Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.) [PDF]
Frontiers in Plant Science, 2021 Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.).
Sjur Sandgrind +2 more
exaly +8 more sources
Optimization of protoplast regeneration in the model plant Arabidopsis thaliana [PDF]
Plant Methods, 2021 Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration.
Yeong Yeop Jeong +4 more
doaj +3 more sources
Establishment of an Efficient Protoplast Regeneration and Transfection Protocol for Field Cress (Lepidium campestre) [PDF]
Frontiers in Genome Editing, 2021 Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind +4 more
doaj +6 more sources
Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata [PDF]
Frontiers in Plant ScienceBrassica carinata is an important oil crop with significant potential for food and industrial production. The application of the CRISPR/Cas9 genome editing tool in B. carinata could accelerate its breeding cycle.
Xueyuan Li +4 more
doaj +7 more sources
Protoplast Regeneration and Its Use in New Plant Breeding Technologies [PDF]
Frontiers in Genome Editing, 2021 The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need to feed a growing global population.
Kelsey M. Reed, Bastiaan O. R. Bargmann
doaj +2 more sources
Optimization of protoplast cell division and microcallus formation toward somatic embryogenesis in oil palm [PDF]
Frontiers in Plant ScienceBackgroundEfficient protoplast regeneration remains a major obstacle to the application of somatic hybridization, genome editing and precision breeding in oil palm (Elaeis guineensis); while early developmental stages, from cell wall regeneration and ...
Mohamed A. A. Ahmed +8 more
doaj +2 more sources
Red and Far-Red LED Lighting Enhances Protoplast-to-Plant Regeneration in Broccoli (Brassica oleracea var. italica) [PDF]
PlantsPlants have a remarkable ability to regenerate tissues and organs from single cells, a property that underpins in vitro protoplast regeneration. Efficient protoplast-to-plant regeneration remains a major bottleneck for genome engineering in many crop ...
Miriam Romero-Muñoz +4 more
doaj +2 more sources
A novel application of Raman spectroscopy to monitor cell wall removal during protoplasting in grapevine [PDF]
Frontiers in Plant ScienceIntroductionGrapevine is one of the most widely cultivated and economically important perennial fruit crops worldwide. However, its genetic improvement is severely constrained by a long juvenile phase and a high degree of recalcitrance in in vitro ...
Edoardo Bertini +9 more
doaj +2 more sources
A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L [PDF]
BMC Plant BiologyBackground Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk +3 more
doaj +2 more sources
Efficient isolation and transient transformation of cowpea protoplasts [PDF]
BMC Plant BiologyBackground Cowpea (Vigna unguiculata) is an important legume crop, but reproducible protoplast isolation and transient expression systems remain limited.
Xuewen Qiu +7 more
doaj +2 more sources