Results 51 to 60 of about 39,210 (240)
Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation
Plants, 2021 Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant ...
Taras Pasternak +2 more
doaj +1 more source
An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP-84. [PDF]
MicrobiologyopenThe study presents an optimized, reproducible protoplast transfection method for Geobacillus stearothermophilus. The method enables efficient uptake of native and fully synthetic TP‐84 bacteriophage genomes, their “rebooting” in vivo and production of infectious phage particles.
Sobolewski I +4 more
europepmc +2 more sources
Advanced Science, EarlyView.An integrated phenomics–genomics framework captures dynamic salt‐stress responses in laboratory‐grown cotton seedlings through time‐series hyperspectral imaging and non‐destructive i‐trait extraction. Hierarchical screening identifies biologically relevant, heritable, and non‐redundant traits for high‐resolution GWAS, identifying GhST7.
Bowei Xu +11 more
wiley +1 more source
Agricultural and Food Science, 1994 This study was conducted to establish a rapid method for barley (Hordeum vulgare L.) protoplast isolation to provide an easy-to-use procedure for the transformation and primary investigation of new gene constructs by transient gene expression assays ...
Barnabas Jenes +3 more
doaj +2 more sources
Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb
Journal of Applied Sciences and Environmental Management, 2013 Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
I Mukhtar, R Bajwa, G Nasim
doaj +2 more sources
Isolation and Fusion of Protoplasts from Basella rubra Leaf and Stem Cultures [PDF]
, 1999 Isolation and fusion of protoplasts from Basella rubra leaf and stem were examined. In preparation of protoplasts, the enzyme solution gave a high yield of protoplasts. The diameter of protoplasts induced ranged from 30μm to 120μm.
Miyashige, Kayoko +6 more
core +1 more source
Induction and Enhancement of Bacteriocin Production (Review)
Biotechnology and Bioengineering, EarlyView.ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley +1 more source
Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry
Cytometry Part A, EarlyView.ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian +3 more
wiley +1 more source
Immobilization of Plant Protoplasts: Viability Studies
, 1985 Protoplasts of Daucus carota Ca68 and Catharanthusroseus have been inmobilized by entrapment in gelformingpolysaccharides (kappa-carrageenan, agarose andalginate).
Linsefors, L, Brodelius, Peter,
core +2 more sources
Microscopy Research and Technique, EarlyView.Perls–TEM–STEM‐HAADF–EDS enables high‐resolution localization and elemental characterization of Fe‐containing deposits in Rhizophora mangle leaf cells. ABSTRACT Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in
Silvia Rodrigues Machado +4 more
wiley +1 more source