Results 81 to 90 of about 38,339 (281)

ENDO2 is modified in protoplasts.

, 2017
(A) N1 and N2 nucleases are the product of the ENDO2 gene. In gel nuclease assay of proteins extracted from protoplasts derived from WT leaves (WT Col), endo2 mutant leaves and from endo2 protoplasts transformed with pUC19-S35-ENDO2-His.
Narendra Singh Yadav (593707), Gideon Grafi (3626255), Yemima Givaty-Rapp (3626252), Asif Khan (1740271)   +3 more
core   +1 more source

Natural variation in the GNH1 promoter enhances plant height and grain yield in rice

Journal of Integrative Plant Biology, EarlyView.
Natural variation in the GRAIN NUMBER AND PLANT HEIGHT 1 (GNH1) promoter alleviates repression by the C2H2‐type transcription factor ZFP36, upregulating GNH1 expression and promoting auxin accumulation, thereby increasing rice plant height and grain yield. This elite allele serves as a valuable genetic resource for molecular breeding of high‐yield rice.
Hongwei Xing, Huan Wang, Sheng Wu, Yuanjie Li, Kun Zhang, Chuanqing Sun, Hongying Sun   +6 more
wiley   +1 more source

Advances in Quercus ilex L. breeding: the CRISPR/Cas9 technology via ribonucleoproteins

Frontiers in Plant Science
The CRISPR/Cas9 ribonucleoprotein (RNP)-mediated technology represents a fascinating tool for modifying gene expression or mutagenesis as this system allows for obtaining transgene-free plants, avoiding exogenous DNA integration.
Vera Pavese, Andrea Moglia, Anna Maria Milani, Lorenzo Antonio Marino, Maria Teresa Martinez, Daniela Torello Marinoni, Roberto Botta, Elena Corredoira   +7 more
doaj   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

The permeability of electroporated cells and protoplasts of sugar beet.

, 1987
A simple method has been developed to determine the changes in permeability of protoplasts and intact cells when electroporated. Cells and protoplasts of sugar beet, Beta vulgaris L., were subjected to electric pulse treatments of different field ...
Jones, M.G.K., Jones, M. G. K., Lindsey, K.   +2 more
core  

TaHDA1‐mediated histone and non‐histone deacetylation orchestrates drought tolerance in wheat

Journal of Integrative Plant Biology, EarlyView.
The histone deacetylase TaHDA1 is a key regulator linking histone and non‐histone deacetylation with drought adaptation and a potential target for improving drought resilience and grain nutritional quality in wheat. ABSTRACT Drought stress severely constrains wheat (Triticum aestivum L.) growth and productivity.
Jinpeng Li, Danyang Zhao, Xiao Peng, Xinran Wu, Chenji Zhang, Huitao An, Wei Chu, Jingchen Lin, Zehui Liu, Qun Yang, Debiao Liu, Xiaoyu Liu, Wanghongan Jia, Yi Pei, Mingming Xin, Yingyin Yao, Weilong Guo, Huiru Peng, Zhongfu Ni, Qixin Sun, Xingbei Liu, Zhaorong Hu   +21 more
wiley   +1 more source

A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Plants
Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants.
Aristotelis Azariadis, Olga A. Andrzejczak, Frida M. Carlsen, Ida Westberg, Henrik Brinch-Pedersen, Bent L. Petersen, Kim H. Hebelstrup   +6 more
doaj   +1 more source

Isolation and Fusion of Cotton Protoplasts [PDF]

, 2001
Protoplasts were isolated from five species of Gossypium. Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated
Hartwig, Ronald C., Smith, Roberta H., Renfroe, Michael H.   +2 more
core   +1 more source

Plant cis‐regulatory grammar: Decoding the multidimensional code of transcriptional regulation for programmable crop engineering

Journal of Integrative Plant Biology, EarlyView.
This review decodes the grammar of plant cis‐regulatory elements, revealing that their function emerges from sequence, chromatin accessibility, 3D topology, and cell type context. It highlights how single‐cell omics, CRISPR dissection, and AI‐driven design converge to enable predictive engineering and crop improvement. ABSTRACT Cis‐regulatory elements (
Libin Zhang, Maoteng Li
wiley   +1 more source

Isolation and characterisation of mutants of cowpea mosaic virus [PDF]

A nitrous acid-induced, temperature sensitive mutant of cowpea mosaic virus (CPMV) known as 8-14, (Evans 1985, Virology 1985, 141, 275-282), was characterised.
Holness, Claire Louise Lesley
core