Results 51 to 60 of about 21,306 (282)

Teknik Isolasi Dan Kultur Protoplas Tanaman Padi [PDF]

, 2007
Protoplastfusion or somatic hybridization technology is an alternativetechnology for production hybrids of plants that are difficultto be produced by conventional methods due to their sexualincompatibility.
Lestari, E. G. (Endang), Roostika, I. (Ika), Suharlini, T. (Tintin), Sukmadjaja, D. (Deden), Sunarlim, N. (Novianti)   +4 more
core  

Isolation and transformation of rice aleurone protoplasts [PDF]

Plant Cell Reports, 1994
Protoplasts isolated from the aleurone have been used extensively in molecular studies focusing on hormone-mediated regulation of gene expression in barley seed. To extend the use of aleurone protoplasts to other species, we have determined the conditions necessary for the isolation of protoplasts from rice aleurone layers of germinated seed.
S, Sadasivam, D R, Gallie
openaire   +3 more sources

Introgressed Variation in TaMYB7‐A1 Drives Graded Dormancy and Climate‐Adaptive Pre‐Harvest Sprouting Resistance in Wheat

Advanced Science, EarlyView.
TaMYB7‐A1 directly activates TaABI5 to enhance ABA signaling and regulate ABA‐GA homeostasis, enforcing seed dormancy. Its superior allele, derived from wild einkorn introgression, harbors a MITE insertion that elevates expression and two amino acid substitutions that enhance transcriptional activity, collectively generating graded PHS resistance for ...
Hao Wang, Dongzhi Wang, Yafei Guo, Qi Zheng, Min Zhang, James Simmonds, Jian Hou, Xuemei Liu, Xuelei Lin, Xiaomin Bie, Xiansheng Zhang, Xiaohui Li, Yuan Chen, Xueyong Zhang, Cristobal Uauy, Fei Lu, Chengcai Chu, Zhixi Tian, Jun Xiao   +18 more
wiley   +1 more source

Optimization of isolation and transfection conditions of maize endosperm protoplasts

Plant Methods, 2020
Background Endosperm-trait related genes are associated with grain yield or quality in maize. There are vast numbers of these genes whose functions and regulations are still unknown. The biolistic system, which is often used for transient gene expression,
Yufeng Hu, Dalin Song, Lei Gao, Babatope Samuel Ajayo, Yongbin Wang, Huanhuan Huang, Junjie Zhang, Hanmei Liu, Yinghong Liu, Guowu Yu, Yongjian Liu, Yangping Li, Yubi Huang   +12 more
doaj   +1 more source

Development of an efficient transformation system for Dothistromin pini : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University [PDF]

, 1996
A transformation system has been developed for the plant pathogenic fungus Dothistroma pini using a positive selection system based on the Escherichia coli hygromycin B phosphotransferase gene (hph).
Bidlake, Anita Therese
core  

Protoplast Isolation and Regeneration in Streptomyces clavuligerus [PDF]

Microbiology, 1989
The regeneration of streptomycete protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. Reports of studies on the regeneration of protoplasts from Streptomyces clavuligerus are limited and for this reason the experiments described in this paper were carried out.
G T, Illing, I D, Normansell, J F, Peberdy   +2 more
openaire   +2 more sources

OsGSK2‐OsTCP19 Module Integrates Nitrogen and Brassinosteroid Signaling to Regulate Nitrogen Utilization and Root Growth in Rice

Advanced Science, EarlyView.
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu, Weiwei Li, Xiaohui Ma, Xiaohan Wang, Yarong Shi, Xiahe Huang, Xinran Li, Yingchun Wang, Hongning Tong, Bin Hu, Wenbin Zhou, Chengcai Chu   +11 more
wiley   +1 more source

Utilization of Channels Digestion Golden Snail (Pomacea Canaliculata) as Lytic Enzyme and Application on Yeast Pichia Manshurica DUCC-Y15 [PDF]

, 2015
Mollusks were one of invertebrate animals that have not been studied and used in in the field of enzyme. One type of mollusk that has not been exploited significantly is golden snail (Pomacea canaliculata), especially in the digestive ...
Hidayat, Jafron W., Parman, Sarjana, Wijanarka, W.   +2 more
core  

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes [PDF]

, 2008
Gene silencing in fungi is often induced by dsRNA hairpin forming constructs the preparation of which can require multiple cloning steps. To simplify gene silencing in the filamentous fungi we have evaluated a high throughput cloning method for target ...
Bailey, Andy M., Challen, Michael P., Costa, Ana M.S.B., Foster, Gary D., Mills, P. R.   +4 more
core   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source