Results 41 to 50 of about 21,306 (282)

A Method for Isolation of Protoplasts from Dermatophytes [PDF]

open access: yesMicrobiology, 1984
A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO4 has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell wall material on these protoplasts.
Srikantha, T, Rao, G Ramananda
openaire   +2 more sources

A Variable Polyglutamine Repeat Affects Subcellular Localization and Regulatory Activity of a Populus ANGUSTIFOLIA Protein. [PDF]

open access: yes, 2018
Polyglutamine (polyQ) stretches have been reported to occur in proteins across many organisms including animals, fungi and plants. Expansion of these repeats has attracted much attention due their associations with numerous human diseases including ...
Barry, Kerrie   +12 more
core   +1 more source

Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb

open access: yesJournal of Applied Sciences and Environmental Management, 2013
Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
I Mukhtar, R Bajwa, G Nasim
doaj   +2 more sources

Determinação de metodologia para oisolamento de protoplastos de tangerina Cleópatra (Citrus reshni Hort.) Methodology choice for protoplast isolation in Cleopatra mandarin (Citrus reshni Hort.)

open access: yesScientia Agricola, 1995
A hibridação somática via fusão de protoplastos vem sendo utilizada no melhoramento de porta-enxertos de citros em diversos países. Nos Estados Unidos, vários estudos demonstram a eficiência de procedimentos no isolamento e cultivo de protoplastos dessa ...
R.P. de Oliveira   +2 more
doaj   +1 more source

Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library [PDF]

open access: yes, 2013
BACKGROUND: The identification of endogenous cis-regulatory DNA elements (CREs) responsive to endogenous and environmental cues is important for studying gene regulation and for biotechnological applications but is labor and time intensive. Alternatively,
Ahmadinejad, N.   +3 more
core   +2 more sources

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

open access: yesFrontiers in Plant Science, 2023
Passion fruit (Passiflora edulis) is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values.
Linxi Wang   +8 more
doaj   +1 more source

Current advances in genomics and breeding of leaf chicory (Cichorium intybus L.) [PDF]

open access: yes, 2016
This review gives an overview of agricultural topics on a non-model species, in other words, leaf chicory. Often classified as a minor crop, \u201cRadicchio\u201d, the Italian name of leaf chicory, is assuming a very important role at both a local and ...
Barcaccia, Gianni   +2 more
core   +2 more sources

Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera

open access: yesPlant Methods, 2022
Background Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research.
Sufang Li   +8 more
doaj   +1 more source

High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata [PDF]

open access: yes, 2010
In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts.
Geelen, Danny, Lambert, Ellen
core   +1 more source

Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions

open access: yesAdvanced Science, EarlyView.
This review maps the fast‐evolving landscape of plant genetic engineering, linking enabling platforms with trait‐focused applications in architecture optimization, stress resilience, yield improvement, and quality enhancement. It highlights how genome editing, transgenic strategies, and emerging multi‐gene approaches reshape breeding pipelines, while ...
Peilin Wang   +4 more
wiley   +1 more source

Home - About - Disclaimer - Privacy