Results 61 to 70 of about 3,956,989 (242)

Deciphering argumentation on the regulation of genome‐edited crops in academic publications: A systematic review

PLANTS, PEOPLE, PLANET, EarlyView.
Genome editing technologies such as CRISPR‐Cas are reshaping debates about the future of agriculture, yet disagreement over how these crops should be regulated remains widespread. This study clarifies the range of arguments used in scientific discussions about genome‐edited crops, making underlying assumptions and value judgements more visible.
Philippe Mathys, Marco De Pascali Pepe, Bernice Elger, Bettina M. Zimmermann   +3 more
wiley   +1 more source

Determinação de metodologia para oisolamento de protoplastos de tangerina Cleópatra (Citrus reshni Hort.) Methodology choice for protoplast isolation in Cleopatra mandarin (Citrus reshni Hort.)

Scientia Agricola, 1995
A hibridação somática via fusão de protoplastos vem sendo utilizada no melhoramento de porta-enxertos de citros em diversos países. Nos Estados Unidos, vários estudos demonstram a eficiência de procedimentos no isolamento e cultivo de protoplastos dessa ...
R.P. de Oliveira, B.M.J. Mendes, A. Tulmann Neto   +2 more
doaj   +1 more source

Liquid Phase Backscattered Scanning Electron Microscopy of Bacillus subtilis Spores

Small Methods, EarlyView.
Graphene encapsulation enables liquid‐phase BSE‐SEM to visualize subsurface architecture of whole, hydrated Bacillus subtilis spores without staining. This approach is applied to visualize dormant spores and subsequent structural changes during spore germination, while imaging across a range of beam energies and Monte Carlo simulations provide a ...
Jack Bromley, Adrián Pedrazo‐Tardajos, Yongqiang Meng, Matthew C. Spink, Dogan Ozkaya, Rodney S. Ruoff, Graham Christie, Angus I. Kirkland, Judy S. Kim   +8 more
wiley   +1 more source

Jatropha curcas protoplast isolation and inhibitory effects of culture [PDF]

, 2016
Improvement on the sub-tropical plant Jatropha curcas may increase the use of this drought tolerant oilseed crop for the production of biodiesel. One of the methods for enhancing a species is through genetic manipulation of the protoplast.
Lukaszewski, Christopher S.
core   +1 more source

Allelopathy in a Leguminous Mangrove Plant, : Protoplast Co-culture Bioassay and Rotenone Effect

Natural Product Communications, 2015
To investigate allelopathic activity of a leguminous mangrove plant, Derris indica , the ‘Protoplasts Co-culture Method’ for bioassay of allelopathy was developed using suspension culture.
Aya Inoue, Daisuke Mori, Reiko Minagawa, Yoshiharu Fujii, Hamako Sasamoto   +4 more
doaj   +1 more source

Approaches to Superefficient Yeast Transformations: DNA Phase Separation, Cell Growth State and Systematic Experimental Design

Yeast, EarlyView.
Yeast transformations are mechanistically poorly understood and could be made orders of magnitude more efficient by three approaches. (1) Investigate mechanisms of DNA uptake including DNA phase separation. (2) Account for growth state. (3) Use systematic statistical design of experiments to test combinations of factors that enhance transformation ...
Alistair Elfick, Edward W. J. Wallace
wiley   +1 more source

The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum

BMC Plant Biology
Background Due to the totipotency of plant cells, which allows them to reprogram from a differentiated to a dedifferentiated state, plants exhibit a remarkable regenerative capacity, including under in vitro culture conditions.
Magdalena Zaranek, Artur Pinski, Bozena Skupien-Rabian, Urszula Jankowska, Kamila Godel-Jedrychowska, Katarzyna Sala-Cholewa, Katarzyna Nowak, Ewa Kurczyńska, Ewa Grzebelus, Alexander Betekhtin   +9 more
doaj   +1 more source

Peroxisomes contribute to reactive oxygen species homeostasis and cell division induction in Arabidopsis protoplasts

Frontiers in Plant Science, 2015
The ability to induce Arabidopsis protoplasts to de-differentiate and divide provides a convenient system to analyse organelle dynamics in plant cells acquiring totipotency.
Terence Wei Yang Tiew, Michael B. Sheahan, Ray J. Rose   +2 more
doaj   +1 more source

A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis

Yeast, EarlyView.
ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley   +1 more source

Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L). II. Natural fluorescence and direct somatic embryogenesis from protoplasts

Acta Societatis Botanicorum Poloniae, 2014
The development of protoplast derived from somatic embryos and some of their characteristics were compared with embryos from suspension and in vivo in the same B line.
W. Burza, B. Woźniak, J. A. Tarkowska, S. Malepszy   +3 more
doaj   +1 more source