Results 21 to 30 of about 54,280 (357)

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

Horticultural Plant Journal, 2023
Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility.
Wenhui Yang, Jiaqin Ren, Wanrong Liu, Dan Liu, Kaidong Xie, Fei Zhang, Pengwei Wang, Wenwu Guo, Xiaomeng Wu   +8 more
doaj   +1 more source

Tomato resistance to Alternaria stem canker: localization in host genotypes and functional expression compared to non-host resistance [PDF]

, 1989
The Alternaria stem canker resistance locus (Asc-locus), involved in resistance to the fungal pathogen Alternaria alternata f. sp. lycopersici and in insensitivity to host-specific toxins (AAL-toxins) produced by the pathogen, was genetically mapped on ...
Griend, E.G. van de,, Hille, J.,, Nijkamp, H.J.J.,, Tiersma, J.B.,, Witsenboer, H.M.A.,   +4 more
core   +2 more sources

Penicillin-binding proteins of protoplast and sporoplast membranes of Streptomyces griseus strains [PDF]

, 1988
Membrane-bound penicillin-binding proteins (PBPs) of two Streptomyces griseus strains that sporulate well in liquid and solid medium have been investigated during the course of their life-cycle.
CE Buchanan, G Szabó, H Nakazawa, H Ogawara, J Barabás, J Dusart, J Dusart, KE Kendrick, LM Quiros, M Leyh-Bouille, OH Lowry, S Horikawa, SL Neyman, UK Laemmli, WM Bonner   +14 more
core   +1 more source

Pengaruh penambahan enzim dan waktu inkubasi terhadap jumlah protoplas mesofil daun anggrek Dendrobium Sp

Journal of Biological Researches, 1995
This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast qualtity leaf mesophyll Dendrobium sp.
Edi Setiti Wida Utami
doaj   +1 more source

Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants [PDF]

, 2003
Ethylene-induced ripening in tomato (Lycopersicon esculentum) resulted in the accumulation of a transcript designated LeEF-Tsmt that encodes a protein with significant homology to bacterial Ts translational elongation factor (EF-Ts).
Benichou, Mohamed, Bouzayen, Mondher, Chaves, Ana, Delalande, Corinne, Jauneau, Alain, Latché, Alain, Li, Zhengguo, Pech, Jean-Claude, Spremulli, Linda L., Tournier, Barthélémy, Zegzouti, Hicham   +10 more
core   +2 more sources

Reversion of Streptococcal Protoplasts [PDF]

Journal of Bacteriology, 1972
The reversion of protoplasts of Streptococcus faecium was examined by light and scanning electron microscopy. Regularly shaped streptococci emerged from the narrow region of large, oval protoplasts.
D J, Bibel, J W, Lawson
openaire   +2 more sources

The Determination of Protoplast Culture’s Efficiency with Chickpea (Cicer arietinum L.) and Grass Pea (Lathyrus sp.) Species Grown in Vitro Conditions

ISPEC Journal of Agricultural Sciences
Anthracnose (Ascochyta rabiei Labr.) disease, which causes significant economic damage in chickpea (Cicer arietinum L.) cultivation, is widespread in Türkiye, particularly in Central Anatolia.
Ramazan KOYUN, Ercan CEYHAN
doaj   +1 more source

Different 3' end regions strongly influence the level of gene expression in plant cells [PDF]

, 1989
We have investigated the functional role of a 3' end region on the expression of a reporter gene in plant cells. In stably transformed plants, expression of the reporter gene without a plant gene 3' end is variable and depends on the fortuitous presence ...
Ingelbrecht, Ivan, Herman, Lieve MF, Dekeyser, Rudy, Van Montagu, Marc, Depicker, Anna   +4 more
core   +1 more source

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes [PDF]

, 2008
Gene silencing in fungi is often induced by dsRNA hairpin forming constructs the preparation of which can require multiple cloning steps. To simplify gene silencing in the filamentous fungi we have evaluated a high throughput cloning method for target ...
Bailey, Andy M., Challen, Michael P., Costa, Ana M.S.B., Foster, Gary D., Mills, P. R.   +4 more
core   +1 more source