Results 71 to 80 of about 734,035 (251)

Subcellular Localization of Iron in Rhizophora mangle Leaves Revealed by Integrated Perls Reaction, TEM, STEM‐HAADF, and EDS Analyses

Microscopy Research and Technique, EarlyView.
Perls–TEM–STEM‐HAADF–EDS enables high‐resolution localization and elemental characterization of Fe‐containing deposits in Rhizophora mangle leaf cells. ABSTRACT Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in
Silvia Rodrigues Machado, Hiulana Pereira Arrivabene, Camilla Rozindo Dias Milanez, Margarida Juri Saeki, Yve Canaveze   +4 more
wiley   +1 more source

Optimisation of protoplast isolation protocols using in vitro leaves of Dendrobium crumenatum (pigeon orchid)

, 2010
Protoplast isolation from in vitro leaves of Dendrobium crumenatum was carried out. Factors affecting protoplast isolation, sorbitol concentration, enzyme combinations and concentration and incubation time for isolation were studied.
Lee, Pui Sinn, Ling, Anna Pick Kiong, Tee, Chong Siang, Mahmood, Maziah   +3 more
core   +1 more source

Comparison of protoplast isolation methods.

, 2023
Cowpea (Vigna unguiculata) is a legume staple widely grown across Sub-Saharan Africa and other tropical and sub-tropical regions. Considering projected climate change and global population increases, cowpea’s adaptation to hot climates, resistance to ...
Michael J. Thomson (8477751), Endang M. Septiningsih (6003947), Aya Bridgeland (15188045), Sudip Biswas (8506077), Nikolaos Tsakirpaloglou (15188048)   +4 more
core   +1 more source

Natural variation in the promoter of OsPDX1.2 enhances vitamin B6 accumulation and cold tolerance in rice

Journal of Integrative Plant Biology, EarlyView.
Allelic variation in the promoter of the pyridoxal phosphate synthase OsPDX1.2 caused variation in the ACE‐box cis‐elements, resulting in differing OsPDX1.2 expression due to differences in binding affinity between the OsbZIP18 transcriptipn factor and the ACE‐box, contributing to variation in vitamin B6 levels and cold tolerance among rice varieties ...
Bi Wang, Qiu Yi Wang, Wen Jie Shi, Rong Hui Li, Xu Dong Zhang, Li Ping Wang, Shi Jia Xia, Kai Xi Chen, Jun Jie Zhou, Yang Yang Sun, Jing He, Alisdair R. Fernie, Ying Chun Han, Jun Zhou Li, Yu Heng Shi, Xian Qing Liu, Yuan Yuan Zhang, Jie Luo, Cheng Jin   +18 more
wiley   +1 more source

Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb [PDF]

, 2013
Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
Nasim, G, Nasim, Gazala, Mukhtar, I, Bajwa, Rukhsana, Bajwa, R, Mukhtar, Irum   +5 more
core   +3 more sources

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.

Revista Brasileira de Fruticultura
To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts.
Sherif Fathy Eid El-Sayed El-Gioushy, Abdul Kareem, Mohamed Hemdan Mohamed Baiea   +2 more
doaj   +1 more source

An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP-84. [PDF]

Microbiologyopen
The study presents an optimized, reproducible protoplast transfection method for Geobacillus stearothermophilus. The method enables efficient uptake of native and fully synthetic TP‐84 bacteriophage genomes, their “rebooting” in vivo and production of infectious phage particles.
Sobolewski I, Adamowicz K, Chodorski J, Skowron PM, Zylicz-Stachula A.   +4 more
europepmc   +2 more sources

CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans

Journal of Integrative Plant Biology, EarlyView.
The small cyclic peptide CP32 effectively inhibits Phytophthora infestans, the causal agent of potato and tomato late blight. Combined biochemical and phenotypic analyses demonstrate that CP32 disrupts cell wall biosynthesis, a key process in pathogen infection, resulting in a marked reduction of disease symptoms in plants.
Demetrio Marcianò, Stefano Rosa, Elena Marone Fassolo, Chiara Bertaso, Andrea Tagliani, Nina Gauri Capra, Federico Ballabio, Chiara Mizzotti, Giulia Rizzi, Sadia Fida Ullah, Lisa Kappel, Lucia Feni, Luca Tadini, Louise Jane Gourlay, Rosaria Russo, Vincent Bulone, Sabrina Pricl, Sandrine Ongeri, Sara Pellegrino, Carlo Camilloni, Silvia Laura Toffolatti, Vaibhav Srivastava, Paolo Pesaresi, Simona Masiero   +23 more
wiley   +1 more source

Highly efficient protoplast isolation, fusion and PEG-mediated transient transformation system of pear

Scientia Horticulturae
Isolation of protoplasts from tissues of woody plants has always been a major technical bottleneck, protoplast based approaches enable gene transfer but are poorly developed in pear (Pyrus). Here, we established an efficient protoplast isolation, fusion,
Rui Zhao, Shahrokh Khanizadeh, Katsuhiro Shiratake, Shutian Tao   +3 more
doaj   +1 more source

Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1

Plant Methods, 2017
Background Magnolia is a woody ornamental plant, which is widely used in urban landscaping. However, its lengthy juvenile period and recalcitrance to regeneration impedes functional characterization of its genes.
Yamei Shen, Dong Meng, Kim McGrouther, Junhong Zhang, Lailiang Cheng   +4 more
doaj   +1 more source