Results 1 to 10 of about 38,339 (281)

Optimization of Protoplast Preparation and Establishment of Genetic Transformation System of an Arctic-Derived Fungus Eutypella sp.

Frontiers in Microbiology, 2022
Arctic-derived fungus Eutypella sp. D-1 has attracted wide attention due to its huge ability to synthesize secondary metabolites. However, current studies only focus on stimulating its production of new secondary metabolites by OSMAC strategies, and the ...
Yaodong Ning, Bo Hu, Haobing Yu, Xiaoyu Liu, Binghua Jiao, Xiaoling Lu   +5 more
doaj   +1 more source

Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts

Microbial Cell, 2020
We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane ...
Rintaro Tsuchikado, Satoshi Kami, Sawako Takahashi, Hiromi Nishida   +3 more
doaj   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source

One Protoplast Is Not the Other!   [PDF]

Plant Physiology, 2011
Protoplasts are widely used to study a variety of cellular processes because they are easy to transform, manipulate and image at high resolution. Protoplasts are thought to loose their original cell identity and therefore their origin is of minor unimportance.
FARACO M, DI SANSEBASTIANO, Gian Pietro, SPELT K, KOES RE, QUATTROCCHIO F.M.   +4 more
openaire   +4 more sources

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry

Biosensors, 2022
A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells.
Xingda Dai, Shuaihua Zhang, Siyuan Liu, Hang Qi, Xuexin Duan, Ziyu Han, Jiehua Wang   +6 more
doaj   +1 more source

Optimized methods for random and targeted mutagenesis in field pea (Pisum sativum L.)

Frontiers in Plant Science, 2022
Field pea is an important pulse crop for its dense nutritional profile and contribution to sustainable agricultural practices. Recently, it has received extensive attention as a potential leading source of plant-based proteins.
Prashant Kumar Pandey, Pankaj Bhowmik, Sateesh Kagale   +2 more
doaj   +1 more source

Mitochondrial Genome of Callus Protoplast Has a Role in Mesophyll Protoplast Regeneration in Citrus: Evidence From Transgenic GFP Somatic Homo-Fusion

Horticultural Plant Journal, 2017
Protoplast fusion has great potential in citrus improvement. Although citrus mesophyll protoplasts usually cannot divide and regenerate, symmetric protoplast fusion of embryogenic callus protoplast + mesophyll protoplast sometimes results in the ...
Xiaodong CAI, Jing FU, Wenwu GUO
doaj   +1 more source

Characterization of Extracellular Vesicles Produced by Aspergillus fumigatus Protoplasts

mSphere, 2020
Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by ...
Juliana Rizzo, Thibault Chaze, Kildare Miranda, Robert W. Roberson, Olivier Gorgette, Leonardo Nimrichter, Mariette Matondo, Jean-Paul Latgé, Anne Beauvais, Marcio L. Rodrigues   +9 more
doaj   +3 more sources

Rapid Investigation of Functional Roles of Genes in Regulation of Leaf Senescence Using Arabidopsis Protoplasts

Frontiers in Plant Science, 2022
Leaf senescence is the final stage of leaf development preceding death, which involves a significant cellular metabolic transition from anabolism to catabolism.
Phan Phuong Thao Doan, Jin Hee Kim, Jeongsik Kim, Jeongsik Kim, Jeongsik Kim   +4 more
doaj   +1 more source

Effects of Movement Protein Mutations on the Formation of Tubules in Plant Protoplasts Expressing a Fusion Between the Green Fluorescent Protein and Cauliflower mosaic virus Movement Protein

Molecular Plant-Microbe Interactions, 2001
Fusions between the green fluorescent protein (GFP) and the Cauliflower mosaic virus (CaMV) movement protein (MP) induce the formation of fluorescent foci and surface tubules in Arabidopsis thaliana leaf mesophyll protoplasts.
Zhong Huang, Yu Han, Stephen H. Howell
doaj   +1 more source