) administration reduces inflammation in pre-clinical models of sepsis and sepsis-related lung injury, however clinical efficacy in patients has not yet been demonstrated.
Rahul Y. Mahida +9 more
doaj +1 more source
Frontiers in Microbiology, 2020 Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from Sclerotinia sclerotiorum. This virus, named S.
Qihua Wang +10 more
doaj +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source