Results 81 to 90 of about 39,210 (240)

Electroporation of Small RNAs into Plant Protoplasts

open access: yes, 1996
To study tRNA import into plant mitochondria, we have set up a system to follow the fatein vivoof tRNA transcripts introduced into plant protoplasts by electroporation. Conditions were optimized for maximum tRNA uptake into potato protoplasts.
Wintz, H. (Henri)   +3 more
core   +1 more source

Isolation and culture of protoplasts of Côte d'Ivoire’s pearl millet (Pennisetum glaucum (L) R) varieties [PDF]

open access: yes, 2015
Objective: Protoplasts are the ideal material for genetic transformation of plants. This requires that the protoplasts have the ability to regenerate whole plants. The objective of this study is to isolate protoplasts from cell suspensions and test their
Kouakou, T   +5 more
core   +1 more source

Natural variation in the GNH1 promoter enhances plant height and grain yield in rice

open access: yesJournal of Integrative Plant Biology, EarlyView.
Natural variation in the GRAIN NUMBER AND PLANT HEIGHT 1 (GNH1) promoter alleviates repression by the C2H2‐type transcription factor ZFP36, upregulating GNH1 expression and promoting auxin accumulation, thereby increasing rice plant height and grain yield. This elite allele serves as a valuable genetic resource for molecular breeding of high‐yield rice.
Hongwei Xing   +6 more
wiley   +1 more source

Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

open access: yes, 1987
Factors influencing the transient expression of introduced foreign DNA in electroporated protoplasts and intact cells of sugar beet were determined by assaying for the activity of chloramphenicol acetyltransferase (CAT), using a rectangular pulse ...
Jones, M.G.K.   +2 more
core  

TaHDA1‐mediated histone and non‐histone deacetylation orchestrates drought tolerance in wheat

open access: yesJournal of Integrative Plant Biology, EarlyView.
The histone deacetylase TaHDA1 is a key regulator linking histone and non‐histone deacetylation with drought adaptation and a potential target for improving drought resilience and grain nutritional quality in wheat. ABSTRACT Drought stress severely constrains wheat (Triticum aestivum L.) growth and productivity.
Jinpeng Li   +21 more
wiley   +1 more source

Isolation and characterisation of mutants of cowpea mosaic virus [PDF]

open access: yes
A nitrous acid-induced, temperature sensitive mutant of cowpea mosaic virus (CPMV) known as 8-14, (Evans 1985, Virology 1985, 141, 275-282), was characterised.
Holness, Claire Louise Lesley
core  

Plant cis‐regulatory grammar: Decoding the multidimensional code of transcriptional regulation for programmable crop engineering

open access: yesJournal of Integrative Plant Biology, EarlyView.
This review decodes the grammar of plant cis‐regulatory elements, revealing that their function emerges from sequence, chromatin accessibility, 3D topology, and cell type context. It highlights how single‐cell omics, CRISPR dissection, and AI‐driven design converge to enable predictive engineering and crop improvement. ABSTRACT Cis‐regulatory elements (
Libin Zhang, Maoteng Li
wiley   +1 more source

Conditions de sélection in vitro de cals issus des disques foliaires et des protoplastes de Pelargonium tolérant plus la sécheresse

open access: yesRevue Marocaine des Sciences Agronomiques et Vétérinaires, 2015
To induce drought resistance, callus from leaf discs and protoplast of Pelargonium x hortorum (‘Deep Salmon’ and ‘Panaché Sud’) were grown in vitro in osmoticum rich medium.
M. MOKHTARI, N. DORION, K. RAMDANI
doaj  

Advances in Quercus ilex L. breeding: the CRISPR/Cas9 technology via ribonucleoproteins

open access: yesFrontiers in Plant Science
The CRISPR/Cas9 ribonucleoprotein (RNP)-mediated technology represents a fascinating tool for modifying gene expression or mutagenesis as this system allows for obtaining transgene-free plants, avoiding exogenous DNA integration.
Vera Pavese   +7 more
doaj   +1 more source

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

open access: yesВавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova   +11 more
doaj   +1 more source

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