Results 21 to 30 of about 393,999 (153)

Enhancing Protoplast Isolation and Early Cell Division from Cannabis sativa Callus Cultures via Phenylpropanoid Inhibition

Plants
De novo regeneration of Cannabis sativa L. (cannabis) using tissue culture techniques remains unreliable and infrequent. Conventional methods for the regeneration and transformation of cannabis have not achieved the reliability and replicability that ...
Adrian S. Monthony, Andrew Maxwell P. Jones   +1 more
doaj   +2 more sources

Exogenous Nitric Oxide Delays Plant Regeneration from Protoplast and Protonema Development in Physcomitrella patens

Plants, 2020
Nitric oxide (NO) has been recognized as a major player in the regulation of plant physiology and development. NO regulates cell cycle progression and cell elongation in flowering plants and green algae, although the information about NO function in non ...
Daniela Cervantes-Pérez, Angélica Ortega-García, Rigoberto Medina-Andrés, Ramón Alberto Batista-García, Verónica Lira-Ruan   +4 more
doaj   +2 more sources

Phytosulfokine Peptide Library: Chemical Synthesis and Biological Evaluation on Protoplast Regeneration [PDF]

, 2023
Phytosulfokine (PSK) is a highly conserved plant peptide hormone (PPH) containing two sulfated tyrosine residues. PSK acts as a growth factor in Arabidopsis thaliana plant species and significantly improves the efficiency of cellular proliferation in ...
Dieuwertje, Streefkerk, Richard, Immink, Jasper, van de Sande, Bauke, Albada, Martijn, Fiers   +4 more
core   +2 more sources

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Frontiers in Genome Editing, 2021
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA ...
Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin   +6 more
doaj   +1 more source

Plant regeneration from leaf mesophyll derived protoplasts of cassava (Manihot esculenta Crantz)

PLoS ONE, 2022
A high yield of isolated protoplast and reliable regeneration system are prerequisite for successful somatic hybridization and genome editing research.
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Ngugi, Richard Oduor, Wilton Mwema Mbinda   +5 more
doaj   +2 more sources

A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts

Plants, 2021
Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization.
Ki-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim   +8 more
doaj   +1 more source

Intermolecular Carbon–Carbon Naphthalene Coupling Involves a Radical Mechanism Driven by Cytochrome P450 in Alternaria alternata

Angewandte Chemie, EarlyView.
1,8‐DHN is an important intermediate to form complex aromatic ring systems. Phenol‐coupling reactions are important for the dimerization of two molecules. Here, we show that a fungal cytochrome P450 enzyme catalyzes specific C─C bonds. ABSTRACT Dihydroxynaphthalene (DHN) derivatives are precursors of biologically important molecules, including melanin ...
Jia Gao, Leyao Chen, Karin Steffen, Henrik Schweder, Birgit Schreckenberger, Shu‐Ming Li, Ben Auxier, Antonis Rokas, Reinhard Fischer   +8 more
wiley   +2 more sources

Protonema induction, transient transformation, and protoplast regeneration in the peat moss Sphagnum papillosum

, 2023
Sphagnum mosses are important carbon sequesters and emerging model organisms. However, induction and long-term cultivation of thalloid protonema in several species was not achievable so far.
Kaltenbrunner, Alena, Decker, Eva L., Lüth, Volker, Pascal, Arnaud, Reski, Ralf   +4 more
core   +1 more source

Establishment of a Genetic Transformation System in Guanophilic Fungus Amphichorda guana

Journal of Fungi, 2021
Fungi from unique environments exhibit special physiological characters and plenty of bioactive natural products. However, the recalcitrant genetics or poor transformation efficiencies prevent scientists from systematically studying molecular biological ...
Min Liang, Wei Li, Landa Qi, Guocan Chen, Lei Cai, Wen-Bing Yin   +5 more
doaj   +1 more source

Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn

BMC Plant Biology, 2023
Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek, Reneé Pérez-Pérez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus   +4 more
doaj   +1 more source