Results 51 to 60 of about 733,470 (308)

PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo

Frontiers in Pharmacology, 2023
mRNA-based vaccines and candidate therapeutics have great potential in various medical fields. For the delivery of mRNA into target cells and tissues, lipid formulations are often employed.
Kapilraj Periyasamy, Maria Maloverjan, Abhijit Biswas, Anu Remm, Martin Pook, Ana Rebane, Margus Pooga   +6 more
doaj   +1 more source

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice

Nature Communications, 2016
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery of CRISPR/Cas9 to the pancreas of adult mice, allowing ...
Roman Maresch, S. Mueller, C. Veltkamp, R. Öllinger, Mathias J Friedrich, I. Heid, K. Steiger, Julia Weber, T. Engleitner, Maxim Barenboim, Sabine Klein, Sandra Louzada, R. Banerjee, Alexander J. Strong, Teresa Stauber, Nina Gross, Ulf Geumann, S. Lange, M. Ringelhan, I. Varela, K. Unger, Fengtang Yang, R. Schmid, G. Vassiliou, R. Braren, G. Schneider, M. Heikenwalder, A. Bradley, D. Saur, R. Rad   +29 more
semanticscholar   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Plasmid DNA Delivery to Cancer Cells with Poly(L-lysine)-Based Copolymers Bearing Thermally Sensitive Segments: Balancing Polyplex Tightness, Transfection Efficiency, and Biocompatibility

Pharmaceutics
Background/Objectives. Efficient nucleic acid delivery into target cells remains a critical challenge in gene therapy. Due to its advantages in biocompatibility and safety, recent research has increasingly focused on non-viral gene delivery. Methods.
Mustafa Kotmakci, Natalia Toncheva-Moncheva, Sahar Tarkavannezhad, Bilge Debelec Butuner, Ivaylo Dimitrov, Stanislav Rangelov   +5 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal

Open Life Sciences, 2020
This study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders.
Li Chen, Qian Biao, Ni Zhao, Wang Qinzhang, Wang Zixiong, Ma Luping, Liu Zhili, Li Qiang, Wang Xinmin   +8 more
doaj   +1 more source

IL-21 Optimizes the CAR-T Cell Preparation Through Improving Lentivirus Mediated Transfection Efficiency of T Cells and Enhancing CAR-T Cell Cytotoxic Activities

Frontiers in Molecular Biosciences, 2021
Adoptive immunotherapy using CAR-T cells is a promising curative treatment strategy for hematological malignancies. Current manufacture of clinical-grade CAR-T cells based on lentiviral/retrovirus transfection of T cells followed by anti-CD3/CD28 ...
Li Du, Yaru Nai, Meiying Shen, Tingting Li, Jingjing Huang, Xiaojian Han, Wang Wang, Da Pang, Aishun Jin   +8 more
doaj   +1 more source

Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

Scientific Reports, 2016
Cell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value.
Y. Chow, Shuxun Chen, Ran Wang, Chichi Liu, C. Kong, Ronald A. Li, S. Cheng, Dong Sun   +7 more
semanticscholar   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Mineral coated microparticles doped with fluoride and complexed with mRNA prolong transfection in fracture healing

Frontiers in Bioengineering and Biotechnology
Introduction: Impaired fracture healing, specifically non-union, has been found to occur up to 14% in tibial shaft fractures. The current standard of care to treat non-union often requires additional surgeries which can result in long recovery times ...
Anna Laura Nelson, Anna Laura Nelson, Gianluca Fontana, Laura Chubb, Josh Choe, Katherine Williams, Katherine Williams, Dan Regan, Johnny Huard, Johnny Huard, William Murphy, William Murphy, Nicole Ehrhart, Chelsea Bahney, Chelsea Bahney, Chelsea Bahney   +15 more
doaj   +1 more source