Results 71 to 80 of about 21,306 (282)

Synthetic Promoter Screening Using Poplar Mesophyll Protoplast Transformation

Bio-Protocol, 2023
Plant protoplasts are useful to study both transcriptional regulation and protein subcellular localization in rapid screens. Protoplast transformation can be used in automated platforms for design-build-test cycles of plant promoters, including synthetic
Yongil Yang, Yuanhua Shao, Timothy Chaffin, Amir Ahkami, Eduardo Blumwald, C. Neal Stewart Jr.   +5 more
doaj   +1 more source

Oritavancin Binds to Isolated Protoplast Membranes but not Intact Protoplasts of Staphylococcus aureus [PDF]

Journal of Molecular Biology, 2009
Solid-state NMR has been used to examine the binding of N'-4-[(4-fluorophenyl)benzyl)]chloroeremomycin, a fluorinated analogue of oritavancin, to isolated protoplast membranes and whole-cell sucrose-stabilized protoplasts of Staphylococcus aureus, grown in media containing [1(13)C]glycine and L-[epsilon-(15)N]lysine.
Sung Joon, Kim, Manmilan, Singh, Jacob, Schaefer   +2 more
openaire   +2 more sources

Construction of an O2‐responsive engineering bioreactor for enhanced indole‐3‐propionic acid production and deoxynivalenol detoxification

Responsive Materials, EarlyView.
An oxygen‐responsive living bioreactor was engineered via interspecies protoplast fusion, converting oxygen from an inhibitory stress into a programmable signal for sustained indole‐3‐propionic acid (IPA) production. This genetically encoded bioreactor enables robust aerobic operation and therapeutic efficacy of deoxynivalenol detoxification in vivo ...
Ning Ma, Jiakun Shen, Kun Cai, Wenxi Zhao, Chuan Feng, Xingyao Wang, Yangyang Liu, Dongyan Zhang, Xi Ma   +8 more
wiley   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Early protoplast culture and partial regeneration in Cannabis sativa: gene expression dynamics of proliferation and stress response

Frontiers in Plant Science
Cannabis sativa L. is a plant of significant interest due to its high content of biologically active compounds, durable fibers, and bioeconomic potential.
Daniel Král, Josef Baltazar Šenkyřík, Vladan Ondřej   +2 more
doaj   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, EarlyView.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Optimal Parameters for the Isolation of Carrot Mesophyll Protoplasts In Vitro

Овощи России
The isolation of protoplasts from diverse plant species is a widely employed technique. The purpose of this work is to develop an efficient system for isolating and purifying mesophyll protoplasts from Daucus carota.
N. Aljaramany, S. G. Monakhos
doaj   +1 more source

Light-dependent regulation of DEL1 is determined by the antagonistic action of E2Fb and E2Fc [PDF]

, 2011
Endoreduplication represents a variation on the cell cycle in which multiple rounds of DNA replication occur without subsequent chromosome separation and cytokinesis, thereby increasing the cellular DNA content.
Berckmans, Barbara, Bögre, Laszlo, De Veylder, Lieven, Lammens, Tim, Magyar, Zoltan, Van Den Daele, Hilde   +5 more
core   +5 more sources

OsFKBP20‐1b stabilizes OsUPF1 and OsUPF2 to promote the degradation of aberrant mRNAs during dehydration stress

Journal of Integrative Plant Biology, EarlyView.
The rice protein OsFKBP20‐1b protects key RNA‐surveillance factors from breakdown, so they can better eliminate defective messages. This RNA quality‐control boost reduces errors and helps plants survive drought, revealing a link between RNA control and drought tolerance.
Haemyeong Jung, Hyun Ji Park, Seung Hee Jo, Ki‐Hong Jung, Choonkyun Jung, Hye Sun Cho   +5 more
wiley   +1 more source

Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici [PDF]

, 2012
Three terrestrial plants are known to perform C 4 photosynthesis without the dual-cell system by partitioning two distinct types of chloroplasts in separate cytoplasmic compartments.
Shiu-Cheung Lung, Makoto Yanagisawa, Simon DX Chuong   +2 more
core   +2 more sources