Results 41 to 50 of about 595,494 (264)

Mineral coated microparticles doped with fluoride and complexed with mRNA prolong transfection in fracture healing

Frontiers in Bioengineering and Biotechnology
Introduction: Impaired fracture healing, specifically non-union, has been found to occur up to 14% in tibial shaft fractures. The current standard of care to treat non-union often requires additional surgeries which can result in long recovery times ...
Anna Laura Nelson, Anna Laura Nelson, Gianluca Fontana, Laura Chubb, Josh Choe, Katherine Williams, Katherine Williams, Dan Regan, Johnny Huard, Johnny Huard, William Murphy, William Murphy, Nicole Ehrhart, Chelsea Bahney, Chelsea Bahney, Chelsea Bahney   +15 more
doaj   +1 more source

Identification of Pfdhfr mutant variants in Plasmodium berghei model [PDF]

Maejo International Journal of Science and Technology, 2011
Parasite resistance to antimalarials is a major burden in controlling malaria disease. Genetic mutations within the parasites are found to be the factor in conferring resistance to drugs.
Chairat Uthaipibull
doaj  

IL-21 Optimizes the CAR-T Cell Preparation Through Improving Lentivirus Mediated Transfection Efficiency of T Cells and Enhancing CAR-T Cell Cytotoxic Activities

Frontiers in Molecular Biosciences, 2021
Adoptive immunotherapy using CAR-T cells is a promising curative treatment strategy for hematological malignancies. Current manufacture of clinical-grade CAR-T cells based on lentiviral/retrovirus transfection of T cells followed by anti-CD3/CD28 ...
Li Du, Yaru Nai, Meiying Shen, Tingting Li, Jingjing Huang, Xiaojian Han, Wang Wang, Da Pang, Aishun Jin   +8 more
doaj   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Microdroplet Systems for Gene Transfer: From Fundamentals to Future Perspectives

Micromachines
Microfluidics enables precise control of fluid movement within microchannels, facilitating the generation of microdroplets at high frequencies. This technology provides a unique platform for conducting biological and chemical experiments, enhancing ...
Mishell Criollo, Gina Layedra, Camilo Pérez-Sosa, Gustavo Rosero, Ana Belén Peñaherrera-Pazmiño   +4 more
doaj   +1 more source

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

Frontiers in Bioengineering and Biotechnology, 2021
Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa, Pawel Prus, Pawel Prus, Karolina Bajdak-Rusinek, Paulina Teper, Katarzyna Gawron, Agnieszka Kowalczuk, Aleksander L. Sieron   +7 more
doaj   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Liposomes as a gene delivery system

Brazilian Journal of Medical and Biological Research, 1999
Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle.
C. Ropert
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Induction of translation-suppressive G3BP1+ stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA

hLife
Plasmid DNA transfection is one of the fundamental tools of biomedical research. Here, we found that plasmid DNA transfection mediated by liposomes activates multiple innate immune responses in several widely used cell lines.
Vladimir Majerciak, Zhi-Ming Zheng
doaj   +1 more source