Results 61 to 70 of about 698,061 (253)

Viscosity of concentrated mRNA solutions: Implications for downstream processing

Biotechnology Progress, EarlyView.
Abstract Messenger RNA (mRNA) therapeutics can be used to protect against infectious disease and for the treatment of cancers and genetic disorders. In order to obtain the desired therapeutic dosage, it is necessary to concentrate the mRNA to levels where the viscosity of the mRNA solutions can become significant.
Amin Javidanbardan, Fatemeh Najafi, Ziqiao Wang, Andrew L. Zydney   +3 more
wiley   +1 more source

Oritavancin Binds to Isolated Protoplast Membranes but not Intact Protoplasts of Staphylococcus aureus [PDF]

Journal of Molecular Biology, 2009
Solid-state NMR has been used to examine the binding of N'-4-[(4-fluorophenyl)benzyl)]chloroeremomycin, a fluorinated analogue of oritavancin, to isolated protoplast membranes and whole-cell sucrose-stabilized protoplasts of Staphylococcus aureus, grown in media containing [1(13)C]glycine and L-[epsilon-(15)N]lysine.
Sung Joon, Kim, Manmilan, Singh, Jacob, Schaefer   +2 more
openaire   +2 more sources

Independent leaf and root responses to autumn freezing in perennial herbs: Insights from cutting and transplant manipulations

Grassland Research, EarlyView.
In this study, we compared two sampling approaches to assess autumn freezing resistance. Our goal was to determine whether the commonly employed cutting method, which disrupts root‐tissue connectivity, provides a valid measure of true freezing resistance.
Zhengjie Yan, Cheng Chen, Xin Jing, Shuai Ren, Junpeng Rui, Tao Wang, Jin‐Sheng He   +6 more
wiley   +1 more source

Efficient Mesophyll Protoplast Isolation and Development of a Transient Expression System for Castor-Oil Plant (Ricinus communis L.) [PDF]

, 2019
Introduction: We investigated the main factors affecting the efficacy of protoplast isolation, including leaf-obtaining period, cutting shapes of leaf material, enzyme concentration, enzymolysis time, and centrifugal speed.
Liu, Ying, Jianping Chen, Ying Liu, Jianian Tang, Yingbin Xue, Chen, Miao, Xue, Yingbin, Tang, Jianian, Chen, Jianping, Miao Chen   +9 more
core   +1 more source

Early protoplast culture and partial regeneration in Cannabis sativa: gene expression dynamics of proliferation and stress response

Frontiers in Plant Science
Cannabis sativa L. is a plant of significant interest due to its high content of biologically active compounds, durable fibers, and bioeconomic potential.
Daniel Král, Josef Baltazar Šenkyřík, Vladan Ondřej   +2 more
doaj   +1 more source

Subcellular Localization of Iron in Rhizophora mangle Leaves Revealed by Integrated Perls Reaction, TEM, STEM‐HAADF, and EDS Analyses

Microscopy Research and Technique, EarlyView.
Perls–TEM–STEM‐HAADF–EDS enables high‐resolution localization and elemental characterization of Fe‐containing deposits in Rhizophora mangle leaf cells. ABSTRACT Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in
Silvia Rodrigues Machado, Hiulana Pereira Arrivabene, Camilla Rozindo Dias Milanez, Margarida Juri Saeki, Yve Canaveze   +4 more
wiley   +1 more source

Marine yeast isolation and industrial application [PDF]

, 2014
Over the last century, terrestrial yeasts have been widely used in various industries, such as baking, brewing, wine, bioethanol and pharmaceutical protein production. However, only little attention has been given to marine yeasts. Recent research showed
Tucker, Gregory A., Daw, Zakaria Yehia, Tucker, Gregory A, Zaky, Abdelrahman Saleh, Du, Chenyu   +4 more
core   +1 more source

Optimal Parameters for the Isolation of Carrot Mesophyll Protoplasts In Vitro

Овощи России
The isolation of protoplasts from diverse plant species is a widely employed technique. The purpose of this work is to develop an efficient system for isolating and purifying mesophyll protoplasts from Daucus carota.
N. Aljaramany, S. G. Monakhos
doaj   +1 more source

Deciphering plant–pathogen interaction with single‐cell omics

New Plant Protection, EarlyView.
Recent advances in single‐cell omics have enabled the decoding of gene regulatory networks at the cellular resolution during pathogen challenge. This review summarizes the technological developments in plant single‐cell omics and highlights their unique advantages for dissecting nearly all stages of plant–pathogen interactions, including initial ...
Lu Gan, Wenwen Liu, Syed S. Zaidi, Huaibing Jin   +3 more
wiley   +1 more source

The optimization mesophyll protoplast isolation for Phalaenopsis amboinensis [PDF]

, 2019
Indonesia has 25 species of month orchids and 10 of them are endemic to Indonesia. Plant Conservation Center (LIPI) conserved 15 species of orchids which means that it has been conserving as much as 23.44% of the orchids in the world (Rahayu, 2015 ...
Purnobasuki, Hery, Wida Utami, Edy Setiti, Machmudi, Machmudi   +2 more
core