Results 41 to 50 of about 3,956,989 (242)

Dual Repression by IPA1 Fine‐Tunes OsbZIP79‐Mediated Salt Tolerance in Rice

Advanced Science, EarlyView.
Dual repression by IPA1 fine‐tunes OsbZIP79‐mediated salt tolerance in rice: direct transcriptional inhibition under normal conditions and salt‐induced degradation under stress. This dual mechanism activates OsbZIP79 to regulate Na+/K+ homeostasis and redox balance via downstream genes, enabling optimal salt stress response.
Hui Wang, Bo Liu, Can Hu, Chenfan Zheng, Yuting Wei, Xier Xu, Wei Yang, Yu Fang, Guangle Shen, Pedro García‐Caparros, Conghe Zhang, Qingyao Shu, Meng Jiang   +12 more
wiley   +1 more source

Factors affecting mango (Mangifera indica L.) protoplast isolation and culture

, 2011
The major factors influencing protoplast isolation and culture of mango (Mangifera indica L.) cv. Kensington Pride were investigated. The resultant protocol was used to compare plating efficiency among 4 mango cultivars.
Richard R. Williams, Ramezan Rezazadeh, Rezazadeh, Ramezan, Dion K. Harrison, Harrison, Dion K., Williams, Richard R.   +5 more
core   +1 more source

A Scalable Droplet Microfluidic Platform That Overcomes Large‐Cell Constraints for Long‐Term Plant Cell Culture and Embryogenesis Analysis

Advanced Science, EarlyView.
This work presents a large‐cell‐compatible droplet microfluidic platform that overcomes long‐standing barriers in plant cell culture by enabling high‐fidelity encapsulation, novel multilayer 3D droplet incubation for scalable long‐term culture, and support for the full developmental progression of large plant cells.
Arman Mirmiran, Fangchi Shao, Gregory Datto, Tathansh Joshi, Elizabeth Chatt, Yashar Bashirzadeh, Dan Stessman, Lai Wei, Kuangwen Hsieh, Hena Guo, Tza‐Huei Wang   +10 more
wiley   +1 more source

Cotyledon-derived diploid and haploid protoplast culture and diploid plant regeneration in Brassica napus cv. ' Topas '

, 1998
The present paper describes a simple and reliable protocol for the successful isolation, purification, culture, and regeneration of diploid cotyledon-derived protoplasts of Brassica napus L. cv. 'Topas'.
H Kieft, M Sun, AAM van Lammeren
core   +1 more source

Blue Light Receptor PHR2‐Mediated Spectrum Antiviral Immunity

Advanced Science, EarlyView.
Blue light suppresses RBSDV through the blue‐light receptor ZmPHR2, which interacts with ZmISP in chloroplasts to trigger ROS burst and antiviral defense. The viral effector P6 enhances ZmPHR2–ZmISP interaction, alters their distribution, and suppresses ROS.
Runyi Chen, Yuxin Tai, Xinyu Wang, Chunhua Mu, Xia Liu, Gongjian Li, Jiayi Liu, Xin Lu, Duo Shi, Zhiqiang Zhou, Jienan Han, Zhuanfang Hao, Mingshun Li, Degui Zhang, Hongjun Yong, Zhennan Xu, Jianfeng Weng, Xinhai Li   +17 more
wiley   +1 more source

Formation of Bacterionema matruchotii Protoplast

, 1979
Protoplast formation with Bacterionema matruchotii was studied turbidimetrically and microscopically. The protoplasts were inducible during culture by addition of glycine, lysozyme and Ficoll to a medium containing Tween-80 and polyethylene glycol.
Toshiko Itoyama, Yasunaga Kadomura, Ichiro Takazoe   +2 more
core   +1 more source

Chrysanthemum Morifolium–Derived Nanovesicles Reduce Lactate‐Associated Histone Lactylation and Modulate Microglia‐Mediated Neuroinflammation in Sepsis‐Associated Encephalopathy

Advanced Science, EarlyView.
When used to treat sepsis‐associated encephalopathy, Chrysanthemum morifolium–derived nanovesicles can inhibit the H3K18la/NF‐κB axis to regulate microglia‐mediated neuroinflammation. ABSTRACT Sepsis‐associated encephalopathy (SAE)—a complication of the central nervous system in patients with sepsis—markedly diminishes cognitive functions and is linked
Kaiqiang Li, Xuling Luo, Xiaoting Zhang, Chengwen He, Wei Xu, Aibo Xu, Shushan Hua, Liping Wang, Liang Ma, Ke Hao, Youmin Ying, Zhen Wang   +11 more
wiley   +1 more source

Protoplast culture and plant regeneration of different agronomically important Brassica species and varieties

Agricultural and Food Science, 1991
Protoplast cultures were prepared from 6-day-old hypocotyls of six spring, seven winter cultivars of Brassica napus L. and one line of Brassica campestris L.
János Pauk, Sándor Fekete, Juha Vilkki, Seppo Pulli   +3 more
doaj  

A design-of-experiments framework for protoplast culture media optimization

Scientific Reports
Plant protoplast culture is a critical enabling technology for genome editing, functional genomics, and plant regeneration, yet culture medium optimization remains largely empirical due to the large number of interacting variables. Here, we implemented a
Tiago Rodrigues, Robin Lardon, Mária Šimášková, Steffen Vanneste, Danny Geelen   +4 more
doaj   +1 more source

Phytosulfokine alpha enhances regeneration of transformed and untransformed protoplasts of Brassica oleracea

Frontiers in Plant Science
Phytosulfokine-α (PSK-α) is a disulfated pentapeptide (YIYTQ) acting as an intercellular signal peptide and growth factor. It was originally isolated from conditioned medium of asparagus mesophyll cell cultures in 1996 and later characterized as a ...
Valentin Vogrinčič, Damijana Kastelec, Jana Murovec   +2 more
doaj   +1 more source