Results 21 to 30 of about 56,071 (291)

Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation

Plants, 2021
Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant ...
T. Pasternak, I. Paponov, S. Kondratenko
semanticscholar   +1 more source

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines

BMC Research Notes, 2023
Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela, Danna Susunaga-Gómez, Catalina Restrepo-Osorio, Carsten Harms, Diego Villanueva-Mejía   +4 more
doaj   +1 more source

Research Progress of Algae Protoplast Fusion Technique [PDF]

, 2022
This paper discussed the technique of algal protoplast fusion, including the method of protoplast-induced fusion, the screening and identification of hybrid cells and so on.
XU, Xiaoying, LI, Lei, CAO, Yanan, LU, Xiaoguang, LIU, Peng, SHI, Wenkai   +5 more
core   +1 more source

Protoplast Isolation and Transformation in Oil

, 2022
187202The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol (PEG)-mediated transfection and DNA microinjection, highlighting also the critical steps associated with the ...
Noll, Gundula, Ghulam Kadir Ahmad Parveez, Dirk Pruefer, Mat Yunus Abdul Masani, Gundula Noll, M. D. Piji Mohd Al Akmarul Fizree, Parveez, Ghulam Kadir Ahmad, Ravigadevi Sambanthamurthi, Fizree, M. D. Piji Mohd Al Akmarul, Sambanthamurthi, Ravigadevi, Masani, Mat Yunus Abdul, Prüfer, Dirk   +11 more
core   +1 more source

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

BMC Plant Biology, 2020
To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, the establishment of a PEG-mediated banana protoplast transformation system makes it possible to build an efficient DNA-free method for a site ...
Shaoping Wu, Haocheng Zhu, Jin-Xing Liu, Qiaosong Yang, Xiu-Hong Shao, Fangcheng Bi, Chun-hua Hu, H. Huo, Kun-Ling Chen, Ganjun Yi   +9 more
semanticscholar   +1 more source

Phytosulfokine Peptide Library: Chemical Synthesis and Biological Evaluation on Protoplast Regeneration [PDF]

, 2023
Phytosulfokine (PSK) is a highly conserved plant peptide hormone (PPH) containing two sulfated tyrosine residues. PSK acts as a growth factor in Arabidopsis thaliana plant species and significantly improves the efficiency of cellular proliferation in ...
Dieuwertje, Streefkerk, Richard, Immink, Jasper, van de Sande, Bauke, Albada, Martijn, Fiers   +4 more
core   +2 more sources

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

Frontiers in Genome Editing, 2021
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA ...
Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin   +6 more
doaj   +1 more source

Development of an efficient leaf protoplast isolation and transient expression system for Artemisia japonica

Biologia Plantarum, 2023
Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng, X. Fu, B. Peng, Q. Miao, S. Zeng, K. Tang, Q. Pan   +6 more
doaj   +1 more source

Effect of Different Cellulase and Pectinase Enzyme Treatments on Protoplast Isolation and Viability in Lilium ledebeourii Bioss.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012
For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant withdesired traits. For this purpose, protoplast preparation is a first and important step.
Esmaeil CHAMANI, Seyyed Karim TAHAMI, Nasser ZARE, Rasool Asghari-ZAKARIA, Mehdi MOHEBODINI, Daryl JOYCE   +5 more
doaj   +1 more source

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids

International Journal of Molecular Sciences, 2020
Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for Cymbidium orchids.
R. Ren, Jie Gao, Chuqiao Lu, Yonglu Wei, Jianpeng Jin, Sek-Man Wong, Gen-Fa Zhu, Fengxi Yang   +7 more
semanticscholar   +1 more source