Results 1 to 10 of about 904,217 (264)

Electrochemical protoplast fusion in citrus

Plant Cell Reports, 2005
We report here the development of a novel protoplast fusion method for citrus somatic hybridization. This new procedure, which we have named electrochemical protoplast fusion, is based on chemically induced protoplast aggregation, using a low concentration of polyethylene glycol, and DC pulse-promoted membrane fusion.
Luis Navarro, Núria Duran-Vila
exaly   +5 more sources

An asymmetric protoplast fusion and screening method for generating celeriac cybrids [PDF]

Scientific Reports, 2021
Celeriac F1 hybrid seed production is currently complicated due to the instability of cytoplasmic male sterile lines. To develop alternative alloplasmic CMS lines, an asymmetric protoplast fusion and hybrid screening methodology was established. Celeriac
Silvia Bruznican, Tom Eeckhaut, Johan Van Huylenbroeck, Ellen De Keyser, Hervé De Clercq, Danny Geelen   +5 more
doaj   +2 more sources

Microfluidic Single-Cell Study on Arabidopsis thaliana Protoplast Fusion—New Insights on Timescales and Reversibilities [PDF]

Plants
Plant cells are omnipotent and breeding of new varieties can be achieved by protoplast fusion. Such fusions can be achieved by treatment with poly(ethylene glycol) or by applying an electric field. Microfluidic devices allow for controlled conditions and
Thorsten Seidel, Philipp Johannes Artmann, Ioannis Gkekas, Franziska Illies, Anna-Lena Baack, Martina Viefhues   +5 more
doaj   +2 more sources

Enhancement of cellulolytic enzyme production from intrageneric protoplast fusion of Aspergillus species and evaluating the hydrolysate scavenging activity [PDF]

Microbial Cell Factories
Background Lignocellulosic biomass provides a great starting point for the production of energy, chemicals, and fuels. The major component of lignocellulosic biomass is cellulose, the employment of highly effective enzymatic cocktails, which can be ...
Doaa A. Goda, Huda M. Shakam, Mai E. Metwally, Hager A. Abdelrasoul, Mohamed M. Yacout   +4 more
doaj   +2 more sources

Development of a Robust Saccharomyces cerevisiae Strain for Efficient Co-Fermentation of Mixed Sugars and Enhanced Inhibitor Tolerance through Protoplast Fusion [PDF]

Microorganisms
The economical and efficient commercial production of second-generation bioethanol requires fermentation microorganisms capable of entirely and rapidly utilizing all sugars in lignocellulosic hydrolysates.
Jianzhi Zhao, Yuping Zhao, Longhao Wu, Ning Yan, Shuo Yang, Lili Xu, Deyun He, Hongxing Li, Xiaoming Bao   +8 more
doaj   +2 more sources

Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.

Agriculture, 2021
Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed, Miao Miao, Emmanouil D. Pratsinakis, Hongliang Zhang, Wei Wang, Yuan Yuan, Meiling Lyu, Junaid Iftikhar, Ahmed F. Yousef, Panagiotis Madesis, Binghua Wu   +10 more
doaj   +2 more sources

Breeding of Early Maturity, High-Quality Auricularia heimuer Strains Using Protoplast Fusion [PDF]

Journal of Fungi
Auricularia heimuer is an important cultivated edible mushroom in China. This study used the early maturity, chrysanthemum-shaped, high yield strain “8808” and the late-maturity, single-shaped, high-quality strain “HW15” as parental strains.
Xin Yue, Shuyuan Song, Xiaojia Zhang, Yanshu Zhao, Yuxin Jiang, Shuyang Zhou, Xiancheng Zhang, Chuang Han, Xiaodong Dai   +8 more
doaj   +2 more sources

Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants [PDF]

Plants
As unicellular systems, protoplasts derived from medicinal plant cells exhibit high totipotency and hold significant value in applications such as gene function analysis, genetic improvement, and cell engineering optimization.
Zijin Fang, Yanheng Hu, Zijing Zhou, Huijie Ma, Lingxiao Zhang, Yuting Peng, Xiaori Zhan, Yiming Sun, Chenjia Shen   +8 more
doaj   +2 more sources

Multilocus identification and genetic enhancement of Trichoderma spp. for entomopathogenic activity against Spodoptera littoralis [PDF]

Microbial Cell Factories
Background The Egyptian cotton leafworm (Spodoptera littoralis) is a highly destructive, pesticide-resistant pest affecting over 80 economically important crops across the Mediterranean and African regions.
Nehal A. Atta, Abdelmegid I. Fahmi, Khalid S. Abdel-Lateif, Hesham H. Nagaty, Enas M. Abd EL-Ghany   +4 more
doaj   +2 more sources

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Frontiers in Genome Editing, 2021
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA ...
Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin   +6 more
doaj   +1 more source