Results 1 to 10 of about 1,533,500 (299)

Platelet-specific P2Y1 receptor deficient mice have suppressed pulmonary leukocyte recruitment in response to lipopolysaccharide

Respiratory Research
A role for the P2Y1 receptor (P2Y1R) in platelet-driven inflammation has been established using a pharmacological approach, limited to an acute 4 h time span.
Dingxin Pan, Alembert Lino-Alvarado, Richard T. Amison, Tolga Oralman, Reah Evans, Oliver Baker, Graham Cocks, Clive P. Page, Simon C. Pitchford   +8 more
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Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy

Genome Medicine
Background Chimeric antigen receptor T (CAR-T) cell therapy has shown remarkable success in treating hematologic malignancies. However, its efficacy against solid tumors remains limited. One of the major challenges is the lack of specific tumor antigens.
Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du   +10 more
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Genetic Alternatives for Experimental Adaptation to Colistin in Three Pseudomonas aeruginosa Lineages

Antibiotics
Pseudomonas aeruginosa is characterized by a high adaptive potential, developing resistance in response to antimicrobial pressure. We employed a spatiotemporal evolution model to disclose the pathways of adaptation to colistin, a last-resort polymyxin ...
Igor Chebotar, Tatiana Savinova, Julia Bocharova, Dmitriy Korostin, Peter Evseev, Nikolay Mayanskiy   +5 more
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scSNV-seq: high-throughput phenotyping of single nucleotide variants by coupled single-cell genotyping and transcriptomics

Genome Biology
CRISPR screens with single-cell transcriptomic readouts are a valuable tool to understand the effect of genetic perturbations including single nucleotide variants (SNVs) associated with diseases.
Sarah E. Cooper, Matthew A. Coelho, Magdalena E. Strauss, Aleksander M. Gontarczyk, Qianxin Wu, Mathew J. Garnett, John C. Marioni, Andrew R. Bassett   +7 more
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p53-regulated SESN1 and SESN2 regulate cell proliferation and cell death through control of STAT3

Cell Communication and Signaling
Sestrin1 and Sestrin2 (SESN1&2) are evolutionarily conserved, stress-responsive proteins that regulate cell growth and viability. The primary target of Sestrins is the mTORC1 protein kinase, an activator of anabolic processes and an autophagy inhibitor ...
Alexander Haidurov, Andrei O. Zheltukhin, Anastasiya V. Snezhkina, George S. Krasnov, Anna V. Kudryavtseva, Andrei V. Budanov   +5 more
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Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells

Biomedicines
T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this
Natalia Kruglova, Mikhail Shepelev
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Heterozygous deletion of 10q24.31-q24.33– a new syndrome associated with multiple congenital anomalies: case report and literature review

Neurological Research and Practice
Background Congenital anomalies and neurodevelopmental disorders are complex conditions often requiring comprehensive diagnostic approaches. Next-generation sequencing (NGS), particularly whole-exome sequencing (WES), has greatly improved the detection ...
Anastasiia A. Buianova, Yulia S. Lashkova, Tatiana V. Kulichenko, Ivan S. Kuznetsov, Artem A. Ivanov, Olga P. Parshina, Oleg N. Suchalko, Svetlana S. Vakhlyarskaya, Dmitriy O. Korostin   +8 more
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Lung Organoids from hiPSCs Can Be Efficiently Transduced by Recombinant Adeno-Associated Viral and Adenoviral Vectors

Biomedicines
Background: Organoids are a valuable model for studying hereditary diseases such as cystic fibrosis (CF). Recombinant adenoviral (rAdV) and adeno-associated viral (rAAV) vectors are promising tools for CF gene therapy and genome editing.
Lyubava Belova, Anna Demchenko, Anastasia Erofeeva, Konstantin Kochergin-Nikitsky, Olga Zubkova, Olga Popova, Tatiana Ozharovskaia, Diana Salikhova, Anna Efremova, Alexander Lavrov, Svetlana Smirnikhina   +10 more
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Transfer of mitochondrial DNA into the nuclear genome during induced DNA breaks

Nature Communications
Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery.
Jinchun Wu, Yang Liu, Liqiong Ou, Tingting Gan, Zhengrong Zhangding, Shaopeng Yuan, Xinyi Liu, Mengzhu Liu, Jiasheng Li, Jianhang Yin, Changchang Xin, Ye Tian, Jiazhi Hu   +12 more
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Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope

Nature Communications
The compact CRISPR-Cas12f system is promising for AAV-delivered gene therapy, but its application has been constrained by restrictive PAM recognition (e.g., TTTR) and suboptimal editing efficiency.
Yanan Huo, Jiale Mei, Dan Zhang, Bing Yan, Dexin Zhang, Chao Dong, Shuming Yin, Meizhen Liu, Xinyan Wang, Dan Chen, Yuting Guan, Gaojie Song, Bing Du, Yongming Wang, Zongli Zheng, Hong Liu, Dali Li, Lei Yang, Liren Wang   +18 more
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