Results 111 to 120 of about 39,210 (240)

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

Single‐Cell Transcriptomics Reveals the FA9–VAP Module Regulating Fatty Acid Accumulation in Soybean Seeds

Plant Biotechnology Journal, EarlyView.
ABSTRACT As a major commercial legume crop, soybean ranks among the world's most significant sources of edible oil and plant protein. We previously identified a SEIPIN homologue (FA9) at the fatty acid 9 locus that promotes fatty acid accumulation in soybean.
Hui Li, Tianshu Li, Xiaorui Xu, Jinhang Cui, Xin Chen, Jingyi Huang, Jia Song, Cui Mu, Xinyu Hong, Chunyan Liu, Ning Wang, Xue Han, Sui Wang, Qingshan Chen, Zhaoming Qi   +14 more
wiley   +1 more source

An Improved Approach to Protoplast Regeneration and Transfection in Banana (Musa acuminata AAA cv. Williams)

Applied Biosciences
Protoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo, Jean-Yves Paul, Anthony James, Rob Harding, James Dale   +4 more
doaj   +1 more source

HvPHO1 Controls a Glucose 1‐Phosphate–SnRK1 Signalling Module That Coordinates Starch Accumulation in Barley Endosperm

Plant Biotechnology Journal, EarlyView.
ABSTRACT Developing barley grains must balance the use of imported sucrose between soluble sugars and storage starch, but how this balance is controlled remains unclear. Here we show that loss of the plastidial α‐glucan phosphorylase HvPHO1 causes starch and soluble sugars to accumulate concurrently in barley grains.
Yulong Li, Yujia Chen, Jing Liu, Ping Yang, Guangqi Gao, Carlos Guzmán, Qiang Xu, Yazhou Zhang, Yanlin Liu, Pengfei Qi, Mei Deng, Shoufen Dai, Jian Ma, Guoyue Chen, Jirui Wang, Youliang Zheng, Yuming Wei, Qiantao Jiang   +17 more
wiley   +1 more source

Protoplast regeneration

California Agriculture, 1982
Not available – first paragraph follows: Plant cells without walls (protoplasts) can be isolated from leaves by a process of enzy-matically digesting away the middle lamellae between cells and the cell walls.
D Engler, R Grogan
doaj  

Relationship between Respiration and Photosynthesis in

, 1990
A mass spectrometric method combining 160/18O and 12C/13C isotopes was used to quantify the unidirectional fluxes of 02 and CO2 during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L.
Commelina Communis L, Guard Cell, Mesophyll Cell Protoplasts Of   +2 more
core  

Lessons From CABBI: Advancing Vegetative Oil Production in C4 Crops Through a Vertically Integrated DBTL Framework

Plant Biotechnology Journal, EarlyView.
ABSTRACT The global demand for renewable liquid fuels and biobased chemicals has intensified interest in engineering high‐biomass C4 grasses to accumulate triacylglycerol (TAG) in vegetative tissues. Achieving this goal requires coordinated solutions across molecular biology, crop science, chemical engineering, and life cycle assessment, disciplines ...
Daniel Lunn, Kiyoul Park, Narendra Naik Deshavath, Jia Dong, Kankshita Swaminathan, Jeremy Guest, Andrew D. B. Leakey, Nathaniel M. Butler, Jorg Schwender, Vijay Singh, John Shanklin, Fredy Altpeter, Edgar B. Cahoon   +12 more
wiley   +1 more source

Unravelling the Potential of Fungal Division of Labour in the Laccase Producer Coriolopsis trogii MUT3379 Through Protoplast Formation and Regeneration

Journal of Fungi
The white-rot fungus Coriolopsis trogii MUT3379 produces Lac3379-1 laccase at high yields due to the previous development of a robust fermentation process.
Luca Mellere, Adriana Bava, Jean Armengaud, Francesca Berini, Flavia Marinelli, Giovanna Cristina Varese, Federica Spina, Fabrizio Beltrametti   +7 more
doaj   +1 more source

A Method for Isolation of Protoplasts from Dermatophytes

, 1984
A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO, has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell
Srikantha, T, Rao, G Ramananda
core  

A Heat‐Inducible Rubisco Activase Safeguards Photosynthesis via Multilevel Regulation and Dual Function

Plant Biotechnology Journal, EarlyView.
ABSTRACT Rubisco activase (RCA) is critical for maintaining photosynthetic efficiency under heat stress, yet its regulatory mechanisms and functional diversification remain poorly understood. Here, we characterize a novel heat‐inducible RCA family member (RCA1), which plays a pivotal role in mediating multidimensional thermotolerance in a tropical ...
Yuqing Cao, Xiaomian Zhang, Fangqian Qu, Mengxuan Liu, Zheng Li, Bing Liu, Xianbin Gu, Qianyi Hu, Hong Zhou, Liangsheng Zhang, Yiping Xia, Xiuyun Wang   +11 more
wiley   +1 more source