Results 31 to 40 of about 698,061 (253)
Journal of Biological Researches, 1995 This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast qualtity leaf mesophyll Dendrobium sp.
Edi Setiti Wida Utami
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ISOLATION AND CULTURE OF DAYLILY MESOPHYLL PROTOPLASTS
HortScience, 1993 Mesophyll protoplasts were isolated from a diploid daylily ( Hemerocallis cv. `Red Magic') by enzyme digestion with a solution containing 0.5% Pectolyase Y-23, Cellulase R-10 1.0%, Driselase 0.1%, Sorbitol 0.6M and half-strength MS inorganic salts at 60 rpm for 4 h.
J T, Ling, R J, Sauve
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Comparison of protoplast isolation methods.
, 2023 Cowpea (Vigna unguiculata) is a legume staple widely grown across Sub-Saharan Africa and other tropical and sub-tropical regions. Considering projected climate change and global population increases, cowpea’s adaptation to hot climates, resistance to ...
Michael J. Thomson (8477751) +4 more
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Plant protoplast isolation - a practical approach [PDF]
Biochemical Education, 2000 A method for the isolation of plant protoplasts is presented that uses inexpensive sources of enzymes. It is suitable for student use in Biotechnology.
V. Prasad, G.R. Naik
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Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb
Journal of Applied Sciences and Environmental Management, 2013 Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
I Mukhtar, R Bajwa, G Nasim
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Protoplast Isolation - Incubation Buffer v1
, 2016 Instructions to make 50 mL of incubation buffer for protoplast isolation. Adapted from Yoo et al. 2007http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.html
Steven Burgess
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Scientia Agricola, 1995 A hibridação somática via fusão de protoplastos vem sendo utilizada no melhoramento de porta-enxertos de citros em diversos países. Nos Estados Unidos, vários estudos demonstram a eficiência de procedimentos no isolamento e cultivo de protoplastos dessa ...
R.P. de Oliveira +2 more
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A Method for Isolation of Protoplasts from Dermatophytes [PDF]
Microbiology, 1984 A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO4 has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell wall material on these protoplasts.
Srikantha, T, Rao, G Ramananda
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Isolation and transformation of rice aleurone protoplasts [PDF]
Plant Cell Reports, 1994 Protoplasts isolated from the aleurone have been used extensively in molecular studies focusing on hormone-mediated regulation of gene expression in barley seed. To extend the use of aleurone protoplasts to other species, we have determined the conditions necessary for the isolation of protoplasts from rice aleurone layers of germinated seed.
S, Sadasivam, D R, Gallie
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Plant cell protoplast isolation v1
, 2016 A protocol for isolation of protoplast from plant cells adapted from Yoo et al. 2007 (http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.htm). Procedure was optimized for use on Arabidopsis thaliana tissue.
Steven Burgess
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