Results 11 to 20 of about 54,280 (357)
BMC Research Notes, 2023 Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela +4 more
doaj +1 more source
One Protoplast Is Not the Other! [PDF]
Plant Physiology, 2011 Protoplasts are widely used to study a variety of cellular processes because they are easy to transform, manipulate and image at high resolution. Protoplasts are thought to loose their original cell identity and therefore their origin is of minor unimportance.
FARACO M +4 more
openaire +4 more sources
Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application
Frontiers in Genome Editing, 2021 In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA ...
Jin-Jun Yue +6 more
doaj +1 more source
Biologia Plantarum, 2023 Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng +6 more
doaj +1 more source
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012 For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant withdesired traits. For this purpose, protoplast preparation is a first and important step.
Esmaeil CHAMANI +5 more
doaj +1 more source
Asymmetric somatic plant hybridization : status and applications [PDF]
, 2013 To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product.
Deryckere, Dieter +4 more
core +2 more sources
Protoplast as a tool to address questions in plant physiology [PDF]
Journal of Plant Development, 2018 A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way ...
Tim XING, Lining TIAN
doaj +1 more source
Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting [PDF]
, 2012 After initiation of the leaf primordium, biomass accumulation is controlled mainly by cell proliferation and expansion in the leaves1. However, the Arabidopsis leaf is a complex organ made up of many different cell types and several structures.
Buchanan-Wollaston, Vicky +4 more
core +1 more source
Makara Journal of Science, 2014 The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production.
Wijanarka Wijanarka +3 more
doaj +1 more source
Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells [PDF]
, 2008 Tomato spotted wilt virus (TSWV) particles are spherical and enveloped, an uncommon feature among plant infecting viruses. Previous studies have shown that virus particle formation involves the enwrapment of ribonucleoproteins with viral glycoprotein ...
Denecke, J. +5 more
core +2 more sources