Results 21 to 30 of about 630,749 (290)
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
Nature Communications, 2018 Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
Su Bin Moon +10 more
doaj +1 more source
Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo
Scientific Reports, 2022 Sialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in ...
Mijung Oh +14 more
doaj +1 more source
Enhancement of homology-directed repair with chromatin donor templates in cells. [PDF]
, 2020 A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template.
Cruz-Becerra, Grisel, Kadonaga, James T
core +1 more source
Mitochondrial DNA. Part B. Resources, 2020 The family Provannidae is endemic to chemosynthetic environments in the deep-sea. Here, we report the complete mitogenome of a provannid vent snail Alviniconcha boucheti from the North Fiji Basin for the first time. The length of mitogenome was 15,981 bp,
Won-Kyung Lee +3 more
doaj +1 more source
New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
Life, 2022 The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from Lachnospiraceae bacterium (LbCas12a) is widely used for biomedical research.
Mariia A. Misiurina +5 more
doaj +1 more source
Give CRISPR a chance : the GeneSprout Initiative [PDF]
, 2020 Did you know that a group of early-career researchers launched an initiative enabling open dialog on new plant breeding techniques, such as genome editing?
Boer, Damian +8 more
core +2 more sources
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]
, 2017 Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng +7 more
core +1 more source
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Advances in biotechnology: genomics and genome editing [PDF]
, 2017 Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso +4 more
core +1 more source
CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
core +1 more source