Genome editing to the rescue: sustainably feeding 10 billion global human population [PDF]
, 2016 Modern animal breeding strategies based on population genetics, molecular tools,artificial insemination,embryo transfer and related technologies have contributed to significant increases in the performance of domestic animals, and are the basis for a ...
Whitelaw, C. B. A. +11 more
core +1 more source
Assessment of the Regulatory and Institutional Framework for Agricultural Gene Editing via CRISPR-based Technologies in Latin America and the Caribbean [PDF]
, 2023 Genome editing tools have promised tremendous opportunities in agriculture for breeding crops and livestock across the food supply chain. Potentially addressing issues associated with a growing global population, sustainability concerns, and possibly ...
Inter-American Development Bank
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Scientific Reports, 2021 Transforming growth factor beta (TGF-β) is the main cytokine responsible for the induction of the epithelial-mesenchymal transition of breast cancer cells, which is a hallmark of tumor transformation to the metastatic phenotype.
Alisa M. Gorbacheva +6 more
doaj +1 more source
De Novo Transcriptome Profiling of Brain Tissue from the Annual Killifish Nothobranchius guentheri
Life, 2021 Nothobranchius is a genus of small annual killifish found in Africa. Due to the relatively short lifespan, as well as easy breeding and care, Nothobranchius fish are becoming widely used as a vertebrate model system. Studying the genome and transcriptome
Zulfiia G. Guvatova +14 more
doaj +1 more source
Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]
E3S Web of Conferences, 2020 Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu. +4 more
doaj +1 more source
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
Nature Communications, 2018 Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
Su Bin Moon +10 more
doaj +1 more source
Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo
Scientific Reports, 2022 Sialic acid (SA) is present in glycoconjugates and important in cell–cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in ...
Mijung Oh +14 more
doaj +1 more source
The Future of Genome Editing [PDF]
Cell, 2018 The power of CRISPR is undeniable, but where is the field heading? Cell's April Pawluk caught up with Jia Chen, Weizhi Ji, and Prashant Mali to discuss the successes and challenges we can expect in the coming years. Annotated excerpts from this conversation are presented below, and the full conversation is available with the article online.
openaire +2 more sources
Mitochondrial DNA. Part B. Resources, 2020 The family Provannidae is endemic to chemosynthetic environments in the deep-sea. Here, we report the complete mitogenome of a provannid vent snail Alviniconcha boucheti from the North Fiji Basin for the first time. The length of mitogenome was 15,981 bp,
Won-Kyung Lee +3 more
doaj +1 more source
New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
Life, 2022 The RNA-guided Cas12a nuclease forms a complex with a CRISPR RNA (crRNA) to cleave the double-stranded DNA target. Among others, Cas12a protein from Lachnospiraceae bacterium (LbCas12a) is widely used for biomedical research.
Mariia A. Misiurina +5 more
doaj +1 more source