Results 11 to 20 of about 21,306 (282)

Isolation and Transfection of Protoplasts From Maize Mesophyll Cells [PDF]

Bio-Protocol
Protoplast systems are widely used in plant research as versatile platforms for studying cellular processes and validating gene editing tools. In maize, they are particularly valuable because stable transformation in immature embryos is slow and labor ...
Lauren Higa, Taren Bouwman, Zhi-Yan Du
doaj   +2 more sources

Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing. [PDF]

PLoS ONE, 2017
Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts.
Xiaoyong Xu, Xiaoling Xu, Yipeng Zhou, Shaohua Zeng, Weiwen Kong   +4 more
doaj   +2 more sources

Protoplast Isolation in Lupin Lupinus spp

Journal of Agricultural Sciences, 1996
The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found ...
Necmi Beşer, Andrew Wetten
doaj   +3 more sources

An Efficient and Universal Protoplast Isolation Protocol Suitable for Transient Gene Expression Analysis and Single-Cell RNA Sequencing [PDF]

International Journal of Molecular Sciences, 2022
Juanjuan Wang, Yang Wang, Tian-Feng Lu   +2 more
exaly   +2 more sources

PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry [PDF]

Plant Methods
Background Black huckleberry (Vaccinium membranaceum) is a native fruit species of high nutritional, medicinal, ecological, and economic value. The black huckleberries, abundant in bioactive compounds, offer significant antioxidants and anti-inflammatory
Sweety Majumder, Abir U. Igamberdiev, Samir C. Debnath   +2 more
doaj   +2 more sources

Virus Isolation and Rice Protoplast Infection

Bio-Protocol
Rice (Oryza sativa), a staple crop sustaining half of humanity’s caloric intake, is threatened by numerous insect-vector-transmitted diseases, such as rice stripe disease, caused by the rice stripe virus (RSV).
Yu Huang, Zhirui Yang, Yi Li
doaj   +3 more sources

Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.

Agriculture, 2021
Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed, Miao Miao, Emmanouil D. Pratsinakis, Hongliang Zhang, Wei Wang, Yuan Yuan, Meiling Lyu, Junaid Iftikhar, Ahmed F. Yousef, Panagiotis Madesis, Binghua Wu   +10 more
doaj   +1 more source

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +11 more sources

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines

BMC Research Notes, 2023
Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela, Danna Susunaga-Gómez, Catalina Restrepo-Osorio, Carsten Harms, Diego Villanueva-Mejía   +4 more
doaj   +1 more source

A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]

Plant Methods, 2023
Background Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance.
Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi, Shiv Shanker Pandey   +4 more
doaj   +1 more source