Results 81 to 90 of about 3,956,989 (242)

Plant cis‐regulatory grammar: Decoding the multidimensional code of transcriptional regulation for programmable crop engineering

Journal of Integrative Plant Biology, EarlyView.
This review decodes the grammar of plant cis‐regulatory elements, revealing that their function emerges from sequence, chromatin accessibility, 3D topology, and cell type context. It highlights how single‐cell omics, CRISPR dissection, and AI‐driven design converge to enable predictive engineering and crop improvement. ABSTRACT Cis‐regulatory elements (
Libin Zhang, Maoteng Li
wiley   +1 more source

Morphogenic cell and protoplast cultures of tritordeum

, 1993
Immature embryo- and anther-derived suspension and protoplast cultures were established in hexaploid tritordeum. The morphogenic capability of these cultures was studied under different nitrogen regimes. Both embryo- and anther-derived suspensions showed
Martín, Antonio, Martin, A., Barcelo, P., Lorz, H., Rallo, Luis, Hernández Molina, Pilar, Hernandez, P., Lazzeri, P. A.   +7 more
core   +1 more source

Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of Brassica carinata

Frontiers in Plant Science
Brassica carinata is an important oil crop with significant potential for food and industrial production. The application of the CRISPR/Cas9 genome editing tool in B. carinata could accelerate its breeding cycle.
Xueyuan Li, Misteru Tesfaye, Sjur Sandgrind, Rui Guan, Li-Hua Zhu   +4 more
doaj   +1 more source

An OsSK12/13‐OsERF60‐OsHsfA2c module regulates heat stress tolerance in rice

Journal of Integrative Plant Biology, EarlyView.
The heat‐induced GSK3‐like kinases OsSK12 and OsSK13 phosphorylate and stabilize the transcription factor OsERF60, which activates the expression of downstream genes including the heat shock factor gene OsHsfA2c. This regulatory module governs heat‐responsive gene induction, representing a mechanistic target for enhancing crop resilience under climate ...
Juan Gao, Zi‐Wei Yao, Zhi‐Ping Deng, Jing‐Jing Wang, Dong‐Jie Zhu, Dai‐Xi Yue, Hai‐Ping Lu, Jian‐Xiang Liu   +7 more
wiley   +1 more source

Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium

, 2007
Vital protoplasts from Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were isolated from both somatic embryos and leaves. The highest yields were obtained when 1.5% cellulase, 0.5% macerase and 0.5% driselase were used for Spathiphyllum
Werbrouck, Stefaan, Van Huylenbroeck, Johan, Duquenne, Barbara, Eeckhaut, Tom   +3 more
core   +1 more source

PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp

Journal of Biological Researches, 2014
This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material
Edu Setiti Wida Utami
doaj  

TaANT11‐4A enhances grain yield by orchestrating photosynthesis and carbohydrate metabolism through direct transcriptional activation

Journal of Integrative Plant Biology, EarlyView.
TaANT11‐4A is a positive regulator of wheat grain size and yield, directly activating TaEGY2, TaAAE1, and TaAAE2 to enhance photosynthesis and carbohydrate metabolism. The favorable Hap2 haplotype increases TaANT11‐4A expression and grain size, providing a genetic resource for breeding higher‐yielding wheat varieties.
Pengpeng Zhang, Jingjing Wen, Shan Gao, Jiajun Ma, Jiahui Jin, Xiaofei Ma, Wen‐Ting Liu, Juan Li, De‐Jun Han, Yue‐Qiang Zhang, Kun‐Ming Chen   +10 more
wiley   +1 more source

Disruption of OsMAPKKK24 activation by the Xanthomonas oryzae effector XopY leads to compromised disease resistance in rice

Journal of Integrative Plant Biology, EarlyView.
The kinase OsRLCK55 phosphorylates OsMAPKKK24 to positively regulate rice resistance against Xanthomonas oryzae. To compromise this defense, the effector XopY targets OsMAPKKK24 and suppresses its autophosphorylation and the transphosphorylation mediated by OsRLCK55, ultimately leading to reduced disease resistance.
Lilan Chen, Yongming Chen, Wencai Xu, Xiaofang Wu, Shanshan Deng, Zhanchun Wang, Dingzhong Tang, Wei Wang   +7 more
wiley   +1 more source

Plant regeneration from protoplasts of Gentiana straminea Maxim

Open Life Sciences, 2016
A protocol is described for plant regeneration from protoplasts of Gentiana straminea Maxim. via somatic embryogenesis. Protoplasts were isolated from embryogenic calli in an enzyme solution composed of 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10, 0 ...
Shi Guomin, Yang Lina, He Tao
doaj   +1 more source

Visualising Physcomitrium patens protoplasts with full‐rotation soft X‐ray tomography

Journal of Microscopy, EarlyView.
Abstract Soft X‐ray tomography (SXT) is a label‐free, high‐resolution imaging modality that is optimal for 3D visualisation of whole hydrated cells. Although SXT has been extensively used to study remodelling in more than 130 mammalian cell lines, its application to plants remains largely unexplored. In this study, we present the first approach at full‐
Fariha Mahzabin Annesha, Sadia S. Tamanna, Katharina Grasser, Stefanie J. Müller‐Schüssele, Venera Weinhardt   +4 more
wiley   +1 more source