Results 81 to 90 of about 39,210 (240)
Electroporation of Small RNAs into Plant Protoplasts
To study tRNA import into plant mitochondria, we have set up a system to follow the fatein vivoof tRNA transcripts introduced into plant protoplasts by electroporation. Conditions were optimized for maximum tRNA uptake into potato protoplasts.
Wintz, H. (Henri) +3 more
core +1 more source
Isolation and culture of protoplasts of Côte d'Ivoire’s pearl millet (Pennisetum glaucum (L) R) varieties [PDF]
Objective: Protoplasts are the ideal material for genetic transformation of plants. This requires that the protoplasts have the ability to regenerate whole plants. The objective of this study is to isolate protoplasts from cell suspensions and test their
Kouakou, T +5 more
core +1 more source
Natural variation in the GNH1 promoter enhances plant height and grain yield in rice
Natural variation in the GRAIN NUMBER AND PLANT HEIGHT 1 (GNH1) promoter alleviates repression by the C2H2‐type transcription factor ZFP36, upregulating GNH1 expression and promoting auxin accumulation, thereby increasing rice plant height and grain yield. This elite allele serves as a valuable genetic resource for molecular breeding of high‐yield rice.
Hongwei Xing +6 more
wiley +1 more source
Transient gene expression in electroporated protoplasts and intact cells of sugar beet.
Factors influencing the transient expression of introduced foreign DNA in electroporated protoplasts and intact cells of sugar beet were determined by assaying for the activity of chloramphenicol acetyltransferase (CAT), using a rectangular pulse ...
Jones, M.G.K. +2 more
core
TaHDA1‐mediated histone and non‐histone deacetylation orchestrates drought tolerance in wheat
The histone deacetylase TaHDA1 is a key regulator linking histone and non‐histone deacetylation with drought adaptation and a potential target for improving drought resilience and grain nutritional quality in wheat. ABSTRACT Drought stress severely constrains wheat (Triticum aestivum L.) growth and productivity.
Jinpeng Li +21 more
wiley +1 more source
Isolation and characterisation of mutants of cowpea mosaic virus [PDF]
A nitrous acid-induced, temperature sensitive mutant of cowpea mosaic virus (CPMV) known as 8-14, (Evans 1985, Virology 1985, 141, 275-282), was characterised.
Holness, Claire Louise Lesley
core
This review decodes the grammar of plant cis‐regulatory elements, revealing that their function emerges from sequence, chromatin accessibility, 3D topology, and cell type context. It highlights how single‐cell omics, CRISPR dissection, and AI‐driven design converge to enable predictive engineering and crop improvement. ABSTRACT Cis‐regulatory elements (
Libin Zhang, Maoteng Li
wiley +1 more source
To induce drought resistance, callus from leaf discs and protoplast of Pelargonium x hortorum (‘Deep Salmon’ and ‘Panaché Sud’) were grown in vitro in osmoticum rich medium.
M. MOKHTARI, N. DORION, K. RAMDANI
doaj
Advances in Quercus ilex L. breeding: the CRISPR/Cas9 technology via ribonucleoproteins
The CRISPR/Cas9 ribonucleoprotein (RNP)-mediated technology represents a fascinating tool for modifying gene expression or mutagenesis as this system allows for obtaining transgene-free plants, avoiding exogenous DNA integration.
Vera Pavese +7 more
doaj +1 more source
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova +11 more
doaj +1 more source

