Results 31 to 40 of about 56,071 (291)

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

Frontiers in Plant Science, 2016
The combined availability of whole genome sequences and genome editing tools is set to revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic changes with unprecedented control and accuracy, both to explore ...
M. Malnoy, R. Viola, M. Jung, O. Koo, Seokjoong Kim, Jin-Soo Kim, R. Velasco, Chidananda Nagamangala Kanchiswamy   +7 more
semanticscholar   +1 more source

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

Plant Biotechnology Journal, 2018
Summary Plant protoplasts are useful for assessing the efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) mutagenesis.
Choun-Sea Lin, Chen-Tran Hsu, Ling-Hung Yang, Lan-Ying Lee, Jin-Yuan Fu, Qiao-Wei Cheng, Fu-Hui Wu, C.-W.-Hsiao Han, Yesheng Zhang, Ru Zhang, Wan-Jung Chang, Chen-Ting Yu, Wen Wang, L. Liao, S. Gelvin, Ming-Che Shih   +15 more
semanticscholar   +1 more source

Optimization of protoplast isolation, transformation and its application in sugarcane (Saccharum spontaneum L)

, 2020
Sugarcane is a prominent source of sugar and ethanol production. Genetic analysis for trait improvement of sugarcane is greatly hindered by its complex genome, long breeding cycle, and recalcitrance to genetic transformation.
Qiongli Wang, Guangrun Yu, Zhiyong Chen, Jin-Lei Han, Yu-Feng Hu, Kai Wang   +5 more
semanticscholar   +1 more source

Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.

Plant Cell Tissue and Organ Culture, 2020
In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis in callus cultures with subsequent plant regeneration, and (3) isolation and ...
M. Klimek-Chodacka, Dariusz Kadluczka, A. Lukasiewicz, Aneta Malec-Pala, R. Barański, E. Grzebelus   +5 more
semanticscholar   +1 more source

Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase

Makara Journal of Science, 2014
The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production.
Wijanarka Wijanarka, Endang Sutariningsih Soetarto, Kumala Dewi, Ari Indrianto   +3 more
doaj   +1 more source

Somaclonal Variation in Chrysanthemum × morifolium Protoplast Regenerants

Frontiers in Plant Science, 2020
Chrysanthemum × morifolium protoplasts were isolated and regenerated to assess possible protoclonal variation in the regenerants. After a preliminary screening of the potential of different regeneration systems for protoplast regeneration, we produced a ...
T. Eeckhaut, Wouter Van Houtven, Silvia Bruznican, L. Leus, J. Van Huylenbroeck   +4 more
semanticscholar   +1 more source

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

Horticultural Plant Journal, 2023
Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility.
Wenhui Yang, Jiaqin Ren, Wanrong Liu, Dan Liu, Kaidong Xie, Fei Zhang, Pengwei Wang, Wenwu Guo, Xiaomeng Wu   +8 more
doaj   +1 more source

Pengaruh penambahan enzim dan waktu inkubasi terhadap jumlah protoplas mesofil daun anggrek Dendrobium Sp

Journal of Biological Researches, 1995
This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast qualtity leaf mesophyll Dendrobium sp.
Edi Setiti Wida Utami
doaj   +1 more source

Penicillin-binding proteins of protoplast and sporoplast membranes of Streptomyces griseus strains [PDF]

, 1988
Membrane-bound penicillin-binding proteins (PBPs) of two Streptomyces griseus strains that sporulate well in liquid and solid medium have been investigated during the course of their life-cycle.
Szabó, István,, Harder, Wim,, Harder, Willem, Veenhuis, Marten,, Barabás, György,, Barabás, Judit,, Veenhuis, Marten, Barabás, Judit, Barabás, György, Szabó, István   +9 more
core   +1 more source

Protoplast suspension culture.

, 2022
A. Freshly isolated protoplast. B. Dividing protoplast three days after culture initiation. C. Microcalli developing two weeks after culture initiation. D. Minicalli 4 weeks after culture initiation. E. Minicalli developing on solid medium.
Asunta Mukami (14011413), Mathew Ngugi (10494139), Richard Oduor (7408637), Bicko Steve Juma (14011410), Wilton Mwema Mbinda (14204647), Cecilia Mweu (10494133)   +5 more
core   +1 more source