Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops [PDF]
Frontiers in Plant Science, 2021 Versatile protoplast platforms greatly facilitate the development of modern botany. However, efficient protoplast-based systems are still challenging for numerous horticultural plants and crops.
Rui Ren +10 more
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Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu) [PDF]
BMC Plant Biology, 2023 Background Areca palm (Areca catechu) is a woody perennial plant of both economical and medicinal importance grown in tropical and subtropical climates.
Yaodi Wang +9 more
doaj +2 more sources
Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation [PDF]
Journal of Genetic Engineering and Biotechnology, 2021 Background Protoplasts (i.e., naked plant cells) can be used for in vitro manipulations and genetic improvement in cultivars with economic value. During the last decade, protoplast research in economic brown algae has been scarce, and it is usually ...
Jose Avila-Peltroche +2 more
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Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Glycine max [L.] Merr.) [PDF]
Frontiers in Plant ScienceSoybean is important crop species in agriculture, food science, and biotechnology due to their valuable components. The exploration of soybean genetic traits is being highlighted for the advancement of research in various aspects.
Kihwan Kim +7 more
doaj +2 more sources
Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars [PDF]
Plants, 2023 Protoplasts are a versatile tool in plant biotechnology since they can be used for basic biological studies as well as for breeding strategies based on genome editing.
Ester Stajič
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A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L [PDF]
BMC Plant BiologyBackground Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk +3 more
doaj +2 more sources
Enhancing Protoplast Isolation and Early Cell Division from Cannabis sativa Callus Cultures via Phenylpropanoid Inhibition [PDF]
PlantsDe novo regeneration of Cannabis sativa L. (cannabis) using tissue culture techniques remains unreliable and infrequent. Conventional methods for the regeneration and transformation of cannabis have not achieved the reliability and replicability that ...
Adrian S. Monthony +1 more
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Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose [PDF]
Biology, 2020 Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in ...
Hyun Hee Kang +2 more
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Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.) [PDF]
Plant Methods, 2018 Background An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes along with the study of proteins ...
S. V. G. N. Priyadarshani +12 more
doaj +2 more sources
An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1 [PDF]
Plant Methods, 2017 Background Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene ...
Guohui Yu +5 more
doaj +2 more sources