Results 41 to 50 of about 698,061 (253)

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES [PDF]

Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis.
Wael, Syahran, Mose, Windi
core   +1 more source

Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera

Plant Methods, 2022
Background Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research.
Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao, Deyi Yuan   +8 more
doaj   +1 more source

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

Frontiers in Plant Science, 2023
Passion fruit (Passiflora edulis) is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values.
Linxi Wang, Haobin Liu, Peilan Liu, Guanwei Wu, Wentao Shen, Hongguang Cui, Hongguang Cui, Zhaoji Dai, Zhaoji Dai   +8 more
doaj   +1 more source

Single‐cell Transcriptome Profiling Reveals Gene Regulatory Networks and Key Genes in the Root Epidermis and Cortical Cells Associated with Early Nodulation in Glycine Max

Advanced Science, EarlyView.
Single‐cell transcriptomics of soybean roots soon after rhizobial inoculation reveals epidermal and cortical cell‐specific programs and gene‐regulatory networks acting in symbiosis establishment. We identify an ethylene‐driven regulatory circuit involving WRKY6.3/6.4 transcription factors targeting select Nod19 genes that promotes infection‐thread ...
Yongbin Zhuang, Yu Geng, Xinyu Guo, Chen Wang, Yongjing Sun, Luqi Liu, Li Liu, QianQian Gao, XinFang Zhu, Hongxiang Cao, Gengshuo Zhang, Yunxia Liu, Guobin Zhang, Baoyin Chen, Jinfei Zhang, Xin Hou, Xiaoming Li, Dajian Zhang   +17 more
wiley   +1 more source

SIZ1‐Mediated SUMOylation of LBD29 Recruits ARF7 to Fine‐Tune Auxin Signaling in Lateral Root Development

Advanced Science, EarlyView.
Our findings establish SUMOylation as a key post‐translational control layer in auxin signaling. We show that SIZ1‐mediated SUMOylation of LBD29 promotes its association with ARF7, stabilizing a transcriptional hub that governs lateral root development.
Jiaxuan Sui, Qianlan Yin, Yiying Chen, Liping Zhang, Xiuyuan Yu, Qianqian Yu, Zhaojun Ding, Xiangpei Kong   +7 more
wiley   +1 more source

Tomato: a crop species amenable to improvement by cellular and molecular methods [PDF]

, 1989
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
Zabel, Pim, Ramanna, M.S., Koornneef, Maarten, Koornneef, M., Hille, Jacques,, Hille, J., Zabel, P., Koornneef, Maarten,, Ramanna, M.S.,, Zabel, Pim,, Hille, Jacques   +10 more
core   +2 more sources

Protoplast Isolation and Regeneration from Gracilaria changii (Gracilariales, Rhodophyta)

, 2008
The tropical agarophyte Gracilaria changii has been much researched and documented by the Algae Research Laboratory, University of Malaya, especially with regards to its potential as a seaweed bioreactor for valuable compounds. Protoplast regeneration of
Yeong, Hui Yin, Khalid, Norzulaani, Phang, Siew Moi   +2 more
core   +2 more sources

Optimization of isolation and transfection conditions of maize endosperm protoplasts

Plant Methods, 2020
Background Endosperm-trait related genes are associated with grain yield or quality in maize. There are vast numbers of these genes whose functions and regulations are still unknown. The biolistic system, which is often used for transient gene expression,
Yufeng Hu, Dalin Song, Lei Gao, Babatope Samuel Ajayo, Yongbin Wang, Huanhuan Huang, Junjie Zhang, Hanmei Liu, Yinghong Liu, Guowu Yu, Yongjian Liu, Yangping Li, Yubi Huang   +12 more
doaj   +1 more source

The BTB/POZ‐MATH Protein ZmBPM1 Interacts With Autophagy‐related Protein ZmATG6 and Modulates the NLR Protein Rp1‐D21‐Mediated Defense Response in Maize

Advanced Science, EarlyView.
The maize BTB/POZ‐MATH (BPM) protein ZmBPM1 interacts with autophagy‐related protein ZmATG6 to relocate the NLR protein Rp1‐D21 from the nucleo‐cytoplasmic compartment into autophagosome‐like puncta, leading to autophagy‐mediated turnover and suppressing the hypersensitive response.
Chang‐Xiao Tang, Tong‐Tong Liu, Qi‐Dong Ge, Xiao‐Ying Liu, Wanxiao Wang, Yang Sun, Yanfei Liu, Faqiang Li, Guan‐Feng Wang   +8 more
wiley   +1 more source