Results 21 to 30 of about 734,035 (251)

Marchantia Protoplast Isolation v1

, 2016
Marchantia protoplast preparation (adapted from Bopp and Vicktor 1988)
Christian Boehm
core   +2 more sources

Virus Isolation and Rice Protoplast Infection

Bio-Protocol
Rice (Oryza sativa), a staple crop sustaining half of humanity’s caloric intake, is threatened by numerous insect-vector-transmitted diseases, such as rice stripe disease, caused by the rice stripe virus (RSV).
Yu Huang, Zhirui Yang, Yi Li
doaj   +3 more sources

Embryogenic Stem Cell Identity after Protoplast Isolation from Daucus carota and Recovery of Regeneration Ability through Protoplast Culture. [PDF]

Int J Mol Sci, 2022
Protoplasts are single cells isolated from tissues or organs and are considered a suitable system for cell studies in plants. Embryogenic cells are totipotent stem cells, but their regeneration ability decreases or becomes lost altogether with extension ...
Han JE, Lee HS, Lee H, Cho H, Park SY.
europepmc   +2 more sources

Solutions used for protoplast isolation and transfection.

, 2023
Solutions used for protoplast isolation and transfection.
Michael J. Thomson (8477751), Endang M. Septiningsih (6003947), Aya Bridgeland (15188045), Sudip Biswas (8506077), Nikolaos Tsakirpaloglou (15188048)   +4 more
core   +1 more source

Isolation of Chloroplasts from Plant Protoplasts [PDF]

Cold Spring Harbor Protocols, 2015
Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts.
Lung, SC, Chuong, SDX, Smith, MD
openaire   +4 more sources

A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]

Plant Methods, 2023
Background Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance.
Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi, Shiv Shanker Pandey   +4 more
doaj   +1 more source

Development of an efficient leaf protoplast isolation and transient expression system for Artemisia japonica

Biologia Plantarum, 2023
Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng, X. Fu, B. Peng, Q. Miao, S. Zeng, K. Tang, Q. Pan   +6 more
doaj   +1 more source

Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. [PDF]

PLoS ONE, 2012
BackgroundPopulus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.Methodology/principal findingsWe optimized a Populus leaf mesophyll
Jianjun Guo, Jennifer L Morrell-Falvey, Jessy L Labbé, Wellington Muchero, Udaya C Kalluri, Gerald A Tuskan, Jin-Gui Chen   +6 more
doaj   +1 more source

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato (Lycopersicon esculentum Mill.) protoplast yield

Acta Agrobotanica, 2013
The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was
Elżbieta Kuźniak, Marzena Wielanek, Urszula Małolepsza, Henryk Urbaniak   +3 more
doaj   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source