Results 11 to 20 of about 734,035 (251)
Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing. [PDF]
PLoS ONE, 2017 Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts.
Xiaoyong Xu +4 more
doaj +3 more sources
Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture, 2021 Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and ...
Mohamed A. A. Ahmed +10 more
doaj +3 more sources
Protoplast Isolation and Transfection in
, 2022 Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars.
Boshu Li +5 more
core +4 more sources
Protoplast Isolation and Transformation in Oil
, 2022 187202The protocol outlined in this chapter describes a detailed procedure for protoplast isolation and transformation using polyethylene glycol (PEG)-mediated transfection and DNA microinjection, highlighting also the critical steps associated with the ...
Noll, Gundula +11 more
core +3 more sources
Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase [PDF]
Makara Journal of Science, 2014 The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production.
Wijanarka Wijanarka +3 more
doaj +2 more sources
Protoplast isolation and staining
, 2015 The successful isolation of mesophyll protoplasts from plant species has become a versatile tool for in vivo imaging of subcellular structures. Taking advantage of the various cytochemical probes available, the subcellular localization of specific ...
Lung, SC +13 more
core +4 more sources
BMC Research Notes, 2023 Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis.
Paula Figueroa-Varela +4 more
doaj +2 more sources
Effect of Different Cellulase and Pectinase Enzyme Treatments on Protoplast Isolation and Viability in Lilium ledebeourii Bioss. [PDF]
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012 For overcoming interspecific incompatibility, protoplast combination method is a proper procedure for making a new plant withdesired traits. For this purpose, protoplast preparation is a first and important step.
Esmaeil CHAMANI +5 more
doaj +2 more sources
Isolation of Mesophyll Protoplasts from Leaves of Dalbergia sissoo Roxb [PDF]
Journal of Applied Sciences and Environmental Management, 2013 Dalbergia sissoo is an important timber tree facing mysterious die back and wilting problem. In case of die back, Dalbergia is facing the threat of destruction in its natural habitats due to lack of potential pathogenicity test which is the major ...
I Mukhtar, R Bajwa, G Nasim
doaj +3 more sources
Protoplast Isolation in Lupin Lupinus spp
Journal of Agricultural Sciences, 1996 The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found ...
Necmi Beşer, Andrew Wetten
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