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Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (Populus alba × Populus glandulosa) [PDF]
BiologyIn poplar, the protracted stable genetic transformation procedure constrains rapid gene functional analyses. To address this limitation, we optimized a protocol for the high-yield isolation and efficient transient transformation of protoplasts from ...
Chao Yu +3 more
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Establishment of the Genetic Transformation System of Trichoderma longibrachiatum 40418 and Its Induced Resistance Against Meloidogyne incognita in Tomato [PDF]
Journal of FungiTrichoderma longibrachiatum is a filamentous fungus that functions as a biocontrol agent against multiple plant diseases. However, the lack of a genetic transformation system has hindered studies on gene function and the underlying biocontrol mechanisms ...
Ning Luo +5 more
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Frontiers in Genome Editing, 2021 Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind +4 more
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BMC Plant Biology, 2023 Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek +4 more
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Optimization of protoplast regeneration in the model plant Arabidopsis thaliana
Plant Methods, 2021 Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration.
Yeong Yeop Jeong +4 more
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Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture, 2021 Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed +10 more
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Journal of Genetic Engineering and Biotechnology, 2021 Background Protoplasts (i.e., naked plant cells) can be used for in vitro manipulations and genetic improvement in cultivars with economic value. During the last decade, protoplast research in economic brown algae has been scarce, and it is usually ...
Jose Avila-Peltroche +2 more
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BMC Research Notes, 2023 Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela +4 more
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Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application
Frontiers in Genome Editing, 2021 In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA ...
Jin-Jun Yue +6 more
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Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012 For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant withdesired traits. For this purpose, protoplast preparation is a first and important step.
Esmaeil CHAMANI +5 more
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