Results 51 to 60 of about 692,115 (252)
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro
Frontiers in Bioengineering and Biotechnology, 2021 Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa +7 more
doaj +1 more source
KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer
Molecular Oncology, EarlyView.Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan +16 more
wiley +1 more source
Microdroplet Systems for Gene Transfer: From Fundamentals to Future Perspectives
MicromachinesMicrofluidics enables precise control of fluid movement within microchannels, facilitating the generation of microdroplets at high frequencies. This technology provides a unique platform for conducting biological and chemical experiments, enhancing ...
Mishell Criollo +4 more
doaj +1 more source
Liposomes as a gene delivery system
Brazilian Journal of Medical and Biological Research, 1999 Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle.
C. Ropert
doaj +1 more source
Sonoselective Transfection of Glioma Endothelium. [PDF]
Adv Sci (Weinh)Delivering non‐viral, gene‐bearing nanoparticles with thiol‐decorated surfaces to gliomas with focused ultrasound yields transfection tropism for endothelial cells. From this platform, “sonoselective” transfection of glioma endothelium is achieved via incorporation of an endothelial cell‐specific promoter.
Debski AC +8 more
europepmc +2 more sources
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker [PDF]
, 2009 Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X. +8 more
core
Partial transfection of cells using laminar flows in microchannels
, 2011 This manuscript describes a convenient method for partial transfection using a Y-shaped microchannel polydimethylsiloxane (PDMS)-glass chip and on-chip cationic lipid-mediated transfection.
Nie, Yong +19 more
core +1 more source
, 2022 High transfection efficiency and cell viability of immune cells with nanomaterials-based transfection reagent - Supplementary Table 1 Transfection efficiencies on day
Song Wang (144220), Dan Tian (697937)
core +1 more source
Pharmaceuticals, 2019 Systemic messenger RNA (mRNA) delivery, although still in its infancy, holds immense potential for application in cancer vaccination and immunotherapy. Its advantages over DNA transfection make it attractive in applications where transient expression is ...
Fiona Maiyo, Moganavelli Singh
doaj +1 more source