Efficient transformation of oil palm protoplasts by PEG-mediated transfection and DNA microinjection. [PDF]
PLoS ONE, 2014 Genetic engineering remains a major challenge in oil palm (Elaeis guineensis) because particle bombardment and Agrobacterium-mediated transformation are laborious and/or inefficient in this species, often producing chimeric plants and escapes ...
Mat Yunus Abdul Masani +4 more
doaj +1 more source
Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote [PDF]
, 2017 In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. Unlike animals, plant zygotes are transcriptionally active, implying that plants have evolved specific mechanisms to control transcriptional activation of ...
Aichinger, Ernst +9 more
core +2 more sources
Gymnoplasts instead of “Protoplasts” [PDF]
Nature, 1967 MICROBIOLOGISTS call yeast cells and bacteria devoid of their cell wall “protoplasts”1. According to a 100 yr old definition the protoplast represents the totality of the living cell constituents quite independent of whether the cell is coated by a wall or not.
openaire +2 more sources
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
Advanced Science, EarlyView.A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts – isolation and fusion
Journal of Applied Botany and Food Quality, 2012 This paper reports on the isolation of haploid and mixoploid protoplasts in the genus Cucumis. The cucumber mixoploid plants (C. sativus L., 2x/4x; 2x = 14) were obtained after oryzalin treatments and the mesophyll protoplasts (2x/4x/8x) were isolated ...
Dagmar Skálová +4 more
doaj
A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants
Plants, 2022 In vivo localization of proteins using fluorescence-based approaches by fusion of the protein of interest (POI) to a fluorescent protein is a cost- and time-effective tool to gain insights into its physiological function in a plant cell.
Hagen Stellmach +4 more
doaj +1 more source
Development of an efficient transformation system for Dothistromin pini : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University [PDF]
, 1996 A transformation system has been developed for the plant pathogenic fungus Dothistroma pini using a positive selection system based on the Escherichia coli hygromycin B phosphotransferase gene (hph).
Bidlake, Anita Therese
core
Advanced Science, EarlyView.RNA structural profiling of Turnip Yellow Mosaic Virus by DMS‐MaPseq and DREEM analyses uncover that viral genome‐wide RSS is highly complicated and heterogeneous, with alternative RSSs widely distributed across the genome. Notably, the viral 3’ tRNA‐like structure adopts alternative conformations in vivo.
Jiaying Zhu +7 more
wiley +1 more source
Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation
Plants, 2021 Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant ...
Taras Pasternak +2 more
doaj +1 more source
MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response. [PDF]
, 2020 Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered ...
Ceciliato, Paulo HO +10 more
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