Results 21 to 30 of about 21,306 (282)

Development of an efficient leaf protoplast isolation and transient expression system for Artemisia japonica

Biologia Plantarum, 2023
Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng, X. Fu, B. Peng, Q. Miao, S. Zeng, K. Tang, Q. Pan   +6 more
doaj   +1 more source

Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. [PDF]

PLoS ONE, 2012
BackgroundPopulus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.Methodology/principal findingsWe optimized a Populus leaf mesophyll
Jianjun Guo, Jennifer L Morrell-Falvey, Jessy L Labbé, Wellington Muchero, Udaya C Kalluri, Gerald A Tuskan, Jin-Gui Chen   +6 more
doaj   +1 more source

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato (Lycopersicon esculentum Mill.) protoplast yield

Acta Agrobotanica, 2013
The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was
Elżbieta Kuźniak, Marzena Wielanek, Urszula Małolepsza, Henryk Urbaniak   +3 more
doaj   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source

Isolation of Chloroplasts from Plant Protoplasts [PDF]

Cold Spring Harbor Protocols, 2015
Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts.
Lung, SC, Chuong, SDX, Smith, MD
openaire   +4 more sources

Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase

Makara Journal of Science, 2014
The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production.
Wijanarka Wijanarka, Endang Sutariningsih Soetarto, Kumala Dewi, Ari Indrianto   +3 more
doaj   +1 more source

Asymmetric somatic plant hybridization : status and applications [PDF]

, 2013
To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product.
Deryckere, Dieter, Eeckhaut, Tom, Lakshmanan, Prabhu Shankar, Van Bockstaele, Erik, Van Huylenbroeck, Johan   +4 more
core   +2 more sources

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review

Horticultural Science, 2004
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-
B. Navrátilová
doaj   +1 more source

Tomato resistance to Alternaria stem canker: localization in host genotypes and functional expression compared to non-host resistance [PDF]

, 1989
The Alternaria stem canker resistance locus (Asc-locus), involved in resistance to the fungal pathogen Alternaria alternata f. sp. lycopersici and in insensitivity to host-specific toxins (AAL-toxins) produced by the pathogen, was genetically mapped on ...
Griend, E.G. van de,, Hille, J.,, Nijkamp, H.J.J.,, Tiersma, J.B.,, Witsenboer, H.M.A.,   +4 more
core   +2 more sources