Transport engineering as a strategy to realize rapeseed's potential as a protein‐rich food
Pest Management Science, EarlyView.The heavily pesticide‐dependent rapeseed is an under‐utilized protein source for food owing to anti‐nutritional glucosinolates. Transport engineering technology may reduce glucosinolates in seeds while increasing defense in vegetative parts. Abstract Rapeseed is the world's second‐largest oilseed crop, and the low‐value press cake that remains after ...
Jakob Skytte Thorsen +2 more
wiley +1 more source
Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins. [PDF]
, 2013 Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene ...
Bae, Hanhong +10 more
core +2 more sources
Pest Management Science, EarlyView.A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos +3 more
wiley +1 more source
CRISPR-TSKO : a technique for efficient mutagenesis in specific cell types, tissues, or organs in Arabidopsis [PDF]
, 2019 Detailed functional analyses of many fundamentally important plant genes via conventional loss-of-function approaches are impeded by the severe pleiotropic phenotypes resulting from these losses.
Andrade Buono, Rafael +9 more
core +1 more source
Responsive Materials, EarlyView.An oxygen‐responsive living bioreactor was engineered via interspecies protoplast fusion, converting oxygen from an inhibitory stress into a programmable signal for sustained indole‐3‐propionic acid (IPA) production. This genetically encoded bioreactor enables robust aerobic operation and therapeutic efficacy of deoxynivalenol detoxification in vivo ...
Ning Ma +8 more
wiley +1 more source
Hevea brasiliensis seeds: A novel sustainable feedstock for ethanol production
Vietnam Journal of Chemistry, EarlyView.Abstract The rubber tree (Hevea brasiliensis) is cultivated on a large scale mainly for the extraction of latex in Brazil and Southeast Asia, also generating large quantities of seeds. These seeds currently do not have large‐scale applications and may thus be considered as a residue.
Giovano Tochetto +4 more
wiley +1 more source
Characterization of the extracellular lipase of Bacillus subtilis and its relationship to a membrane-bound lipase found in a mutant strain [PDF]
, 1979 Bacillus subtilis CMK33 is a mutant that is more osmotically fragile than the wild type when it is converted to the protoplast form. The protoplasts of this mutant contain a membrane-bound lipase, which is not found in protoplasts of the wild type ...
Kennedy, Mary B., Lennarz, W. J.
core
Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene [PDF]
, 2012 Background : The phytohormone ethylene is involved in a wide range of developmental processes and in mediating plant responses to biotic and abiotic stresses.
Bouzayen, Mondher +10 more
core +5 more sources
Journal of Integrative Plant Biology, EarlyView.Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang +7 more
wiley +1 more source
Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato [PDF]
, 2008 Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal ...
Bouzayen, Mondher +8 more
core +2 more sources