Results 1 to 10 of about 58,172 (308)

Optimization of Protoplast Preparation and Establishment of Genetic Transformation System of an Arctic-Derived Fungus Eutypella sp.

Frontiers in Microbiology, 2022
Arctic-derived fungus Eutypella sp. D-1 has attracted wide attention due to its huge ability to synthesize secondary metabolites. However, current studies only focus on stimulating its production of new secondary metabolites by OSMAC strategies, and the ...
Yaodong Ning, Bo Hu, Haobing Yu, Xiaoyu Liu, Binghua Jiao, Xiaoling Lu   +5 more
doaj   +1 more source

Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts

Microbial Cell, 2020
We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane ...
Rintaro Tsuchikado, Satoshi Kami, Sawako Takahashi, Hiromi Nishida   +3 more
doaj   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source

Ligand-induced monoubiquitination of BIK1 regulates plant immunity [PDF]

, 2020
The detection of microorganism-associated ligands by plant cells activates a signalling cascade in which the kinase BIK1 is monoubiquinated, released from the FLS2-BAK1 complex, and internalized by endocytosis. Recognition of microbe-associated molecular
Alves Neubus Claus, Lucas, He, Ping, Heese, Antje, Leslie, Michelle E., Li, Bo, Liu, Jun, Ma, Xiyu, Peng, Junmin, Russinova, Eugenia, Savatin, Daniel, Shan, Libo, Tao, Kai, Tyler, Brett M., Wu, Zhiping, Yu, Xiao, Zhou, Jinggeng   +15 more
core   +1 more source

One Protoplast Is Not the Other!   [PDF]

Plant Physiology, 2011
Protoplasts are widely used to study a variety of cellular processes because they are easy to transform, manipulate and image at high resolution. Protoplasts are thought to loose their original cell identity and therefore their origin is of minor unimportance.
FARACO M, DI SANSEBASTIANO, Gian Pietro, SPELT K, KOES RE, QUATTROCCHIO F.M.   +4 more
openaire   +4 more sources

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +8 more sources

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry

Biosensors, 2022
A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells.
Xingda Dai, Shuaihua Zhang, Siyuan Liu, Hang Qi, Xuexin Duan, Ziyu Han, Jiehua Wang   +6 more
doaj   +1 more source

Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting [PDF]

, 2012
After initiation of the leaf primordium, biomass accumulation is controlled mainly by cell proliferation and expansion in the leaves1. However, the Arabidopsis leaf is a complex organ made up of many different cell types and several structures.
Buchanan-Wollaston, Vicky, Eyres, Alison, Gifford, Miriam L., Grønlund, Jesper T., Kumar, Sanjeev   +4 more
core   +1 more source

Asymmetric somatic plant hybridization : status and applications [PDF]

, 2013
To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product.
Deryckere, Dieter, Eeckhaut, Tom, Lakshmanan, Prabhu Shankar, Van Bockstaele, Erik, Van Huylenbroeck, Johan   +4 more
core   +2 more sources

Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells [PDF]

, 2008
Tomato spotted wilt virus (TSWV) particles are spherical and enveloped, an uncommon feature among plant infecting viruses. Previous studies have shown that virus particle formation involves the enwrapment of ribonucleoproteins with viral glycoprotein ...
Denecke, J., Foresti, O., Goldbach, R.W., Kormelink, R.J.M., Ribeiro, D.M.O.G., Wellink, J.   +5 more
core   +2 more sources