Results 101 to 110 of about 58,172 (308)

Advances in Quercus ilex L. breeding: the CRISPR/Cas9 technology via ribonucleoproteins

Frontiers in Plant Science
The CRISPR/Cas9 ribonucleoprotein (RNP)-mediated technology represents a fascinating tool for modifying gene expression or mutagenesis as this system allows for obtaining transgene-free plants, avoiding exogenous DNA integration.
Vera Pavese, Andrea Moglia, Anna Maria Milani, Lorenzo Antonio Marino, Maria Teresa Martinez, Daniela Torello Marinoni, Roberto Botta, Elena Corredoira   +7 more
doaj   +1 more source

GAME9 regulates the biosynthesis of steroidal alkaloids and upstream isoprenoids in the plant mevalonate pathway [PDF]

, 2016
Steroidal glycoalkaloids (SGAs) are cholesterol-derived molecules produced by solanaceous species. They contribute to pathogen defence but are toxic to humans and considered as anti-nutritional compounds.
Aharoni, Asaph, Burdman, Saul, Cárdenas, Pablo D, Dewangan, Veena, Giri, Ashok P, Goossens, Alain, Malitsky, Sergey, Meir, Sagit, Pollier, Jacob, Rogachev, Ilana, Sonawane, Prashant D, Tal, Lior, Vanden Bossche, Robin, Weithorn, Efrat   +13 more
core   +1 more source

SlGRF1 mediates gibberellin signaling to control cut‐budding in tomato

Journal of Integrative Plant Biology, EarlyView.
Upon wounding, cytokinin increases to initiate callus formation. Gibberellin enhances this, but later blocks bud formation by suppressing SlGRF1. Wounding‐induced protein SlGRF1 triggers buds via NAM1, EPF4, and ER2. Hormonal shifts control cut‐budding phases.
Yaping Xu, Yinhuan Xie, Huimin Jia, Qianqian Li, Xinqin Liu, Ting Ma, Zhaobo Lang, Qingfeng Niu   +7 more
wiley   +1 more source

A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Plants
Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants.
Aristotelis Azariadis, Olga A. Andrzejczak, Frida M. Carlsen, Ida Westberg, Henrik Brinch-Pedersen, Bent L. Petersen, Kim H. Hebelstrup   +6 more
doaj   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, EarlyView.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review [PDF]

Horticultural Science, 2004
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-ing.
openaire   +2 more sources

Constitution and function of intergenic genome regions in cultivated versus annual wild soybean in China

Journal of Integrative Plant Biology, EarlyView.
Three‐case restricted two‐stage multi‐locus multi‐allele genome‐wide association study identified main‐effect genes and detected the regulatory effects of intergenic loci on genes, enabling comprehensive dissection of the genic‐intergenic genetic system for quantitative traits and providing a strategy for studying their genetic basis.
Xiaoshuai Hao, Xinyang Hu, Yongpeng Pan, Qi Zhang, Ran Ou, Fenfen Jiang, Fangdong Liu, Jiaoping Zhang, Wubin Wang, Guangnan Xing, Junyi Gai, Jianbo He   +11 more
wiley   +1 more source

Improved technique for isolation and culture of protoplasts from young leaves of mangosteen (Garcinia mangostana L.) [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2002
Improved technique for isolation of protoplasts from young leaves of mangosteen was developed using dark treatment and varying ages of in vitro-grown leaves. In this experiment different kinds and concentrations of cellulase Onozuka R-10, macerozyme R-10
Moosikapala, L., Te-chato, S.
doaj  

protoplast

, 2014
Citation: 'protoplast' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.P04917 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire   +1 more source

Transcriptional adapter ADA2 regulates yield and end‐use quality through liquid–liquid phase separation in wheat

Journal of Integrative Plant Biology, EarlyView.
The transcriptional adapter ADA2 interacts with the histone acetyltransferase GCN5 to regulate starch and storage protein content in wheat grains through histone acetylation. ADA2 undergoes phase separation and is modulated by GCN5, affecting histone acetyltransferase activity and gene transcription.
Xiaobang Zhang, Ruijie Zhang, Yuhan Zhang, Tao Yang, Longfei Wu, Yuan Cao, Jun Xiao, Jigang Li, Xin Gao, Xinyou Cao, Jie Liu, Mingming Xin, Yuan Zong, Zhongfu Ni, Qixin Sun, Yingyin Yao   +15 more
wiley   +1 more source