Results 51 to 60 of about 38,339 (281)

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, EarlyView.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

Cryopreservation of rye protoplasts by vitrification [PDF]

Cryobiology, 1989
A procedure has been developed for the vitrification of mesophyll protoplasts isolated from leaves of nonacclimated (NA) and cold-acclimated (ACC) winter rye seedlings (Secale cereale L. cv Puma). The procedure involves (a) equilibration (loading) of the protoplasts with an intermediate concentration (1.5, 1.75, or 2.0 molar) of ethylene glycol (EG) at
R, Langis, P L, Steponkus
openaire   +2 more sources

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley

Frontiers in Genome Editing, 2021
The Eukaryotic Translation Initiation Factor 4E (EIF4E) is a well-known susceptibility factor for potyvirus infections in many plant species. The barley yellow mosaic virus disease, caused by the bymoviruses Barley yellow mosaic virus (BaYMV) and Barley ...
Robert Eric Hoffie, Ingrid Otto, Dragan Perovic, Nagaveni Budhagatapalli, Antje Habekuß, Frank Ordon, Jochen Kumlehn   +6 more
doaj   +1 more source

Viscosity of concentrated mRNA solutions: Implications for downstream processing

Biotechnology Progress, EarlyView.
Abstract Messenger RNA (mRNA) therapeutics can be used to protect against infectious disease and for the treatment of cancers and genetic disorders. In order to obtain the desired therapeutic dosage, it is necessary to concentrate the mRNA to levels where the viscosity of the mRNA solutions can become significant.
Amin Javidanbardan, Fatemeh Najafi, Ziqiao Wang, Andrew L. Zydney   +3 more
wiley   +1 more source

Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb

Journal of Applied Sciences and Environmental Management, 2013
Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
I Mukhtar, R Bajwa, G Nasim
doaj   +2 more sources

Fluorescence and scanning electron microscopy of citrus sinensis protoplasts

Evidência, 2008
The technology of protoplasts isolation is an important tool for studies of plasmatic membrane biochemistry and physiology and cell wall regeneration. Protoplasts of “Caipira” sweet orange (Citrus sinensis L.
Paulo Celso de Mello-Farias et al., Laudete Maria Sartoretto,, Ana Lúcia Soares Chaves   +2 more
doaj  

Independent leaf and root responses to autumn freezing in perennial herbs: Insights from cutting and transplant manipulations

Grassland Research, EarlyView.
In this study, we compared two sampling approaches to assess autumn freezing resistance. Our goal was to determine whether the commonly employed cutting method, which disrupts root‐tissue connectivity, provides a valid measure of true freezing resistance.
Zhengjie Yan, Cheng Chen, Xin Jing, Shuai Ren, Junpeng Rui, Tao Wang, Jin‐Sheng He   +6 more
wiley   +1 more source

Time saving method for protoplast isolation, transformation and transient gene expression assay in barley

Agricultural and Food Science, 1994
This study was conducted to establish a rapid method for barley (Hordeum vulgare L.) protoplast isolation to provide an easy-to-use procedure for the transformation and primary investigation of new gene constructs by transient gene expression assays ...
Barnabas Jenes, Matti Puolimatka, Pedro Bittencourt, Seppo Pulli   +3 more
doaj   +2 more sources

Subcellular Localization of Iron in Rhizophora mangle Leaves Revealed by Integrated Perls Reaction, TEM, STEM‐HAADF, and EDS Analyses

Microscopy Research and Technique, EarlyView.
Perls–TEM–STEM‐HAADF–EDS enables high‐resolution localization and elemental characterization of Fe‐containing deposits in Rhizophora mangle leaf cells. ABSTRACT Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in
Silvia Rodrigues Machado, Hiulana Pereira Arrivabene, Camilla Rozindo Dias Milanez, Margarida Juri Saeki, Yve Canaveze   +4 more
wiley   +1 more source