Results 11 to 20 of about 26,488 (218)

Protoplast as a tool to address questions in plant physiology [PDF]

Journal of Plant Development, 2018
A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way ...
Tim XING, Lining TIAN
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Plant Breeding Through Protoplast Fusion [PDF]

Jurnal Biologi Universitas Andalas, 2020
Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time.
Devi Armita
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Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage

BMC Plant Biology
Background Methylation is a major DNA modification contributing to the epigenetic regulation of nuclear gene expression and genome stability. DNA methyltransferases (DNMT) inhibitors are widely used in epigenetic and cancer research, but their biological
Agnieszka Kiełkowska, Agnieszka Brąszewska   +1 more
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An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.
Xiaoxiao Yu, Jintai Yu, Yin Lu, Wenjing Li, Guanzhong Huo, Jun Zhang, Yan Li, Jianjun Zhao, Jun Li   +8 more
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DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection [PDF]

Frontiers in Genome Editing
Protoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes.
Ryan Creeth, Andrew Thompson, Zoltan Kevei   +2 more
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Research Progress of Algae Protoplast Fusion Technique [PDF]

, 2022
This paper discussed the technique of algal protoplast fusion, including the method of protoplast-induced fusion, the screening and identification of hybrid cells and so on.
XU, Xiaoying, LI, Lei, CAO, Yanan, LU, Xiaoguang, LIU, Peng, SHI, Wenkai   +5 more
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Protoplast Isolation and Transformation in Oil

, 2022
187202The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol (PEG)-mediated transfection and DNA microinjection, highlighting also the critical steps associated with the ...
Noll, Gundula, Ghulam Kadir Ahmad Parveez, Dirk Pruefer, Mat Yunus Abdul Masani, Gundula Noll, M. D. Piji Mohd Al Akmarul Fizree, Parveez, Ghulam Kadir Ahmad, Ravigadevi Sambanthamurthi, Fizree, M. D. Piji Mohd Al Akmarul, Sambanthamurthi, Ravigadevi, Masani, Mat Yunus Abdul, Prüfer, Dirk   +11 more
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Development of an efficient leaf protoplast isolation and transient expression system for Artemisia japonica

Biologia Plantarum, 2023
Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng, X. Fu, B. Peng, Q. Miao, S. Zeng, K. Tang, Q. Pan   +6 more
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Protoplast suspension culture.

, 2022
A. Freshly isolated protoplast. B. Dividing protoplast three days after culture initiation. C. Microcalli developing two weeks after culture initiation. D. Minicalli 4 weeks after culture initiation. E. Minicalli developing on solid medium.
Asunta Mukami (14011413), Mathew Ngugi (10494139), Richard Oduor (7408637), Bicko Steve Juma (14011410), Wilton Mwema Mbinda (14204647), Cecilia Mweu (10494133)   +5 more
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Solutions used for protoplast isolation and transfection.

, 2023
Solutions used for protoplast isolation and transfection.
Michael J. Thomson (8477751), Endang M. Septiningsih (6003947), Aya Bridgeland (15188045), Sudip Biswas (8506077), Nikolaos Tsakirpaloglou (15188048)   +4 more
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