Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture, 2021 Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed +10 more
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Plant Breeding Through Protoplast Fusion [PDF]
Jurnal Biologi Universitas Andalas, 2020 Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time.
Devi Armita
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ACTIN7 Is Required for Perinuclear Clustering of Chloroplasts during Arabidopsis Protoplast Culture [PDF]
Plants, 2020 In Arabidopsis, the actin gene family comprises eight expressed and two non-expressed ACTIN (ACT) genes. Of the eight expressed isoforms, ACT2, ACT7, and ACT8 are differentially expressed in vegetative tissues and may perform specific roles in ...
Michael B. Sheahan +3 more
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Optimization of protoplast cell division and microcallus formation toward somatic embryogenesis in oil palm [PDF]
Frontiers in Plant ScienceBackgroundEfficient protoplast regeneration remains a major obstacle to the application of somatic hybridization, genome editing and precision breeding in oil palm (Elaeis guineensis); while early developmental stages, from cell wall regeneration and ...
Mohamed A. A. Ahmed +8 more
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BMC Research Notes, 2023 Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela +4 more
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A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L [PDF]
BMC Plant BiologyBackground Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk +3 more
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A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Fragaria × ananassa) [PDF]
PlantsClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a simple and powerful tool, which enables gene knockout or insertion of new gene cassettes. This method has been applied to various plants and
Yunsun Kim +4 more
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Impedance flow cytometry for rapid quality assessment of protoplast cultures [PDF]
Plant MethodsBackground Protoplasts, which are plant cells devoid of cell walls, are valuable tools in plant biotechnology. However, they are highly sensitive to mechanical and osmotic stress during isolation and early culture, often leading to significant loss of ...
Tiago Rodrigues +7 more
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Frontiers in Genome Editing, 2021 Field cress (Lepidium campestre) is a potential oilseed crop that has been under domestication in recent decades. CRISPR/Cas9 is a powerful tool for rapid trait improvement and gene characterization and for generating transgene-free mutants using ...
Sjur Sandgrind +4 more
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Optimization of protoplast regeneration in the model plant Arabidopsis thaliana
Plant Methods, 2021 Background Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration.
Yeong Yeop Jeong +4 more
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