Results 61 to 70 of about 39,210 (240)
Salt‐induced nutritional and metabolic shifts in halophytes: implications for food security
Journal of the Science of Food and Agriculture, EarlyView.Abstract Plant species vary in their response to salinity: some crops show a degree of salt tolerance, while halophytes – whether wild or cultivated – are characterized by a high capacity to thrive under saline conditions. Halophytes are considered a source of valuable secondary metabolites with potential economic value, yet they might also produce ...
Giulia Atzori +9 more
wiley +1 more source
Preparation of protoplasts from Chlorella protothecoides
, 2012 This paper describes an enzymatic method for yielding protoplasts from the microalga Chlorella protothecoides. Four kinds of commercially available enzymes were tested.
Lu, Y.(luyz@yahoo.com) +3 more
core +1 more source
Liquid Phase Backscattered Scanning Electron Microscopy of Bacillus subtilis Spores
Small Methods, EarlyView.Graphene encapsulation enables liquid‐phase BSE‐SEM to visualize subsurface architecture of whole, hydrated Bacillus subtilis spores without staining. This approach is applied to visualize dormant spores and subsequent structural changes during spore germination, while imaging across a range of beam energies and Monte Carlo simulations provide a ...
Jack Bromley +8 more
wiley +1 more source
Assessing Biocide Effects on Protoplasts of Wood Decay Fungi [PDF]
Hyphal fragments and protoplasts of two wood-degrading fungi, Postia placenta and Trametes versicolor, were used to examine the effects of two fungicides, copper sulfate and azaconazole, on fungal health (as measured by oxygen consumption, glucose ...
Rui, Chen, Morrell, Jeffrey J.
core +4 more sources
Frontiers in MicrobiologyClubroot, caused by the obligate Chromist pathogen Plasmodiophora brassicae, is an important disease of brassica crops but little is known about its reproductive biology.
Afsaneh Sedaghatkish +3 more
doaj +1 more source
Vacuole development in evacuolated oat mesophyll protoplasts
, 1994 Protoplasts were prepared from oat leaf mesophyll cells and subsequently evacuolated by high speed centrifugation through a continuous Percoll gradient.
Newell, Jane Marie, Newell, J. M.
core
Yeast, EarlyView.Yeast transformations are mechanistically poorly understood and could be made orders of magnitude more efficient by three approaches. (1) Investigate mechanisms of DNA uptake including DNA phase separation. (2) Account for growth state. (3) Use systematic statistical design of experiments to test combinations of factors that enhance transformation ...
Alistair Elfick, Edward W. J. Wallace
wiley +1 more source
Isolation and culture of Celosia cristata L cell suspension protoplasts
Journal of Biological Researches, 2003 Developmental competence of Celosia cristata L. cell suspension-derived protoplasts was investigated. The protoplasts were isolatedfrom 3- to 9-d old cultures in enzyme solution containing 2% (w/v) Cellulase YC and 0.5% (w/v) Macerozyme R-10 which was ...
Retno Mastuti +4 more
doaj +1 more source
ENDO2 is modified in protoplasts.
, 2017 (A) N1 and N2 nucleases are the product of the ENDO2 gene. In gel nuclease assay of proteins extracted from protoplasts derived from WT leaves (WT Col), endo2 mutant leaves and from endo2 protoplasts transformed with pUC19-S35-ENDO2-His.
Narendra Singh Yadav (593707) +3 more
core +1 more source
A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis
Yeast, EarlyView.ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley +1 more source