Results 41 to 50 of about 971,477 (354)

A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems

Nucleic Acids Research, 2019
Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of ...
Jeremy J Gam, B. DiAndreth, Ross D. Jones, Jin Huh, Ron Weiss   +4 more
semanticscholar   +1 more source

Enhancing nucleic acid delivery by the integration of artificial intelligence into lipid nanoparticle formulation

Frontiers in Medical Technology
The advent of messenger RNA (mRNA) therapeutics has revolutionized medicine, with its potential underscored by rapid advancements during the COVID-19 pandemic.
Kagya Amoako, Amir Mokhammad, Afrida Malik, Sumith Yesudasan, Anas Wheba, Oluwanifemi Olagunju, Sean X. Gu, Sean X. Gu, Timur Yarovinsky, Edward Vincent S. Faustino, Juliane Nguyen, John Hwa   +11 more
doaj   +1 more source

Small poly-L-lysines improve cationic lipid-mediated gene transfer in vascular cells in vitro and in vivo [PDF]

, 2007
The potential of two small poly-L-lysines ( sPLLs), low molecular weight sPLL ( LMW-L) containing 7 - 30 lysine residues and L18 with 18 lysine repeats, to enhance the efficiency of liposome-mediated gene transfer ( GT) with cationic lipid DOCSPER {[}1,3-
Adam Golda, Armeanu S, Bauters C, Boussif O, Capan Y, Choukri Mekkaoui, Eastman SJ, Gao X, Groth D, Guenzburg WH, Hofling B, Huehns TY, Huehns TY, Jaroslav Pelisek, Khalil IA, Li S, Mahato RI, Markus G. Engelmann, Miller AD, Nikol S, Nikol S, Nikol S, Nikol S, Nikol S, Ogris M, Pelisek J, Pelisek J, Pelisek J, Pelisek J, Pierre-Henri Rolland, Rainer Klocke, Shi Y, Sigrid Nikol, Sorgi FL, Vitiello L, Wadhwa MS, Wolfert MA   +36 more
core   +1 more source

Size matters for in vitro gene delivery: investigating the relationships among complexation protocol, transfection medium, size and sedimentation

Scientific Reports, 2017
Although branched and linear polyethylenimines (bPEIs and lPEIs) are gold standard transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes and the composition of the transfection medium on their physicochemical ...
D. Pezzoli, Elisa Giupponi, D. Mantovani, G. Candiani   +3 more
semanticscholar   +1 more source

Chitosan-Based Polymeric Nanoparticles as an Efficient Gene Delivery System to Cross Blood Brain Barrier: In Vitro and In Vivo Evaluations

Pharmaceuticals
Significant progress has been made in the field of gene therapy, but effective treatments for brain tumors remain challenging due to their complex nature.
Ishaq N. Khan, Shiza Navaid, Walifa Waqar, Deema Hussein, Najeeb Ullah, Muhammad Umar Aslam Khan, Zakir Hussain, Aneela Javed   +7 more
doaj   +1 more source

Tetraethylenepentamine-Coated β Cyclodextrin Nanoparticles for Dual DNA and siRNA Delivery

Pharmaceutics, 2022
Nucleic acid reagents, including plasmid-encoded genes and small interfering RNA (siRNA), are promising tools for validating gene function and for the development of therapeutic agents.
Chi-Hsien Liu, Pei-Yin Shih, Cheng-Han Lin, Yi-Jun Chen, Wei-Chi Wu, Chun-Chao Wang   +5 more
doaj   +1 more source

Parkin interacts with Ambra1 to induce mitophagy [PDF]

, 2011
Mutations in the gene encoding Parkin are a major cause of recessive Parkinson's disease. Recent work has shown that Parkin translocates from the cytosol to depolarized mitochondria and induces their autophagic removal (mitophagy). However, the molecular
Cornelissen, Tom, De Strooper, Bart, Gevaert, Kris, Hofkens, Hilde, Mandemakers, Wim, Van Humbeeck, Cindy, Vandenberghe, Wim   +6 more
core   +2 more sources

Transfection of Platyhelminthes [PDF]

BioMed Research International, 2015
Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Moguel, Bárbara, Bobes, Raúl J., Carrero, Julio C., Laclette, Juan P.   +3 more
openaire   +2 more sources

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Advancement of science, 2017
The objective of this study is to design a graphene‐based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex ...
C. Dou, N. Ding, F. Luo, T. Hou, Z. Cao, Yun Bai, Chuan Liu, Jianzhong Xu, S. Dong   +8 more
semanticscholar   +1 more source

Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection

Applied Sciences, 2022
An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević, Verica Paunović, Maja Jovanović Tucović, Anja Tolić, Jovana Rajić, Svetlana Dinić, Aleksandra Uskoković, Nevena Grdović, Mirjana Mihailović, Ivanka Marković, Jelena Arambašić Jovanović, Melita Vidaković   +11 more
doaj   +1 more source