Results 41 to 50 of about 38,339 (281)
PlantsThe epigenetic variation in protoplast regeneration is a topic that has attracted interest recently. To elucidate the role of DNA methylation in the regeneration of protoplasts from the ponkan (Citrus reticulata), this study employs the methylation ...
Lun Wang, Jiaojiao Zhang, Xiaoyong Xu
doaj +1 more source
TED1 Promotes the Assembly of SCFD3‐D14‐D53 to Reinforce Strigolactone Signaling in Rice
Advanced Science, EarlyView.This study identifies TED1 as a positive regulator of strigolactones (SL) signaling in rice. The ted1 mutants display dwarfism and increased tillering with significantly reduced SLs sensitivity. Through its longer C‐terminal region, TED1 interacts with D53 and D14 to promote the assembly of SCFD3‐D14‐D53, thereby facilitating the efficient ...
Sheng Luo +22 more
wiley +1 more source
Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts – isolation and fusion
Journal of Applied Botany and Food Quality, 2012 This paper reports on the isolation of haploid and mixoploid protoplasts in the genus Cucumis. The cucumber mixoploid plants (C. sativus L., 2x/4x; 2x = 14) were obtained after oryzalin treatments and the mesophyll protoplasts (2x/4x/8x) were isolated ...
Dagmar Skálová +4 more
doaj
Efficient transformation of oil palm protoplasts by PEG-mediated transfection and DNA microinjection. [PDF]
PLoS ONE, 2014 Genetic engineering remains a major challenge in oil palm (Elaeis guineensis) because particle bombardment and Agrobacterium-mediated transformation are laborious and/or inefficient in this species, often producing chimeric plants and escapes ...
Mat Yunus Abdul Masani +4 more
doaj +1 more source
A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants
Plants, 2022 In vivo localization of proteins using fluorescence-based approaches by fusion of the protein of interest (POI) to a fluorescent protein is a cost- and time-effective tool to gain insights into its physiological function in a plant cell.
Hagen Stellmach +4 more
doaj +1 more source
Preparation of protoplasts from Chlorella protothecoides
, 2012 This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested.
Lu, Y.(luyz@yahoo.com) +3 more
core +1 more source
Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation
Plants, 2021 Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant ...
Taras Pasternak +2 more
doaj +1 more source
Gymnoplasts instead of “Protoplasts” [PDF]
Nature, 1967 MICROBIOLOGISTS call yeast cells and bacteria devoid of their cell wall “protoplasts”1. According to a 100 yr old definition the protoplast represents the totality of the living cell constituents quite independent of whether the cell is coated by a wall or not.
openaire +2 more sources
Directed electrofusion between protoplasts with different responses in a mass fusion system
, 1985 Protoplasts of Solanum brevidens (leaves) and Nicotiana rustica (suspensions) have been aligned and fused electrically between widely spaced electrodes, and the yield of 1:1 (binary) fusion products in chains of aligned protoplasts has been determined by
Tempelaar, M. J. +3 more
core +1 more source
Фактори експериментальної еволюції організмів, 2015 Aims. In order to develop the background for the creation of novel carbon nanotubes (CNTs) based approaches in plant biotechnology the effect of non-covalently functionalized single-walled and multi-walled CNTs (SWCNTs and MWCNTs, respectively) on ...
О. М. Бурлака +3 more
doaj