Frontiers in Plant Science, 2022 In this study the anaphase promoting complex subunit CDC27a from Arabidopsis thaliana was introduced in the genome of Nicotiana benthamiana by Agrobacterium tumefaciens. The presence of the At-CDC27a gene facilitates plant biomass production. Compared to
Lilya Kopertekh, Sven Reichardt
doaj +1 more source
A conditional silencing suppression system for transient expression [PDF]
Scientific Reports, 2018 AbstractRNA silencing is a powerful tool deployed by plants against viral infection and abnormal gene expression. Plant viruses have evolved a suite of silencing suppressors for counter-defense, which are also widely used to boost transcript and protein accumulation in transient assays.
Rodrigo Siqueira Reis +4 more
openaire +5 more sources
Expression and function of transient receptor potential channels in the female bovine reproductive tract [PDF]
, 2016 © 2016 Elsevier Inc. The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions.
Atkin, Stephen L. +3 more
core +2 more sources
Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing. [PDF]
, 2016 RNA interference (RNAi) has emerged as a powerful way of reducing gene function in Drosophila melanogaster tissues. By expressing synthetic short hairpin RNAs (shRNAs) using the Gal4/UAS system, knockdown is efficiently achieved in specific tissues or in
Bosch, Justin A +2 more
core +1 more source
miRNA-based rapid differentiation of purified neurons from hPSCs advancestowards quick screening for neuronal disease phenotypes in vitro [PDF]
, 2020 Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs).
Aoyama, Takeshi +10 more
core +2 more sources
The human adenovirus 5 L4 promoter is activated by cellular stress response protein p53 [PDF]
, 2013 During adenovirus infection, the emphasis of gene expression switches from early genes to late genes in a highly regulated manner. Two gene products, L4-22K and L4-33K, contribute to this switch by activating the Major Late Transcription Unit (MLTU) and ...
Leppard, Keith, Wright, Jordan
core +1 more source
Beverage Plant Research, 2021 Specialized metabolites in tea (Camellia sinensis) are fundamental quality factors. It is important to characterize gene function in vivo to identify key enzymes and reactions involved in the biosynthesis of such metabolites.
Ying Zhou +3 more
doaj +1 more source
DsRed2 transient expression in Culex quinquefasciatus mosquitoes [PDF]
Memórias do Instituto Oswaldo Cruz, 2013 Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues.
Wilke, Andre Barretto Bruno +3 more
openaire +4 more sources
Expression of the Biologically Active Insulin Analog SCI-57 in Nicotiana Benthamiana
Frontiers in Pharmacology, 2019 Diabetes mellitus is a growing problem worldwide; however, only 23% of low-income countries have access to insulin, and ironically it costs higher in such countries than high-income ones.
Adriana Muñoz-Talavera +8 more
doaj +1 more source
Plant Molecular Farming as a Strategy Against COVID-19 – The Italian Perspective
Frontiers in Plant Science, 2020 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed more than 37,000 people in Italy and has caused widespread socioeconomic disruption.
Chiara Lico +10 more
doaj +1 more source