Results 41 to 50 of about 686,707 (295)

A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. [PDF]

, 2015
Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research.
Hart, S.L., Zhou, Haiyan, Munye, Mustafa M, Hart, Stephen L, Munye, MM, Munye MM, Tagalakis AD, Tagalakis, Aristides D, Tagalakis, Aristides, White, Edward A, White, EA, Castellaro, S, Zhou, H, Zhou H, Castellaro S, White EA, Bienemann, A, White, E.A., McCarthy, David, Munye, M.M., Castellaro, Sara, Tagalakis, AD, Hart, SL, Bienemann, Ali S, McCarthy, D, Bienemann A, Hart SL, McCarthy D   +27 more
core   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

An Improved Approach to Protoplast Regeneration and Transfection in Banana (Musa acuminata AAA cv. Williams)

Applied Biosciences
Protoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo, Jean-Yves Paul, Anthony James, Rob Harding, James Dale   +4 more
doaj   +1 more source

Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells

Frontiers in Bioengineering and Biotechnology, 2022
Nanodiscs are emerging to serve as transfer vectors for the insertion of recombinant membrane proteins into membranes of living cells. In combination with cell-free expression technologies, this novel process opens new perspectives to analyze the effects
Simon Umbach, Roman Levin, Sebastian Neumann, Torsten Steinmetzer, Volker Dötsch, Frank Bernhard   +5 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker [PDF]

, 2009
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X., Li, W., Li, J., Zhang, N., Liang, X., Zhang, X., Gong, P., Ma, G., Ryan, U.M.   +8 more
core  

Plasmid DNA Delivery to Cancer Cells with Poly(L-lysine)-Based Copolymers Bearing Thermally Sensitive Segments: Balancing Polyplex Tightness, Transfection Efficiency, and Biocompatibility

Pharmaceutics
Background/Objectives. Efficient nucleic acid delivery into target cells remains a critical challenge in gene therapy. Due to its advantages in biocompatibility and safety, recent research has increasingly focused on non-viral gene delivery. Methods.
Mustafa Kotmakci, Natalia Toncheva-Moncheva, Sahar Tarkavannezhad, Bilge Debelec Butuner, Ivaylo Dimitrov, Stanislav Rangelov   +5 more
doaj   +1 more source

PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo

Frontiers in Pharmacology, 2023
mRNA-based vaccines and candidate therapeutics have great potential in various medical fields. For the delivery of mRNA into target cells and tissues, lipid formulations are often employed.
Kapilraj Periyasamy, Maria Maloverjan, Abhijit Biswas, Anu Remm, Martin Pook, Ana Rebane, Margus Pooga   +6 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal

Open Life Sciences, 2020
This study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders.
Li Chen, Qian Biao, Ni Zhao, Wang Qinzhang, Wang Zixiong, Ma Luping, Liu Zhili, Li Qiang, Wang Xinmin   +8 more
doaj   +1 more source