Results 41 to 50 of about 734,035 (251)

Protoplast Isolation - Incubation Buffer v1

, 2016
Instructions to make 50 mL of incubation buffer for protoplast isolation. Adapted from Yoo et al. 2007http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.html
Steven Burgess
core   +1 more source

A Method for Isolation of Protoplasts from Dermatophytes [PDF]

Microbiology, 1984
A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO4 has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell wall material on these protoplasts.
Srikantha, T, Rao, G Ramananda
openaire   +2 more sources

Plant cell protoplast isolation v1

, 2016
A protocol for isolation of protoplast from plant cells adapted from Yoo et al. 2007 (http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.htm). Procedure was optimized for use on Arabidopsis thaliana tissue.
Steven Burgess
core   +1 more source

Isolation and transformation of rice aleurone protoplasts [PDF]

Plant Cell Reports, 1994
Protoplasts isolated from the aleurone have been used extensively in molecular studies focusing on hormone-mediated regulation of gene expression in barley seed. To extend the use of aleurone protoplasts to other species, we have determined the conditions necessary for the isolation of protoplasts from rice aleurone layers of germinated seed.
S, Sadasivam, D R, Gallie
openaire   +3 more sources

The BTB/POZ‐MATH Protein ZmBPM1 Interacts With Autophagy‐related Protein ZmATG6 and Modulates the NLR Protein Rp1‐D21‐Mediated Defense Response in Maize

Advanced Science, EarlyView.
The maize BTB/POZ‐MATH (BPM) protein ZmBPM1 interacts with autophagy‐related protein ZmATG6 to relocate the NLR protein Rp1‐D21 from the nucleo‐cytoplasmic compartment into autophagosome‐like puncta, leading to autophagy‐mediated turnover and suppressing the hypersensitive response.
Chang‐Xiao Tang, Tong‐Tong Liu, Qi‐Dong Ge, Xiao‐Ying Liu, Wanxiao Wang, Yang Sun, Yanfei Liu, Faqiang Li, Guan‐Feng Wang   +8 more
wiley   +1 more source

OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES [PDF]

Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis.
Wael, Syahran, Mose, Windi
core   +1 more source

OsCPK12‐OsATPD1 Module Regulates Photosynthetic Acclimation to Light Stress in Rice

Advanced Science, EarlyView.
OsCPK12 interacts and phosphorylates OsAtpD1 at Ser8 in its transit peptide. This phosphorylation promote its chloroplast translocation, thereby maintaining ATP synthase activity and proton gradient (ΔpH) homeostasis and regulating photosynthetic acclimation, photoprotection, and repair under light stress.
Beifang Wang, Aaron Chan, Yongyi Fan, Yanwu Wang, Pao Xue, Yingxin Zhang, Xiaodeng Zhan, Weixun Wu, Ping Yu, Daibo Chen, Junlin Fu, Yongbo Hong, Xihong Shen, Lianping Sun, Aike Zhu, Shihua Cheng, Qunen Liu, Liyong Cao   +17 more
wiley   +1 more source

Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera

Plant Methods, 2022
Background Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research.
Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao, Deyi Yuan   +8 more
doaj   +1 more source

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

Frontiers in Plant Science, 2023
Passion fruit (Passiflora edulis) is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values.
Linxi Wang, Haobin Liu, Peilan Liu, Guanwei Wu, Wentao Shen, Hongguang Cui, Hongguang Cui, Zhaoji Dai, Zhaoji Dai   +8 more
doaj   +1 more source

The MoSR‐MoDde1 Regulatory Axis: A Novel Determinant of DMI Sensitivity and a Target for Sustainable Rice Blast Control

Advanced Science, EarlyView.
The transcription factor MoSR governs DMI fungicide sensitivity in Magnaporthe oryzae through dual regulation of ergosterol biosynthesis and the detoxification enzyme MoDde1. Targeting MoDde1 via a small‐molecule inhibitor or host‐induced gene silencing enhances DMI efficacy against rice blast, offering a sustainable disease management strategy ...
Fan‐Zhu Meng, Wen‐Kai Wei, Meng‐Yao Wu, Min‐Zheng Cai, Yu‐Fu Wang, Li Zhao, Shuai Meng, Liang‐Fen Yin, Guido Schnabel, Wei‐Xiao Yin, Chao‐Xi Luo   +10 more
wiley   +1 more source