Results 21 to 30 of about 698,061 (253)

Development of an efficient leaf protoplast isolation and transient expression system for Artemisia japonica

Biologia Plantarum, 2023
Artemisia japonica Thunb is an important perennial herb containing abundant chemical compounds utilized in conventional medicine for the treatment of malaria, hepatitis, hypertension, and inflammation.
B. Deng, X. Fu, B. Peng, Q. Miao, S. Zeng, K. Tang, Q. Pan   +6 more
doaj   +1 more source

Solutions used for protoplast isolation and transfection.

, 2023
Solutions used for protoplast isolation and transfection.
Michael J. Thomson (8477751), Endang M. Septiningsih (6003947), Aya Bridgeland (15188045), Sudip Biswas (8506077), Nikolaos Tsakirpaloglou (15188048)   +4 more
core   +1 more source

Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. [PDF]

PLoS ONE, 2012
BackgroundPopulus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.Methodology/principal findingsWe optimized a Populus leaf mesophyll
Jianjun Guo, Jennifer L Morrell-Falvey, Jessy L Labbé, Wellington Muchero, Udaya C Kalluri, Gerald A Tuskan, Jin-Gui Chen   +6 more
doaj   +1 more source

Effects of environmental preconditioning, donor tissue and isolation conditions on tomato (Lycopersicon esculentum Mill.) protoplast yield

Acta Agrobotanica, 2013
The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was
Elżbieta Kuźniak, Marzena Wielanek, Urszula Małolepsza, Henryk Urbaniak   +3 more
doaj   +1 more source

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Plant Methods, 2020
Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants.
Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho   +4 more
doaj   +1 more source

Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols in Eucommia ulmoides. [PDF]

Plants (Basel)
Eucommia ulmoides, a tree species native to China, holds considerable medicinal, ecological, and industrial importance. However, the absence of an efficient and stable genetic transformation system poses significant challenges to gene function studies ...
Zhou H, Zhou Z, Zhang J, Kan H, Yin M, Zhang H, Wang L, Zhao J, Ye J.   +8 more
europepmc   +2 more sources

Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase

Makara Journal of Science, 2014
The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production.
Wijanarka Wijanarka, Endang Sutariningsih Soetarto, Kumala Dewi, Ari Indrianto   +3 more
doaj   +1 more source

Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn

BMC Plant Biology, 2023
Background Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds.
Magdalena Zaranek, Reneé Pérez-Pérez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus   +4 more
doaj   +1 more source

Quick and efficient protoplast isolation from Neurospora crassa. [PDF]

, 1983
Quick and efficient protoplast isolation from Neurospora ...
F. M. Nemeti, Szabo, G., M. Schablik, Nemeti, F. M., G. Szabo, Schablik, M.   +5 more
core   +1 more source

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review

Horticultural Science, 2004
The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-
B. Navrátilová
doaj   +1 more source