Results 31 to 40 of about 26,488 (218)

Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. [PDF]

open access: yesPLoS ONE, 2012
BackgroundPopulus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.Methodology/principal findingsWe optimized a Populus leaf mesophyll
Jianjun Guo   +6 more
doaj   +1 more source

TED1 Promotes the Assembly of SCFD3‐D14‐D53 to Reinforce Strigolactone Signaling in Rice

open access: yesAdvanced Science, EarlyView.
This study identifies TED1 as a positive regulator of strigolactones (SL) signaling in rice. The ted1 mutants display dwarfism and increased tillering with significantly reduced SLs sensitivity. Through its longer C‐terminal region, TED1 interacts with D53 and D14 to promote the assembly of SCFD3‐D14‐D53, thereby facilitating the efficient ...
Sheng Luo   +22 more
wiley   +1 more source

Marchantia Protoplast Isolation v1

open access: yes, 2016
Marchantia protoplast preparation (adapted from Bopp and Vicktor 1988)
Christian Boehm
core   +1 more source

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato (Lycopersicon esculentum Mill.) protoplast yield

open access: yesActa Agrobotanica, 2013
The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was
Elżbieta Kuźniak   +3 more
doaj   +1 more source

The BTB/POZ‐MATH Protein ZmBPM1 Interacts With Autophagy‐related Protein ZmATG6 and Modulates the NLR Protein Rp1‐D21‐Mediated Defense Response in Maize

open access: yesAdvanced Science, EarlyView.
The maize BTB/POZ‐MATH (BPM) protein ZmBPM1 interacts with autophagy‐related protein ZmATG6 to relocate the NLR protein Rp1‐D21 from the nucleo‐cytoplasmic compartment into autophagosome‐like puncta, leading to autophagy‐mediated turnover and suppressing the hypersensitive response.
Chang‐Xiao Tang   +8 more
wiley   +1 more source

OsCPK12‐OsATPD1 Module Regulates Photosynthetic Acclimation to Light Stress in Rice

open access: yesAdvanced Science, EarlyView.
OsCPK12 interacts and phosphorylates OsAtpD1 at Ser8 in its transit peptide. This phosphorylation promote its chloroplast translocation, thereby maintaining ATP synthase activity and proton gradient (ΔpH) homeostasis and regulating photosynthetic acclimation, photoprotection, and repair under light stress.
Beifang Wang   +17 more
wiley   +1 more source

The MoSR‐MoDde1 Regulatory Axis: A Novel Determinant of DMI Sensitivity and a Target for Sustainable Rice Blast Control

open access: yesAdvanced Science, EarlyView.
The transcription factor MoSR governs DMI fungicide sensitivity in Magnaporthe oryzae through dual regulation of ergosterol biosynthesis and the detoxification enzyme MoDde1. Targeting MoDde1 via a small‐molecule inhibitor or host‐induced gene silencing enhances DMI efficacy against rice blast, offering a sustainable disease management strategy ...
Fan‐Zhu Meng   +10 more
wiley   +1 more source

Enhancing Protoplast Isolation and Early Cell Division from Cannabis sativa Callus Cultures via Phenylpropanoid Inhibition

open access: yesPlants
De novo regeneration of Cannabis sativa L. (cannabis) using tissue culture techniques remains unreliable and infrequent. Conventional methods for the regeneration and transformation of cannabis have not achieved the reliability and replicability that ...
Adrian S. Monthony   +1 more
doaj   +1 more source

Protoplast Regeneration and Its Use in New Plant Breeding Technologies

open access: yesFrontiers in Genome Editing, 2021
The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need to feed a growing global population.
Kelsey M. Reed, Bastiaan O. R. Bargmann
doaj   +1 more source

Dual Repression by IPA1 Fine‐Tunes OsbZIP79‐Mediated Salt Tolerance in Rice

open access: yesAdvanced Science, EarlyView.
Dual repression by IPA1 fine‐tunes OsbZIP79‐mediated salt tolerance in rice: direct transcriptional inhibition under normal conditions and salt‐induced degradation under stress. This dual mechanism activates OsbZIP79 to regulate Na+/K+ homeostasis and redox balance via downstream genes, enabling optimal salt stress response.
Hui Wang   +12 more
wiley   +1 more source

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