Results 11 to 20 of about 58,172 (308)

Mitochondrial Genome of Callus Protoplast Has a Role in Mesophyll Protoplast Regeneration in Citrus: Evidence From Transgenic GFP Somatic Homo-Fusion

Horticultural Plant Journal, 2017
Protoplast fusion has great potential in citrus improvement. Although citrus mesophyll protoplasts usually cannot divide and regenerate, symmetric protoplast fusion of embryogenic callus protoplast + mesophyll protoplast sometimes results in the ...
Xiaodong CAI, Jing FU, Wenwu GUO
doaj   +1 more source

Optimized methods for random and targeted mutagenesis in field pea (Pisum sativum L.)

Frontiers in Plant Science, 2022
Field pea is an important pulse crop for its dense nutritional profile and contribution to sustainable agricultural practices. Recently, it has received extensive attention as a potential leading source of plant-based proteins.
Prashant Kumar Pandey, Pankaj Bhowmik, Sateesh Kagale   +2 more
doaj   +1 more source

Effects of Movement Protein Mutations on the Formation of Tubules in Plant Protoplasts Expressing a Fusion Between the Green Fluorescent Protein and Cauliflower mosaic virus Movement Protein

Molecular Plant-Microbe Interactions, 2001
Fusions between the green fluorescent protein (GFP) and the Cauliflower mosaic virus (CaMV) movement protein (MP) induce the formation of fluorescent foci and surface tubules in Arabidopsis thaliana leaf mesophyll protoplasts.
Zhong Huang, Yu Han, Stephen H. Howell
doaj   +1 more source

Rapid Investigation of Functional Roles of Genes in Regulation of Leaf Senescence Using Arabidopsis Protoplasts

Frontiers in Plant Science, 2022
Leaf senescence is the final stage of leaf development preceding death, which involves a significant cellular metabolic transition from anabolism to catabolism.
Phan Phuong Thao Doan, Jin Hee Kim, Jeongsik Kim, Jeongsik Kim, Jeongsik Kim   +4 more
doaj   +1 more source

The c-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi-mediated sorting to the vacuole [PDF]

, 2003
We have assessed the ability of the plant secretory pathway to handle the expression of complex heterologous proteins by investigating the fate of a hybrid immunoglobulin A/G in tobacco cells.
Hadlington, Jane L., Santoro, Aniello, Nuttall, James, Denecke, J. (Jurgen), Ma, Julian K.-C., Vitale, A. (Alessandro), Frigerio, Lorenzo   +6 more
core   +1 more source

Characterization of Extracellular Vesicles Produced by Aspergillus fumigatus Protoplasts

mSphere, 2020
Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by ...
Juliana Rizzo, Thibault Chaze, Kildare Miranda, Robert W. Roberson, Olivier Gorgette, Leonardo Nimrichter, Mariette Matondo, Jean-Paul Latgé, Anne Beauvais, Marcio L. Rodrigues   +9 more
doaj   +3 more sources

The isolation and regeneration of protoplasts from the mycorrhizal fungi Lactarius piperatus and Suillus variegatus

Acta Biologica Slovenica, 1989
An efficient system for the isolation and regeneration of mycelial protoplasts from the my­ corrhizal fungus Lactarius piperatus has been developed. The highest yield of protoplasts from the four-day cultures grown on solid media was 4x 107 protoplasts ...
Jana Zel, Marina Dermastia, Nada Gogala
doaj   +1 more source

The role of CDC48 in the retro-translocation of non-ubiquitinated toxin substrates in plant cells [PDF]

, 2009
When the catalytic A subunits of the castor bean toxins ricin and Ricinus communis agglutinin (denoted as RTA and RCA A, respectively) are delivered into the endoplasmic reticulum (ER) of tobacco protoplasts, they become substrates for ER ...
Richard S. Marshall, Nicholas A. Jolliffe, Aldo Ceriotti, Christopher J. Snowden, J. Michael Lord, Lorenzo Frigerio, Lynne M. Roberts, Vitale, Romisch, Meusser, Braun, Ye, Rabinovich, Jarosch, Ye, Rape, Lee, Worthington, Kothe, Feiler, Rancour, Muller, Di Cola, Di Cola, Tabe, Hellens, Frigerio, Pedrazzini, Hunter, Chaddock, Jolliffe, Roberts, Campbell, Maliga, Denecke, Klein, Martinez, Allen, Roberts, Wojcik, Dalal, Simpson, Day, Tsai, Bays, Denic, Carvalho, Swanson, Deak, Schuberth, Richly, Yoshida, Yoshida, Wiertz, Brandizzi, Roberts, Lord, Deeks, Wahlman, Wojcik, Carlson, Vashist, Vashist, Caldwell, Schmid, Kirst, Smalle, Carter, Contento, Pimpl   +69 more
core   +1 more source

A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability

MethodsX, 2015
Protoplasts have been widely used for genetic transformation, cell fusion, and somatic mutation due to the absence of a cell wall. However, without the protection of a cell wall, protoplasts are easy to rupture and aggregate during washing, collecting ...
Rong Lei, Wenjie Qiao, Fan Hu, Hongshan Jiang, Shuifang Zhu   +4 more
doaj   +1 more source

Strain Improvement and Strain Maintenance Revisited. The Use of Actinoplanes teichomyceticus ATCC 31121 Protoplasts in the Identification of Candidates for Enhanced Teicoplanin Production

Antibiotics, 2021
Multicellular cooperation in actinomycetes is a division of labor-based beneficial trait where phenotypically specialized clonal subpopulations, or genetically distinct lineages, perform complementary tasks.
Luca Mellere, Adriana Bava, Carmine Capozzoli, Paola Branduardi, Francesca Berini, Fabrizio Beltrametti   +5 more
doaj   +1 more source