Results 11 to 20 of about 3,956,989 (242)

Plant regeneration via protoplast electrofusion in cassava

Journal of Integrative Agriculture, 2020
Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava.
Feng WEN, Wen-pan SU, Hua ZHENG, Ben-chi YU, Zeng-feng MA, Peng ZHANG, Wen-wu GUO   +6 more
doaj   +2 more sources

Enhancing Protoplast Isolation and Early Cell Division from Cannabis sativa Callus Cultures via Phenylpropanoid Inhibition

Plants
De novo regeneration of Cannabis sativa L. (cannabis) using tissue culture techniques remains unreliable and infrequent. Conventional methods for the regeneration and transformation of cannabis have not achieved the reliability and replicability that ...
Adrian S. Monthony, Andrew Maxwell P. Jones   +1 more
doaj   +2 more sources

Comparison of the regeneration ability in leaf-derived protoplast cultures of the cultivated Apiaceae

Scientia Horticulturae
Protoplast-based technology is widely applied across many economically important plants. However, in the Apiaceae family, studies on protoplast-to-plant regeneration systems have primarily focused on carrot and a few other crop species.
Ewa Grzebelus, Aneta Malec-Pala, Katarzyna Stelmach-Wityk, Dariusz Kadluczka, Emilia Morańska, Tomasz Oleszkiewicz   +5 more
doaj   +2 more sources

Establishment of Pluripotent Cell Cultures to Explore Allelopathic Activity of Coffee Cells by Protoplast Co-Culture Bioassay Method

Plants, 2020
We focused on the demonstration of a new pluripotent coffee cell culture system to control the growth and metabolic functions. Somatic cells in the epidermal layer of in vitro somatic embryos (SEs) of Coffea canephora expressed higher pluripotency to ...
Shinjiro Ogita, Muchamad Imam Asrori, Hamako Sasamoto   +2 more
doaj   +2 more sources

Enhancement of Antagonism through Protoplast Fusion in Trichoderma spp

Journal of Pure and Applied Microbiology, 2017
The filamentous fungi Trichoderma is used asmost important biocontrol agent. Because of the absence of sexual reproduction in this fungus, other methods of genetic improvement have been developed to enhance its bicontrol potential.In the present ...
B.P. Chandrappa, M.P. Basavarajappa
doaj   +2 more sources

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Biology, 2020
Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in ...
Hyun Hee Kang, Aung Htay Naing, Chang Kil Kim   +2 more
doaj   +2 more sources

Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage

BMC Plant Biology
Background Methylation is a major DNA modification contributing to the epigenetic regulation of nuclear gene expression and genome stability. DNA methyltransferases (DNMT) inhibitors are widely used in epigenetic and cancer research, but their biological
Agnieszka Kiełkowska, Agnieszka Brąszewska   +1 more
doaj   +2 more sources

Embryogenic Stem Cell Identity after Protoplast Isolation from Daucus carota and Recovery of Regeneration Ability through Protoplast Culture. [PDF]

Int J Mol Sci, 2022
Protoplasts are single cells isolated from tissues or organs and are considered a suitable system for cell studies in plants. Embryogenic cells are totipotent stem cells, but their regeneration ability decreases or becomes lost altogether with extension ...
Han JE, Lee HS, Lee H, Cho H, Park SY.
europepmc   +2 more sources

Soybean Protoplast Culture and Direct Gene Uptake and Expression by Cultured Soybean Protoplasts [PDF]

Plant Physiology, 1987
A method was developed for culturing protoplasts freshly isolated from developing soybean (Glycine max L.) cotyledons. First cell divisions were observed within 5 days after protoplast isolation and microcalli, consisting of about 20 cells, were formed within 10 days.
W, Lin, J T, Odell, R M, Schreiner
openaire   +2 more sources

Protoplast suspension culture.

, 2022
A. Freshly isolated protoplast. B. Dividing protoplast three days after culture initiation. C. Microcalli developing two weeks after culture initiation. D. Minicalli 4 weeks after culture initiation. E. Minicalli developing on solid medium.
Asunta Mukami (14011413), Mathew Ngugi (10494139), Richard Oduor (7408637), Bicko Steve Juma (14011410), Wilton Mwema Mbinda (14204647), Cecilia Mweu (10494133)   +5 more
core   +1 more source