Results 41 to 50 of about 692,115 (252)

An Improved Approach to Protoplast Regeneration and Transfection in Banana (Musa acuminata AAA cv. Williams)

Applied Biosciences
Protoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo, Jean-Yves Paul, Anthony James, Rob Harding, James Dale   +4 more
doaj   +1 more source

PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo

Frontiers in Pharmacology, 2023
mRNA-based vaccines and candidate therapeutics have great potential in various medical fields. For the delivery of mRNA into target cells and tissues, lipid formulations are often employed.
Kapilraj Periyasamy, Maria Maloverjan, Abhijit Biswas, Anu Remm, Martin Pook, Ana Rebane, Margus Pooga   +6 more
doaj   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Plasmid DNA Delivery to Cancer Cells with Poly(L-lysine)-Based Copolymers Bearing Thermally Sensitive Segments: Balancing Polyplex Tightness, Transfection Efficiency, and Biocompatibility

Pharmaceutics
Background/Objectives. Efficient nucleic acid delivery into target cells remains a critical challenge in gene therapy. Due to its advantages in biocompatibility and safety, recent research has increasingly focused on non-viral gene delivery. Methods.
Mustafa Kotmakci, Natalia Toncheva-Moncheva, Sahar Tarkavannezhad, Bilge Debelec Butuner, Ivaylo Dimitrov, Stanislav Rangelov   +5 more
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Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal

Open Life Sciences, 2020
This study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders.
Li Chen, Qian Biao, Ni Zhao, Wang Qinzhang, Wang Zixiong, Ma Luping, Liu Zhili, Li Qiang, Wang Xinmin   +8 more
doaj   +1 more source

Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells

FEBS Letters, EarlyView.
DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park, Inho Kwon, Yong Hwan Kim, Ji‐Eun Bae, Joon Bum Kim, Seong Hyun Kim, Sungho Maeng, Doo Sin Jo, Dong‐Hyung Cho   +8 more
wiley   +1 more source

IL-21 Optimizes the CAR-T Cell Preparation Through Improving Lentivirus Mediated Transfection Efficiency of T Cells and Enhancing CAR-T Cell Cytotoxic Activities

Frontiers in Molecular Biosciences, 2021
Adoptive immunotherapy using CAR-T cells is a promising curative treatment strategy for hematological malignancies. Current manufacture of clinical-grade CAR-T cells based on lentiviral/retrovirus transfection of T cells followed by anti-CD3/CD28 ...
Li Du, Yaru Nai, Meiying Shen, Tingting Li, Jingjing Huang, Xiaojian Han, Wang Wang, Da Pang, Aishun Jin   +8 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Mineral coated microparticles doped with fluoride and complexed with mRNA prolong transfection in fracture healing

Frontiers in Bioengineering and Biotechnology
Introduction: Impaired fracture healing, specifically non-union, has been found to occur up to 14% in tibial shaft fractures. The current standard of care to treat non-union often requires additional surgeries which can result in long recovery times ...
Anna Laura Nelson, Anna Laura Nelson, Gianluca Fontana, Laura Chubb, Josh Choe, Katherine Williams, Katherine Williams, Dan Regan, Johnny Huard, Johnny Huard, William Murphy, William Murphy, Nicole Ehrhart, Chelsea Bahney, Chelsea Bahney, Chelsea Bahney   +15 more
doaj   +1 more source

Identification of Pfdhfr mutant variants in Plasmodium berghei model [PDF]

Maejo International Journal of Science and Technology, 2011
Parasite resistance to antimalarials is a major burden in controlling malaria disease. Genetic mutations within the parasites are found to be the factor in conferring resistance to drugs.
Chairat Uthaipibull
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