Results 31 to 40 of about 548,529 (284)

A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis. [PDF]

open access: yesPLoS ONE, 2014
Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of ...
Kedong Xu   +13 more
doaj   +1 more source

Optimization of Protoplast Preparation System from Leaves and Establishment of a Transient Transformation System in Apium graveolens

open access: yesAgronomy, 2023
Protoplast culture and transformation technology offer a novel method for developing new plant varieties. Nonetheless, the effective preparation of protoplasts and transformation technology specific to celery has yet to be achieved.
Jiageng Du   +7 more
doaj   +1 more source

Protein transduction: A novel tool for tissue regeneration [PDF]

open access: yes, 2002
Tissue regeneration in humans is limited and excludes vitals organs like heart and brain. Transformation experiments with oncogenes like T antigen have shown that retrodifferentiation of the respective cells is possible but hard to control. To bypass the
Derer W.   +4 more
core   +1 more source

Transient Transformation of Plants

open access: yes, 2009
Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins.
Jones, H. D., Doherty, A., Sparks, C. A.
openaire   +2 more sources

Transient and steady-state shear banding in a lamellar phase as studied by Rheo-NMR [PDF]

open access: yes, 2012
Flow fields and shear-induced structures in the lamellar (L-alpha) phase of the system triethylene glycol mono n-decyl ether (C10E3)/water were investigated by NMR velocimetry, diffusometry, and H-2 NMR spectroscopy. The transformation from multilamellar
Galvosas, Petrik   +4 more
core   +1 more source

No influence of dabigatran anticoagulation on hemorrhagic transformation in an experimental model of ischemic stroke [PDF]

open access: yes, 2012
Background: Dabigatran etexilate (DE) is a new oral direct thrombin inhibitor. Clinical trials point towards a favourable risk-to-benefit profile of DE compared to warfarin. In this study, we evaluated whether hemorrhagic transformation (HT) occurs after
Bohmann, Ferdinand   +6 more
core   +3 more sources

EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings

open access: yesFrontiers in Plant Science, 2019
The Catharanthus roseus plant is the exclusive source of the valuable anticancer terpenoid indole alkaloids, vinblastine (VB) and vincristine (VC). The recent availability of transcriptome and genome resources for C.
Samuel Mortensen   +8 more
doaj   +1 more source

AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings [PDF]

open access: yes, 2014
Background: Transient gene expression via Agrobacterium-mediated DNA transfer offers a simple and fast method to analyze transgene functions. Although Arabidopsis is the most-studied model plant with powerful genetic and genomic resources, achieving ...
Chen, Chao-Ying   +8 more
core   +2 more sources

Isolation of Haustorium Protoplasts Optimized by Orthogonal Design for Transient Gene Expression in Phelipanche aegyptiaca

open access: yesPlants
The efficient protoplast transient transformation system in plants is an important tool to study gene expression, metabolic pathways, and various mutagenic parameters, but it has not been established in Phelipanche aegyptiaca.
Xiaojian Zeng   +8 more
doaj   +1 more source

Single-cell-based system to monitor carrier driven cellular auxin homeostasis [PDF]

open access: yes, 2013
Background: Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular
Barbez, Elke   +7 more
core   +3 more sources

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