Results 21 to 30 of about 1,533,500 (299)

Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling

Cellular & Molecular Biology Letters, 2023
Background The dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is found preferentially in the inner leaflet of the plasma membrane.
Eimina Dirvelyte, Daina Bujanauskiene, Evelina Jankaityte, Neringa Daugelaviciene, Ugne Kisieliute, Igor Nagula, Rima Budvytyte, Urte Neniskyte   +7 more
doaj   +1 more source

Performance comparison of Agilent new SureSelect All Exon v8 probes with v7 probes for exome sequencing

BMC Genomics, 2022
Exome sequencing is becoming a routine in health care, because it increases the chance of pinpointing the genetic cause of an individual patient's condition and thus making an accurate diagnosis.
Vera Belova, Anna Shmitko, Anna Pavlova, Robert Afasizhev, Valery Cheranev, Anastasia Tabanakova, Natalya Ponikarovskaya, Denis Rebrikov, Dmitriy Korostin   +8 more
doaj   +1 more source

PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation

Nature Communications, 2022
It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they show ...
Zhenxing Yu, Zhike Lu, Jingjing Li, Yingying Wang, Panfeng Wu, Yini Li, Yangfan Zhou, Bailun Li, Heng Zhang, Yingzheng Liu, Lijia Ma   +10 more
doaj   +1 more source

Prospects for Plant Genome Editing

, 2023
Genome editing – the focus of this book – is a set of methods that provide opportunities to precisely and efficiently improve crop traits by tailoring genes and regulatory domains.
Woźniak-Gientka, Ewa, Sweet, Jeremy, Van Laere, Katrijn, Ricroch, Agnès, Eriksson, Dennis, Miladinović, Dragana, Eriksson, Dennis,, Sweet, Jeremy,, Woźniak-Gientka, Ewa,, Laere, Katrijn Van,, Swedish University of Agricultural Sciences., Ricroch, Agnès,, Miladinović, Dragana,   +12 more
core   +1 more source

A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
doaj   +1 more source

Genome editing for nutrient use efficiency in crops [PDF]

, 2022
Plant breeders have concentrated on increasing the yields, and in turn, the production in the last century to meet the increasing food demand, while ignoring the nutrition values of staple food crops.
Yavuz, Caner, Yağiz, Ayten Kübra, Naeem, Muhammad, Dangol, Sarbesh Das, Aksoy, Emre, Caner Yavuz, Emre Aksoy, Ayten Kübra Yağız, Muhammad Naeem, Sarbesh Das Dangol   +9 more
core   +1 more source

Transgene-Free Genome Editing in Plants

, 2022
Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known specificity.
Sprink, Thorben, Hartung, Frank, Metje-Sprink, Janina   +2 more
core   +1 more source

Eye on genome editing

Journal of Experimental Medicine, 2023
CRISPR/Cas9 genome editing techniques have the potential to treat previously untreatable inherited genetic disorders of vision by correcting mutations that cause these afflictions. Using a prime editor, Qin et al. (2023. J. Exp. Med.https://doi.org/10.1084/jem.20220776) restored visual functions in a mouse model (rd10) of retinitis pigmentosa.
Samuel W. Du, Krzysztof Palczewski
openaire   +4 more sources

Peroxiredoxins—The Underrated Actors during Virus-Induced Oxidative Stress

Antioxidants, 2021
Enhanced production of reactive oxygen species (ROS) triggered by various stimuli, including viral infections, has attributed much attention in the past years. It has been shown that different viruses that cause acute or chronic diseases induce oxidative
Inna L. Karpenko, Vladimir T. Valuev-Elliston, Olga N. Ivanova, Olga A. Smirnova, Alexander V. Ivanov   +4 more
doaj   +1 more source

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Nature Communications, 2018
Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
Su Bin Moon, Jeong Mi Lee, Jeong Gu Kang, Nan-Ee Lee, Dae-In Ha, Do Yon Kim, Sun Hee Kim, Kwangsun Yoo, Daesik Kim, Jeong-Heon Ko, Yong-Sam Kim   +10 more
doaj   +1 more source