Results 1 to 10 of about 5,895,543 (296)

An efficient Agrobacterium-mediated transient transformation system and its application in gene function elucidation in Paeonia lactiflora Pall

open access: yesFrontiers in Plant Science, 2022
Paeonia lactiflora Pall. is known as the king of herbaceous flowers with high ornamental and precious medicinal value. However, the lack of a stable genetic transformation system has greatly affected the research of gene function in P. lactiflora.
Shixin Guan   +10 more
doaj   +4 more sources

Step‐by‐step protocol for the isolation and transient transformation of hornwort protoplasts

open access: yesApplications in Plant Sciences, 2022
Premise A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte‐dominant life ...
Anna Neubauer   +8 more
doaj   +2 more sources

Optimization of Protoplast Preparation System from Leaves and Establishment of a Transient Transformation System in Apium graveolens

open access: yesAgronomy, 2023
Protoplast culture and transformation technology offer a novel method for developing new plant varieties. Nonetheless, the effective preparation of protoplasts and transformation technology specific to celery has yet to be achieved.
Jiageng Du   +7 more
doaj   +2 more sources

Protoplast Isolation and Transient Transformation System for Ginkgo biloba L.

open access: yesFrontiers in Plant Science, 2023
Ginkgo biloba L. has a unique evolutionary status. Owing to its high medicinal and ornamental value, ginkgo has also recently become a research hotspot.
Li'an Xu, Xin Han, Hao Rong
exaly   +2 more sources

In-Planta Transient Transformation of Avocado (Persea americana) by Vacuum Agroinfiltration of Aerial Plant Parts

open access: yesPlant Cell, Tissue and Organ Culture, 2023
Leaf age, wounding and jasmonic acid counter the recalcitrant nature of avocado, unlocking the use of Agrobacterium mediated transient transformation as a tool for functional genetics in this plant.
Stuart Casson, Rafael Urrea-López
exaly   +2 more sources

Establishment of Agrobacterium-Mediated Transient Transformation System in Sunflower

open access: yesPlants
Sunflower (Helianthus annuus L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding.
Fangyuan Chen   +9 more
doaj   +2 more sources

Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium

open access: yesFrontiers in Chemistry, 2018
Members of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists.
P. P. M. Heenatigala   +12 more
doaj   +3 more sources

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

open access: yesPlant Methods, 2018
Background Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well as the cultivated Gossypium ...
Haipeng Li   +7 more
doaj   +2 more sources

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

open access: yesBMC Research Notes, 2020
Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector ...
Rita Sharma   +5 more
doaj   +2 more sources

A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus. [PDF]

open access: yesPLoS ONE, 2015
Agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. However, the limitations of Agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants ...
Mitsuhiro Kimura   +2 more
doaj   +2 more sources

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