Results 11 to 20 of about 56,071 (291)

Protoplast as a tool to address questions in plant physiology [PDF]

Journal of Plant Development, 2018
A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way ...
Tim XING, Lining TIAN
doaj   +3 more sources

Plant Breeding Through Protoplast Fusion [PDF]

Jurnal Biologi Universitas Andalas, 2020
Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time.
Devi Armita
doaj   +2 more sources

Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage

BMC Plant Biology
Background Methylation is a major DNA modification contributing to the epigenetic regulation of nuclear gene expression and genome stability. DNA methyltransferases (DNMT) inhibitors are widely used in epigenetic and cancer research, but their biological
Agnieszka Kiełkowska, Agnieszka Brąszewska   +1 more
doaj   +2 more sources

Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre)

Frontiers in Genome Editing, 2021
Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind, Xueyuan Li, Emelie Ivarson, Annelie Ahlman, Li-Hua Zhu   +4 more
doaj   +1 more source

Stochastic gene expression drives mesophyll protoplast regeneration

Science Advances, 2021
Isolating protoplasts allows stochastic gene expression to enable regeneration of differentiated cells at a low frequency. Cell pluripotency is fundamental to biology.
Mengxue Xu, Qingwei Du, Caihuan Tian, Ying Wang, Yuling Jiao   +4 more
semanticscholar   +1 more source

DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration

Horticulture Research, 2022
CRISPR/Cas9 genome editing technology can overcome many limitations of traditional breeding, offering enormous potential for crop improvement and food production.
Samaneh Najafi, E. Bertini, E. D'Incà, M. Fasoli, S. Zenoni   +4 more
semanticscholar   +1 more source

Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops

Frontiers in Plant Science, 2021
Versatile protoplast platforms greatly facilitate the development of modern botany. However, efficient protoplast-based systems are still challenging for numerous horticultural plants and crops.
R. Ren, Jie Gao, D. Yin, Kai Li, Chuqiao Lu, Sagheer Ahmad, Yonglu Wei, Jianpeng Jin, Gen-Fa Zhu, Fengxi Yang   +9 more
semanticscholar   +1 more source

Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn

BMC Plant Biology, 2023
Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek, Reneé Pérez-Pérez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus   +4 more
doaj   +1 more source

Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

Frontiers in Plant Science, 2021
Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.).
Xue-Yuan Li, Sjur Sandgrind, Oliver J. Moss, Rui Guan, E. Ivarson, Eu-Sheng Wang, Selvaraju Kanagarajan, Li-Hua Zhu   +7 more
semanticscholar   +1 more source

Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation

Journal of Genetic Engineering and Biotechnology, 2021
Background Protoplasts (i.e., naked plant cells) can be used for in vitro manipulations and genetic improvement in cultivars with economic value. During the last decade, protoplast research in economic brown algae has been scarce, and it is usually ...
Jose Avila-Peltroche, Boo Yeon Won, Tae Oh Cho   +2 more
doaj   +1 more source