Results 1 to 10 of about 54,280 (357)

A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L [PDF]

BMC Plant Biology
Background Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk, Kamil Szymonik, Andrew Maxwell Phineas Jones, Ewa Grzebelus   +3 more
doaj   +2 more sources

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review [PDF]

Horticultural Science, 2004
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-
B. Navrátilová
doaj   +2 more sources

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection [PDF]

Frontiers in Genome Editing
Protoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes.
Ryan Creeth, Andrew Thompson, Zoltan Kevei   +2 more
doaj   +2 more sources

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

Frontiers in Genome Editing, 2021
Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind, Xueyuan Li, Emelie Ivarson, Annelie Ahlman, Li-Hua Zhu   +4 more
doaj   +1 more source

Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn

BMC Plant Biology, 2023
Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek, Reneé Pérez-Pérez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus   +4 more
doaj   +1 more source

Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Plant Methods, 2021
Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration.
Yeong Yeop Jeong, Hun-Young Lee, Suk Weon Kim, Yoo-Sun Noh, Pil Joon Seo   +4 more
doaj   +1 more source

Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.

Agriculture, 2021
Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed, Miao Miao, Emmanouil D. Pratsinakis, Hongliang Zhang, Wei Wang, Yuan Yuan, Meiling Lyu, Junaid Iftikhar, Ahmed F. Yousef, Panagiotis Madesis, Binghua Wu   +10 more
doaj   +1 more source

PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry. [PDF]

Plant Methods
Majumder S, Igamberdiev AU, Debnath SC.
europepmc   +3 more sources

Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation

Journal of Genetic Engineering and Biotechnology, 2021
Background Protoplasts (i.e., naked plant cells) can be used for in vitro manipulations and genetic improvement in cultivars with economic value. During the last decade, protoplast research in economic brown algae has been scarce, and it is usually ...
Jose Avila-Peltroche, Boo Yeon Won, Tae Oh Cho   +2 more
doaj   +1 more source

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +8 more sources