Results 11 to 20 of about 698,061 (253)
Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture, 2021 Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed +10 more
doaj +3 more sources
Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing. [PDF]
PLoS ONE, 2017 Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts.
Xiaoyong Xu +4 more
doaj +2 more sources
Protoplast Isolation in Lupin Lupinus spp
Journal of Agricultural Sciences, 1996 The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found ...
Necmi Beşer, Andrew Wetten
doaj +3 more sources
Effect of Different Cellulase and Pectinase Enzyme Treatments on Protoplast Isolation and Viability in Lilium ledebeourii Bioss. [PDF]
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012 For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant withdesired traits. For this purpose, protoplast preparation is a first and important step.
Esmaeil CHAMANI +5 more
doaj +2 more sources
Embryogenic Stem Cell Identity after Protoplast Isolation from Daucus carota and Recovery of Regeneration Ability through Protoplast Culture. [PDF]
Int J Mol Sci, 2022 Protoplasts are single cells isolated from tissues or organs and are considered a suitable system for cell studies in plants. Embryogenic cells are totipotent stem cells, but their regeneration ability decreases or becomes lost altogether with extension ...
Han JE, Lee HS, Lee H, Cho H, Park SY.
europepmc +2 more sources
Virus Isolation and Rice Protoplast Infection
Bio-ProtocolRice (Oryza sativa), a staple crop sustaining half of humanity’s caloric intake, is threatened by numerous insect-vector-transmitted diseases, such as rice stripe disease, caused by the rice stripe virus (RSV).
Yu Huang, Zhirui Yang, Yi Li
doaj +3 more sources
Marchantia Protoplast Isolation v1
, 2016 Marchantia protoplast preparation (adapted from Bopp and Vicktor 1988)
Christian Boehm
openaire +2 more sources
BMC Research Notes, 2023 Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela +4 more
doaj +1 more source
Isolation of Chloroplasts from Plant Protoplasts [PDF]
Cold Spring Harbor Protocols, 2015 Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts.
Lung, SC, Chuong, SDX, Smith, MD
openaire +4 more sources
Plant Methods, 2023 Background Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance.
Abhishek Kumar +4 more
doaj +1 more source