Results 91 to 100 of about 39,210 (240)

An OsSK12/13‐OsERF60‐OsHsfA2c module regulates heat stress tolerance in rice

Journal of Integrative Plant Biology, EarlyView.
The heat‐induced GSK3‐like kinases OsSK12 and OsSK13 phosphorylate and stabilize the transcription factor OsERF60, which activates the expression of downstream genes including the heat shock factor gene OsHsfA2c. This regulatory module governs heat‐responsive gene induction, representing a mechanistic target for enhancing crop resilience under climate ...
Juan Gao, Zi‐Wei Yao, Zhi‐Ping Deng, Jing‐Jing Wang, Dong‐Jie Zhu, Dai‐Xi Yue, Hai‐Ping Lu, Jian‐Xiang Liu   +7 more
wiley   +1 more source

A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Plants
Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. bulbocastanum protoplasts and regeneration of gene-edited plants.
Aristotelis Azariadis, Olga A. Andrzejczak, Frida M. Carlsen, Ida Westberg, Henrik Brinch-Pedersen, Bent L. Petersen, Kim H. Hebelstrup   +6 more
doaj   +1 more source

The permeability of electroporated cells and protoplasts of sugar beet.

, 1987
A simple method has been developed to determine the changes in permeability of protoplasts and intact cells when electroporated. Cells and protoplasts of sugar beet, Beta vulgaris L., were subjected to electric pulse treatments of different field ...
Jones, M.G.K., Jones, M. G. K., Lindsey, K.   +2 more
core  

TaANT11‐4A enhances grain yield by orchestrating photosynthesis and carbohydrate metabolism through direct transcriptional activation

Journal of Integrative Plant Biology, EarlyView.
TaANT11‐4A is a positive regulator of wheat grain size and yield, directly activating TaEGY2, TaAAE1, and TaAAE2 to enhance photosynthesis and carbohydrate metabolism. The favorable Hap2 haplotype increases TaANT11‐4A expression and grain size, providing a genetic resource for breeding higher‐yielding wheat varieties.
Pengpeng Zhang, Jingjing Wen, Shan Gao, Jiajun Ma, Jiahui Jin, Xiaofei Ma, Wen‐Ting Liu, Juan Li, De‐Jun Han, Yue‐Qiang Zhang, Kun‐Ming Chen   +10 more
wiley   +1 more source

Disruption of OsMAPKKK24 activation by the Xanthomonas oryzae effector XopY leads to compromised disease resistance in rice

Journal of Integrative Plant Biology, EarlyView.
The kinase OsRLCK55 phosphorylates OsMAPKKK24 to positively regulate rice resistance against Xanthomonas oryzae. To compromise this defense, the effector XopY targets OsMAPKKK24 and suppresses its autophosphorylation and the transphosphorylation mediated by OsRLCK55, ultimately leading to reduced disease resistance.
Lilan Chen, Yongming Chen, Wencai Xu, Xiaofang Wu, Shanshan Deng, Zhanchun Wang, Dingzhong Tang, Wei Wang   +7 more
wiley   +1 more source

Visualising Physcomitrium patens protoplasts with full‐rotation soft X‐ray tomography

Journal of Microscopy, EarlyView.
Abstract Soft X‐ray tomography (SXT) is a label‐free, high‐resolution imaging modality that is optimal for 3D visualisation of whole hydrated cells. Although SXT has been extensively used to study remodelling in more than 130 mammalian cell lines, its application to plants remains largely unexplored. In this study, we present the first approach at full‐
Fariha Mahzabin Annesha, Sadia S. Tamanna, Katharina Grasser, Stefanie J. Müller‐Schüssele, Venera Weinhardt   +4 more
wiley   +1 more source

Improved technique for isolation and culture of protoplasts from young leaves of mangosteen (Garcinia mangostana L.) [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2002
Improved technique for isolation of protoplasts from young leaves of mangosteen was developed using dark treatment and varying ages of in vitro-grown leaves. In this experiment different kinds and concentrations of cellulase Onozuka R-10, macerozyme R-10
Moosikapala, L., Te-chato, S.
doaj  

Methods for producing and transforming cassave protoplasts

, 1996
Method for producing protoplasts of cassave or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassave of closely related species and isolating
Visser, R.G.F., Raemakers, C.J.J.M., Jacobsen, E., Bergervoet-van Deelen, J.H.M.   +3 more
core  

α‐1,3‐Glucan Is Required for Cell Wall Integrity and Full Virulence of Colletotrichum graminicola, but Not for Evasion of PAMP‐Triggered Immunity

Molecular Microbiology, EarlyView.
Abbreviated Summary The role of the major fungal cell wall polymer α‐1,3‐glucan in plant infection is poorly understood. Deletion mutants of the maize anthracnose fungus Colletotrichum graminicola lacking all three α‐1,3‐glucan synthase genes differentiated severely distorted biotrophic hyphae in planta, and virulence was strongly reduced.
Iris Gase, Lala Aliyeva‐Schnorr, Michael Melzer, Maria Ott, Julia Haufe, Matthias Menzel, Timo Engelsdorf, Christiane Rohrbach, Alan de Oliveira Silva, Lars M. Voll, Alexander S. Mickel, Maximilian Groß, Mamoru Wakayama, Holger B. Deising   +13 more
wiley   +1 more source

Application of the comet assay in studies of programmed cell death (PCD) in plants

Acta Societatis Botanicorum Poloniae, 2014
Programmed cell death (PCD) in plants is an intensively investigated process. One of the main characteristics of PCD in both animal and plant organisms is the non-random, internucleosomal fragmentation of nuclear DNA, usually analysed using total DNA gel
Maria Charzyńska, Ewa Simeonova, Adam Sikora, Agnieszka Mostowska, Joanna Leśniewska   +4 more
doaj   +1 more source