Results 51 to 60 of about 971,477 (354)
Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2020 There were two objectives for this study. First, it focused on eight lipids having cholesterol as hydrophobic tails, carbamate linkers, and different polar headgroups.
Chopaka Thongbamrer +9 more
doaj +1 more source
Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral restoration of vision [PDF]
, 2012 Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for restoration of vision for treatment of retinitis pigmentosa (RP).
Edward Wong +5 more
core +2 more sources
Scientific Reports, 2016 Lipofectamine reagents are widely accepted as “gold-standard” for the safe delivery of exogenous DNA or RNA into cells. Despite this, a satisfactory mechanism-based explanation of their superior efficacy has remained mostly elusive thus far.
F. Cardarelli +9 more
semanticscholar +1 more source
Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents.
Bratislava Medical Journal, 2018 OBJECTIVES In this study, the optimal dose of Lipofectamine 3000 and Turbofect to transfect adherent cell lines such as CHO-K1 and HEK293 cells in comparison with non-adherent H9T-cells with pEGFP-N1 and pCDH was identified.
P. Rahimi +5 more
semanticscholar +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
BioengineeringElectroporation-mediated gene delivery is a cornerstone of synthetic biology, offering several advantages over other methods: higher efficiencies, broader applicability, and simpler sample preparation. Yet, electroporation protocols are often challenging
William R. Gaillard +12 more
doaj +1 more source
Intrabodies Binding the Proline-Rich Domains of Mutant Huntingtin Increase Its Turnover and Reduce Neurotoxicity [PDF]
, 2008 Although expanded polyglutamine (polyQ) repeats are inherently toxic, causing at least nine neurodegenerative diseases, the protein context determines which neurons are affected.
Bugg, Charles W. +5 more
core +2 more sources
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source
Cancer Management and Research, 2020 Yue-Lin Zhang,1,2,* Tan-Yang Zhou,1,2,* Jing Ai,3,* Sheng-Qun Chen,1,2,* Feng Chen,4 Chun-Hui Nie,1,2 Xin-Hua Chen,1,2 Guan-Hui Zhou,1,2 Hong-Liang Wang,1,2 Tong-Yin Zhu,1,2 Bao-Quan Wang,1,2 Zi-Niu Yu,1,2 Li Jing,1,2 Li-Ming Wu,1,2 Shu-Sen Zheng,1,2 Jun-
Zhang YL +15 more
doaj
Haunted Bodies: Cell Switching, Getting Lost and Adaptive Geographies [PDF]
Avant, 2017 This article proposes the ideas of stochastic resonance and noise as devices with which to think of the body or self as plural and porous. Boundaries and surfaces are proposed as indefinite; cell switching and narratives of the self are discussed in ...
Jane Grant, Joanne “Bob” Whalley
doaj +1 more source