Protoplast Regeneration and Its Use in New Plant Breeding Technologies
Frontiers in Genome Editing, 2021 The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need to feed a growing global population.
Bastiaan Bargmann
exaly +4 more sources
Optimization of protoplast regeneration in the model plant Arabidopsis thaliana
Plant Methods, 2021 Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration.
Yeong Yeop Jeong +4 more
doaj +2 more sources
Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture, 2021 Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed +10 more
doaj +2 more sources
Biology, 2020 Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in ...
Aung Htay Naing
exaly +2 more sources
Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea
Mycobiology, 2019 Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc.
Jyh-Ching Chou
exaly +2 more sources
Protoplast cultures and protoplast fusion focused on Brassicaceae: A review
Horticultural Science, 2004 The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-
B. Navrátilová
doaj +2 more sources
Plant Science, 2017 The protoplast retracts during apoptosis-like programmed cell death (AL-PCD) and, if this retraction is an active component of AL-PCD, it should be used as a defining feature for this type of programmed cell death. We used an array of pharmacological and
Cara Daly +2 more
exaly +2 more sources
Establishment of the Genetic Transformation System of Trichoderma longibrachiatum 40418 and Its Induced Resistance Against Meloidogyne incognita in Tomato [PDF]
Journal of FungiTrichoderma longibrachiatum is a filamentous fungus that functions as a biocontrol agent against multiple plant diseases. However, the lack of a genetic transformation system has hindered studies on gene function and the underlying biocontrol mechanisms ...
Ning Luo +5 more
doaj +2 more sources
A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Fragaria × ananassa) [PDF]
PlantsClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a simple and powerful tool, which enables gene knockout or insertion of new gene cassettes. This method has been applied to various plants and
Yunsun Kim +4 more
doaj +2 more sources
An Efficient and Stable PEG-Mediated Transformation System for Medicinal Fungus Ophiocordyceps xuefengensis: Optimization and Functional Validation [PDF]
Journal of FungiOphiocordyceps xuefengensis is an important medicinal fungus with considerable pharmaceutical and economic value. However, its industrial and scientific utilization has been severely limited by the lack of an efficient genetic transformation system ...
Xiaoting Feng +6 more
doaj +2 more sources