Results 41 to 50 of about 39,210 (240)

Dual Repression by IPA1 Fine‐Tunes OsbZIP79‐Mediated Salt Tolerance in Rice

open access: yesAdvanced Science, EarlyView.
Dual repression by IPA1 fine‐tunes OsbZIP79‐mediated salt tolerance in rice: direct transcriptional inhibition under normal conditions and salt‐induced degradation under stress. This dual mechanism activates OsbZIP79 to regulate Na+/K+ homeostasis and redox balance via downstream genes, enabling optimal salt stress response.
Hui Wang   +12 more
wiley   +1 more source

Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts – isolation and fusion

open access: yesJournal of Applied Botany and Food Quality, 2012
This paper reports on the isolation of haploid and mixoploid protoplasts in the genus Cucumis. The cucumber mixoploid plants (C. sativus L., 2x/4x; 2x = 14) were obtained after oryzalin treatments and the mesophyll protoplasts (2x/4x/8x) were isolated ...
Dagmar Skálová   +4 more
doaj  

eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’

open access: yesPlants, 2023
Although CRISPR/Cas-based genome editing has been widely used for plant genetic engineering, its application in the genetic improvement of trees has been limited, partly because of challenges in Agrobacterium-mediated transformation.
Guoliang Yuan   +6 more
doaj   +1 more source

A Scalable Droplet Microfluidic Platform That Overcomes Large‐Cell Constraints for Long‐Term Plant Cell Culture and Embryogenesis Analysis

open access: yesAdvanced Science, EarlyView.
This work presents a large‐cell‐compatible droplet microfluidic platform that overcomes long‐standing barriers in plant cell culture by enabling high‐fidelity encapsulation, novel multilayer 3D droplet incubation for scalable long‐term culture, and support for the full developmental progression of large plant cells.
Arman Mirmiran   +10 more
wiley   +1 more source

Efficient transformation of oil palm protoplasts by PEG-mediated transfection and DNA microinjection. [PDF]

open access: yesPLoS ONE, 2014
Genetic engineering remains a major challenge in oil palm (Elaeis guineensis) because particle bombardment and Agrobacterium-mediated transformation are laborious and/or inefficient in this species, often producing chimeric plants and escapes ...
Mat Yunus Abdul Masani   +4 more
doaj   +1 more source

Blue Light Receptor PHR2‐Mediated Spectrum Antiviral Immunity

open access: yesAdvanced Science, EarlyView.
Blue light suppresses RBSDV through the blue‐light receptor ZmPHR2, which interacts with ZmISP in chloroplasts to trigger ROS burst and antiviral defense. The viral effector P6 enhances ZmPHR2–ZmISP interaction, alters their distribution, and suppresses ROS.
Runyi Chen   +17 more
wiley   +1 more source

Дослідження впливу вуглецевих нанотрубок на протопласти тютюну для створення нових підходів у біотехнології рослин

open access: yesФактори експериментальної еволюції організмів, 2015
Aims. In order to develop the background for the creation of novel carbon nanotubes (CNTs) based approaches in plant biotechnology the effect of non-covalently functionalized single-walled and multi-walled CNTs (SWCNTs and MWCNTs, respectively) on ...
О. М. Бурлака   +3 more
doaj  

A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants

open access: yesPlants, 2022
In vivo localization of proteins using fluorescence-based approaches by fusion of the protein of interest (POI) to a fluorescent protein is a cost- and time-effective tool to gain insights into its physiological function in a plant cell.
Hagen Stellmach   +4 more
doaj   +1 more source

Isolation, culture and regeneration of plants from potato protoplasts

open access: yes, 1989
A technique is described for the routine isolation of protoplasts from storage parenchyma cells of potato tubers grown in vitro. The protoplasts typically contained many starch grains.
Jones, M.G.K.   +5 more
core   +1 more source

Chrysanthemum Morifolium–Derived Nanovesicles Reduce Lactate‐Associated Histone Lactylation and Modulate Microglia‐Mediated Neuroinflammation in Sepsis‐Associated Encephalopathy

open access: yesAdvanced Science, EarlyView.
When used to treat sepsis‐associated encephalopathy, Chrysanthemum morifolium–derived nanovesicles can inhibit the H3K18la/NF‐κB axis to regulate microglia‐mediated neuroinflammation. ABSTRACT Sepsis‐associated encephalopathy (SAE)—a complication of the central nervous system in patients with sepsis—markedly diminishes cognitive functions and is linked
Kaiqiang Li   +11 more
wiley   +1 more source

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