Results 61 to 70 of about 1,253,450 (306)

Optimization of Gene Gun-Mediated Transient Transformation and Explant Suitability in Coconut

Plants
Coconut genetic improvement remains challenging due to low regeneration efficiency and limited transformation success. We optimized major components of a gene gun-mediated transient transformation system and evaluated explant types to support future ...
Mingjun Ma, Hanlu Su, Hao Nie, Xiaomeng Fang, Saeed Rauf, Saira Batool, Yin Min Htwe, Dapeng Zhang, Peng Shi, Zhiying Li, Qun Yu, Xiangman He, Yong Wang   +12 more
doaj   +1 more source

Monoclonal Antibodies B38 and H4 Produced in Nicotiana benthamiana Neutralize SARS-CoV-2 in vitro

Frontiers in Plant Science, 2020
The ongoing coronavirus disease 2019 (COVID-19) outbreak caused by novel zoonotic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was initially reported in Wuhan city, Hubei Province of China, in late December 2019.
Balamurugan Shanmugaraj, Balamurugan Shanmugaraj, Kaewta Rattanapisit, Kaewta Rattanapisit, Suwimon Manopwisedjaroen, Arunee Thitithanyanont, Waranyoo Phoolcharoen, Waranyoo Phoolcharoen   +7 more
doaj   +1 more source

Transient-mediated fate determination in a transcriptional circuit of HIV [PDF]

, 2007
Steady-state behavior and bistability have been proposed as mechanisms for decision-making in gene circuits. However, transient gene expression has also been proposed to control cell fate with the decision arbitrated by the lifetime of the expression ...
Leor Weinberger, Michael Simpson, Roy D. Dar   +2 more
core   +1 more source

Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]

, 2004
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Christan, S., Laner, A., Schindelhauer, D., Schwarz, T.   +3 more
core   +1 more source

De Novo Synthesis of Anticholinergic Hyoscyamine and Scopolamine in Nicotiana benthamiana Based on Elucidating Tropane Alkaloid Biosynthetic Pathway of Anisodus luridus

Agronomy
Anisodus luridus, a perennial herb belonging to the genus Anisodus of the Solanaceae family, is an important Tibetan medicinal plant that produces pharmaceutical tropane alkaloids (TAs) including hyoscyamine and scopolamine. Its high yield of hyoscyamine
Mengling Wen, Junlan Zeng, Fei Qiu, Fangyuan Zhang, Zhihua Liao   +4 more
doaj   +1 more source

Evaluation of plant-produced Clostridium perfringens type D epsilon toxoid in a vaccine against enterotoxaemia in sheep

Onderstepoort Journal of Veterinary Research, 2017
Enterotoxaemia (pulpy kidney) is a common bacterial disease of sheep caused by Clostridium perfringens type D epsilon toxin. It has mortality rates of up to 30% in non-vaccinated animals.
Tinyiko Mokoena, Ereck Chakauya, Michael Crampton, Boet Weyers, Malefa Tselanyane, Tsepo Tsekoa, Rachel Chikwamba   +6 more
doaj   +1 more source

The Major Peanut Allergen Ara h 2 Produced in Nicotiana benthamiana Contains Hydroxyprolines and Is a Viable Alternative to the E. Coli Product in Allergy Diagnosis

Frontiers in Plant Science, 2021
Peanut allergy is a potentially life-threatening disease that is mediated by allergen-specific immunoglobulin E (IgE) antibodies. The major peanut allergen Ara h 2, a 2S albumin seed storage protein, is one of the most dangerous and potent plant ...
Öykü Üzülmez, Tanja Kalic, Tanja Kalic, Vanessa Mayr, Nina Lengger, Angelika Tscheppe, Christian Radauer, Christine Hafner, Christine Hafner, Wolfgang Hemmer, Heimo Breiteneder   +10 more
doaj   +1 more source

Integrins engage mitochondrial function for signal transduction by a mechanism dependent on Rho GTPases. [PDF]

, 2002
We show here the transient activation of the small GTPase Rac, followed by a rise in reactive oxygen species (ROS), as necessary early steps in a signal transduction cascade that lead to NFkappaB activation and collagenase-1 (CL-1)/matrix ...
Werb, Zena, Werner, Erica
core  

ER localized bestrophin1 activates Ca2+ dependent ion channels TMEM16A and SK4 [PDF]

, 2009
Bestrophins form Ca2+ activated Cl- channels and regulate intercellular Ca2+ signaling1. We demonstrate that bestrophin 1 is localized in the endoplasmic reticulum (ER), where it physically interacts with stromal interacting molecule 1 (Stim1), the ER ...
Fadi AlDehni, Jiraporn Ousingsawat, Joana Almaç, Karl Kunzelmann, Rainer Schreiber, Ralph Witzgall, René   +6 more
core   +2 more sources

LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development. [PDF]

, 2020
Developmental progression depends on temporally defined changes in gene expression mediated by transient exposure of lineage intermediates to signals in the progenitor niche.
Benner, Christopher W, Geusz, Ryan J, Harrington, Austin R, Jhala, Ulupi S, Matta, Ileana, Patel, Nisha A, Rosenfeld, Michael G, Sander, Maike, Shih, Hung-Ping, Vinckier, Nicholas K, Wang, Allen, Wang, Jianxun, Wang, Jinzhao, Wetton, Nichole, Wortham, Matthew   +14 more
core   +2 more sources