Results 81 to 90 of about 1,253,450 (306)

Venous Thromboembolism in Pediatric Bone Sarcoma Patients: A 10‐Year, Single‐Institution Experience Encompassing the COVID‐19 Pandemic

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Background Osteosarcoma (OS) and Ewing sarcoma (EWS) are the most common primary bone cancers in children, but acute thrombosis is poorly characterized in this population. Our study evaluated the rates of venous thromboembolism (VTE) and associated risk factors in pediatric patients with bone sarcomas treated over a 10‐year period encompassing
Sarah Kappa   +8 more
wiley   +1 more source

Rapid Investigation of Functional Roles of Genes in Regulation of Leaf Senescence Using Arabidopsis Protoplasts

open access: yesFrontiers in Plant Science, 2022
Leaf senescence is the final stage of leaf development preceding death, which involves a significant cellular metabolic transition from anabolism to catabolism.
Phan Phuong Thao Doan   +4 more
doaj   +1 more source

In Planta Transient Expression Systems for Monocots

open access: yes, 2015
The analysis of gene function in many monocotyledonous species has been held back to a certain extent by the difficulties of stable gene transformation. This chapter discusses the increasing use of transient in planta expression systems available for both RNA interference (RNAi)-mediated downregulation and overexpression of target genes of interest in ...
Lee, W-S.   +2 more
openaire   +1 more source

Effects of the Fluid Replacement Method During Online Hemodiafiltration on the Solute Removal Performance and Biocompatibility Using the Asymmetric Cellulose Triacetate Membrane

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Pre‐dilution online hemodiafiltration (Pre‐HDF) is predominantly used in Japan, whereas post‐dilution online HDF (Post‐HDF) is more common in Europe. An asymmetric cellulose triacetate (ATA) membrane may improve biocompatibility.
Kenji Sakurai   +4 more
wiley   +1 more source

An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

open access: yesPlants, 2019
In this study, we report the isolation and purification of protoplasts from Chinese kale (Brassica oleracea var. alboglabra) hypocotyls, and their transient gene expression transformation and subcellular localization of BaMYB75 (Bol042409). The upshot is
Bo Sun   +12 more
doaj   +1 more source

Temporal build-up of electromagnetically induced transparency and absorption resonances in degenerate two-level transitions [PDF]

open access: yes, 2002
The temporal evolution of electromagnetically induced transparency (EIT) and absorption (EIA) coherence resonances in pump-probe spectroscopy of degenerate two-level atomic transition is studied for light intensities below saturation.
A. Lezama   +26 more
core   +2 more sources

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

open access: yesFEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Transient Gene Expression by Nonintegrating Lentiviral Vectors

open access: yesMolecular Therapy, 2006
Nonintegrating lentiviral (NIL) vectors were produced from HIV-1-based lentiviral vectors by introducing combinations of mutations made to disable the integrase protein itself and to alter the integrase recognition sequences (att) in the viral LTR. NIL vectors with these novel combinations of mutations were used to transduce the human T lymphoid cell ...
Nightingale, Sarah J.   +7 more
openaire   +2 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

open access: yesFEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi   +5 more
wiley   +1 more source

Cloning of high molecular weight gluten subunit promoter and study on its function in wheat

open access: yesBrazilian Archives of Biology and Technology, 2009
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the ...
Jin Weibo   +3 more
doaj   +1 more source

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