Results 11 to 20 of about 393,999 (153)

Plant Breeding Through Protoplast Fusion [PDF]

Jurnal Biologi Universitas Andalas, 2020
Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time.
Devi Armita
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Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

Biology, 2020
Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in ...
Hyun Hee Kang, Aung Htay Naing, Chang Kil Kim   +2 more
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Mitochondrial Genome of Callus Protoplast Has a Role in Mesophyll Protoplast Regeneration in Citrus: Evidence From Transgenic GFP Somatic Homo-Fusion

Horticultural Plant Journal, 2017
Protoplast fusion has great potential in citrus improvement. Although citrus mesophyll protoplasts usually cannot divide and regenerate, symmetric protoplast fusion of embryogenic callus protoplast + mesophyll protoplast sometimes results in the ...
Xiaodong CAI, Jing FU, Wenwu GUO
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Early protoplast culture and partial regeneration in Cannabis sativa: gene expression dynamics of proliferation and stress response [PDF]

Frontiers in Plant Science
Cannabis sativa L. is a plant of significant interest due to its high content of biologically active compounds, durable fibers, and bioeconomic potential.
Daniel Král, Josef Baltazar Šenkyřík, Vladan Ondřej   +2 more
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Protoplast transformation of recalcitrant alkaliphilic Bacillus sp. with methylated plasmid DNA and a developed hard agar regeneration medium. [PDF]

PLoS ONE, 2011
Among the diverse alkaliphilic Bacillus strains, only a little have been reported to be genetically transformed. In this study, an efficient protoplast transformation procedure was developed for recalcitrant alkaliphilic Bacillus sp. N16-5. The procedure
Chenghua Gao, Yanfen Xue, Yanhe Ma
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Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage

BMC Plant Biology
Background Methylation is a major DNA modification contributing to the epigenetic regulation of nuclear gene expression and genome stability. DNA methyltransferases (DNMT) inhibitors are widely used in epigenetic and cancer research, but their biological
Agnieszka Kiełkowska, Agnieszka Brąszewska   +1 more
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First Report on Mesophyll Protoplast Isolation and Regeneration System for the Duboisia Species

Plants, 2023
The Duboisia species, a group of plants native to Australia, have been historically valued for their pharmacological properties and have played a significant role in traditional medicine and pharmaceutical research.
Yuxin Xue, Jayeni Chathurika Amarathunga Hiti-Bandaralage, Zhangpan Hu, Zizhu Zhao, Neena Mitter   +4 more
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Comparison of the regeneration ability in leaf-derived protoplast cultures of the cultivated Apiaceae

Scientia Horticulturae
Protoplast-based technology is widely applied across many economically important plants. However, in the Apiaceae family, studies on protoplast-to-plant regeneration systems have primarily focused on carrot and a few other crop species.
Ewa Grzebelus, Aneta Malec-Pala, Katarzyna Stelmach-Wityk, Dariusz Kadluczka, Emilia Morańska, Tomasz Oleszkiewicz   +5 more
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Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Brassica oleracea var. capitata L.) Cultivars

Plants, 2023
Protoplasts are a versatile tool in plant biotechnology since they can be used for basic biological studies as well as for breeding strategies based on genome editing.
Ester Stajič
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PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

Journal of Fungi
Protoplast-based transformation is a vital tool for genetic studies in fungi, yet no protoplast method existed for P. sclerotiorum-scaumcx01 before this study. Here, we optimized protoplast isolation, regeneration, and transformation efficiency.
Israt Jahan, Qilin Yang, Zijun Guan, Yihan Wang, Ping Li, Jian Yan   +5 more
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