Results 21 to 30 of about 3,956,989 (242)

Plant regeneration from leaf mesophyll derived protoplasts of cassava (Manihot esculenta Crantz)

PLoS ONE, 2022
A high yield of isolated protoplast and reliable regeneration system are prerequisite for successful somatic hybridization and genome editing research.
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Ngugi, Richard Oduor, Wilton Mwema Mbinda   +5 more
doaj   +2 more sources

A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]

Plant Methods, 2023
Background Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance.
Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi, Shiv Shanker Pandey   +4 more
doaj   +1 more source

ISOLATION AND CULTURE OF DAYLILY MESOPHYLL PROTOPLASTS

HortScience, 1993
Mesophyll protoplasts were isolated from a diploid daylily ( Hemerocallis cv. `Red Magic') by enzyme digestion with a solution containing 0.5% Pectolyase Y-23, Cellulase R-10 1.0%, Driselase 0.1%, Sorbitol 0.6M and half-strength MS inorganic salts at 60 rpm for 4 h.
J T, Ling, R J, Sauve
openaire   +2 more sources

Explant-dependent receptivity to isolation and a cell-wall resynthesis in protoplast culture of recalcitrant yellow lupin [PDF]

Journal of Central European Agriculture, 2013
Cell-wall resynthesis was studied in protoplast culture of yellow lupin (Lupinus luteus L.). We optimized protoplast isolation and found that explants excised from young seedling were more suitable sources of protoplasts, in contrast to callus tissue ...
Alina WISZNIEWSKA, Anna PINDEL
doaj   +1 more source

Genetic improvement of Oenological Interesting Lucanian Grapevine Varieties through CRISPR/Cas9-Mediated Mutagenesis: Somatic embryogenesis and protoplast regeneration [PDF]

BIO Web of Conferences
Cultivated grapevine varieties, propagated vegetatively, exhibit intra-varietal genetic variability due to somatic mutations. While this variability has been exploited in the past for clonal selection, current environmental challenges and market demands ...
Giorio Giovanni, Stigliani Adriana Lucia, Zaccagnino Antonella, Nuzzo Vitale, D’Ambrosio Caterina   +4 more
doaj   +1 more source

Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review

Horticulturae, 2022
In vitro plant tissue culture and biotechnology used to assist and support the development of plant breeding when classical methods of propagation must be accelerated or it was necessary to overcome barriers inaccessible by classical approaches.
Carlos Lopez Encina, José Javier Regalado   +1 more
doaj   +1 more source

A simple culture method forBrassica hypocotyl protoplasts [PDF]

Plant Cell Reports, 1985
Hypocotyl protoplasts from oil rape, Brassica napus L. cv. Isuzu were cultured in the dark at 25°C in a modified Nitsch and Nitsch medium containing 13% sucrose, 5 g/l Ficoll, 0.5 mg/l BAP, 1 mg/l NAA and 0.5 mg/l 2-4 D. Protoplasts floated on the surface of the medium and developed into microcolonies 0.5 mm in diameter in 4-6 weeks.
P V, Chuong, K P, Pauls, W D, Beverdorf
openaire   +3 more sources

Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn

BMC Plant Biology, 2023
Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek, Reneé Pérez-Pérez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus   +4 more
doaj   +1 more source

Intermolecular Carbon–Carbon Naphthalene Coupling Involves a Radical Mechanism Driven by Cytochrome P450 in Alternaria alternata

Angewandte Chemie, EarlyView.
1,8‐DHN is an important intermediate to form complex aromatic ring systems. Phenol‐coupling reactions are important for the dimerization of two molecules. Here, we show that a fungal cytochrome P450 enzyme catalyzes specific C─C bonds. ABSTRACT Dihydroxynaphthalene (DHN) derivatives are precursors of biologically important molecules, including melanin ...
Jia Gao, Leyao Chen, Karin Steffen, Henrik Schweder, Birgit Schreckenberger, Shu‐Ming Li, Ben Auxier, Antonis Rokas, Reinhard Fischer   +8 more
wiley   +2 more sources

Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

Frontiers in Plant Science, 2021
Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.).
Xueyuan Li, Sjur Sandgrind, Oliver Moss, Rui Guan, Emelie Ivarson, Eu Sheng Wang, Selvaraju Kanagarajan, Li-Hua Zhu   +7 more
doaj   +1 more source