Results 51 to 60 of about 3,956,989 (242)

Optimization of protoplast preparation conditions of Pleurotus giganteus

open access: yesGuangxi Zhiwu, 2023
In order to obtain the optimal conditions for the protoplast preparation of Pleurotus giganteus, two strains, PG46 and PG79, with different temperature types, were selected as materials to study the effects of five factors (mycelial age, osmotic ...
Yongru PIAN   +5 more
doaj   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Extra thin alginate film: an efficient technique for protoplast culture

open access: yes, 2005
This paper reports an efficient protoplast culture technique, the “extra thin alginate film’’ technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various ...
Sharma, Madhu   +2 more
core   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

open access: yesPlant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar   +4 more
doaj   +1 more source

Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry

open access: yesCytometry Part A, EarlyView.
ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian   +3 more
wiley   +1 more source

Protoplast isolation and culture for banana regeneration via somatic embryogenesis

open access: yes, 2009
Introduction. This protocol describes a method for obtaining protoplasts from banana leaves, calli and cell suspensions, and their sustainable development via somatic embryogenesis from embryogenic cell suspensions.
Abdelkarim Guedira   +3 more
core   +1 more source

Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.

open access: yesBMC Plant Biology
Background Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B. oleracea var.
Katarzyna Stelmach-Wityk   +3 more
doaj   +1 more source

Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)

open access: yesOpen Life Sciences, 2017
Recent research progresses on tissue culture (e.g. fast reproduction, another culture, protoplast culture and organogenesis) and genetic transformation of kenaf were reviewed and summarized in this paper.
An Xia   +14 more
doaj   +1 more source

Salt‐induced nutritional and metabolic shifts in halophytes: implications for food security

open access: yesJournal of the Science of Food and Agriculture, EarlyView.
Abstract Plant species vary in their response to salinity: some crops show a degree of salt tolerance, while halophytes – whether wild or cultivated – are characterized by a high capacity to thrive under saline conditions. Halophytes are considered a source of valuable secondary metabolites with potential economic value, yet they might also produce ...
Giulia Atzori   +9 more
wiley   +1 more source

Barley scutellum protoplasts: isolation, culture and plant regeneration

open access: yes, 1996
Many applications of cereal protoplast culture systems are still limited by the difficulties of regeneration from suspension cells which are the usual protoplast source.
Davey, M. R., Nobre, J., Lazzeri, P. A.
core   +1 more source

Home - About - Disclaimer - Privacy