An efficient Agrobacterium-mediated transient transformation system and its application in gene function elucidation in Paeonia lactiflora Pall [PDF]
Frontiers in Plant Science, 2022 Paeonia lactiflora Pall. is known as the king of herbaceous flowers with high ornamental and precious medicinal value. However, the lack of a stable genetic transformation system has greatly affected the research of gene function in P. lactiflora.
Shixin Guan +10 more
doaj +4 more sources
Step‐by‐step protocol for the isolation and transient transformation of hornwort protoplasts [PDF]
Applications in Plant Sciences, 2022 Premise A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte‐dominant life ...
Anna Neubauer +8 more
doaj +2 more sources
Establishment of Agrobacterium-Mediated Transient Transformation System in Sunflower [PDF]
PlantsSunflower (Helianthus annuus L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding.
Fangyuan Chen +9 more
doaj +2 more sources
Establishment of Transient Transformation Systems in Welsh Onion (Allium fistulosum L.): Hairy Root Induction and Protoplast Transformation [PDF]
PlantsWelsh onion (Allium fistulosum L.), a globally significant vegetable, flavoring agent, and phytomedicine resource, has remained unavailable with established transient expression platforms for functional genomic investigations.
Dan Wang +9 more
doaj +2 more sources
CsCBDAS2-Driven Enhancement of Cannabinoid Biosynthetic Genes Using a High-Efficiency Transient Transformation System in Cannabis sativa ‘Cheungsam’ [PDF]
PlantsCannabis sativa produces pharmacologically valuable cannabinoids. In this study, we developed and optimized a transient transformation system using Cannabis sativa ‘Cheungsam’ to facilitate gene functional analysis.
Sang-Cheol Baek +6 more
doaj +2 more sources
Transformation of Transient Overvoltages by Inductive Voltage Transformers [PDF]
Sensors, 2021 Overvoltage transients occur after any type of switching activity in a power network, such as breaker operation, fault occurrence/clearance and rapid load change. This distortion of voltage is transformed to the secondary circuit of a voltage transformer.
Michał Kaczmarek, Dariusz Brodecki
doaj +3 more sources
Optimization of Gene Gun-Mediated Transient Transformation and Explant Suitability in Coconut [PDF]
PlantsCoconut genetic improvement remains challenging due to low regeneration efficiency and limited transformation success. We optimized major components of a gene gun-mediated transient transformation system and evaluated explant types to support future ...
Mingjun Ma +12 more
doaj +2 more sources
A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum) [PDF]
Plant Methods, 2018 Background Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well as the cultivated Gossypium ...
Haipeng Li +7 more
doaj +2 more sources
Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies [PDF]
BMC Research Notes, 2020 Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector ...
Rita Sharma +5 more
doaj +2 more sources
Frontiers in Chemistry, 2018 Members of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists.
P. P. M. Heenatigala +12 more
doaj +3 more sources