Step‐by‐step protocol for the isolation and transient transformation of hornwort protoplasts [PDF]
Applications in Plant Sciences, 2022 Premise A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte‐dominant life ...
Anna Neubauer +8 more
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Establishment of Agrobacterium-Mediated Transient Transformation System in Sunflower [PDF]
PlantsSunflower (Helianthus annuus L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding.
Fangyuan Chen +9 more
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CsCBDAS2-Driven Enhancement of Cannabinoid Biosynthetic Genes Using a High-Efficiency Transient Transformation System in Cannabis sativa ‘Cheungsam’ [PDF]
PlantsCannabis sativa produces pharmacologically valuable cannabinoids. In this study, we developed and optimized a transient transformation system using Cannabis sativa ‘Cheungsam’ to facilitate gene functional analysis.
Sang-Cheol Baek +6 more
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Establishment of Transient Transformation Systems in Welsh Onion (Allium fistulosum L.): Hairy Root Induction and Protoplast Transformation [PDF]
PlantsWelsh onion (Allium fistulosum L.), a globally significant vegetable, flavoring agent, and phytomedicine resource, has remained unavailable with established transient expression platforms for functional genomic investigations.
Dan Wang +9 more
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Transformation of Transient Overvoltages by Inductive Voltage Transformers [PDF]
Sensors, 2021 Overvoltage transients occur after any type of switching activity in a power network, such as breaker operation, fault occurrence/clearance and rapid load change. This distortion of voltage is transformed to the secondary circuit of a voltage transformer.
Michał Kaczmarek, Dariusz Brodecki
doaj +4 more sources
An efficient Agrobacterium-mediated transient transformation system and its application in gene function elucidation in Paeonia lactiflora Pall [PDF]
Frontiers in Plant Science, 2022 Paeonia lactiflora Pall. is known as the king of herbaceous flowers with high ornamental and precious medicinal value. However, the lack of a stable genetic transformation system has greatly affected the research of gene function in P. lactiflora.
Shixin Guan +10 more
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An Easy and Rapid Transformation Protocol for Transient Expression in Cotton Fiber
Frontiers in Plant Science, 2022 Cotton fiber is the most important natural textile material in the world. Identification and functional characterization of genes regulating fiber development are fundamental for improving fiber quality and yield. However, stable cotton transformation is
Xiaoguang Shang +6 more
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Optimization of Gene Gun-Mediated Transient Transformation and Explant Suitability in Coconut [PDF]
PlantsCoconut genetic improvement remains challenging due to low regeneration efficiency and limited transformation success. We optimized major components of a gene gun-mediated transient transformation system and evaluated explant types to support future ...
Mingjun Ma +12 more
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Frontiers in Chemistry, 2018 Members of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists.
P. P. M. Heenatigala +12 more
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A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum) [PDF]
Plant Methods, 2018 Background Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well as the cultivated Gossypium ...
Haipeng Li +7 more
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