Results 11 to 20 of about 2,352,765 (289)

Transient Transformation of Plants

, 2009
Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins.
Jones, H. D., Doherty, A., Sparks, C. A.
openaire   +3 more sources

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies [PDF]

BMC Research Notes, 2020
Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector ...
Rita Sharma, Yan Liang, Mi Yeon Lee, Venkataramana R. Pidatala, Jenny C. Mortimer, Henrik V. Scheller   +5 more
doaj   +2 more sources

Transient Transformation of Artemisia annua

Bio-Protocol, 2015
Transient transformation of Artemisia annua does not depend on chromosomal integration of heterologous DNA, and recombinant DNA can be introduced into plant cells via Agrobacterium aided by vacuum.
Dongming Ma, Hong Wang
doaj   +2 more sources

Transient transformation of Podosphaera xanthii by electroporation of conidia. [PDF]

BMC Microbiol, 2015
Powdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact that has significantly limited their genetic manipulation.
Vela-Corcía D, Romero D, Torés JA, De Vicente A, Pérez-García A.   +4 more
europepmc   +5 more sources

On minimizing maximum transient energy growth [PDF]

, 2005
The problem of minimizing the maximum transient energy growth is considered. This problem has importance in some fluid flow control problems and other classes of non-linear systems.
Whidborne, James F., Steer, Anthony J., McKernan, John   +2 more
core   +7 more sources

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

Frontiers in Plant Science, 2023
The basic helix-loop-helix (bHLH) family is one of the most well-known transcription factor families in plants, and it regulates growth, development, and abiotic stress responses.
Quangang Liu, Quangang Liu, Jiaxing Wen, Jiaxing Wen, Shipeng Wang, Shipeng Wang, Jianhua Chen, Jianhua Chen, Yongqiang Sun, Yongqiang Sun, Qingbai Liu, Qingbai Liu, Xi Li, Xi Li, Shengjun Dong, Shengjun Dong   +15 more
doaj   +1 more source

The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use—A journey through a forest of regulatory and legal implications

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant expression using Agrobacterium-mediated transient transformation (ATT) of plants has developed into a robust and versatile method to rapidly produce proteins. The capability of plants to efficiently synthesize even homo- and hetero-multimeric
Holger Spiegel, Greta Nölke, Harry Thangaraj, Stefan Schillberg, Stefan Schillberg   +4 more
doaj   +1 more source

eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’

Plants, 2023
Although CRISPR/Cas-based genome editing has been widely used for plant genetic engineering, its application in the genetic improvement of trees has been limited, partly because of challenges in Agrobacterium-mediated transformation.
Guoliang Yuan, Yang Liu, Tao Yao, Wellington Muchero, Jin-Gui Chen, Gerald A. Tuskan, Xiaohan Yang   +6 more
doaj   +1 more source

Parameterisation of Transient Evoked Otoacoustic Emissions [PDF]

, 2004
In this paper we compare the application of the discrete wavelet transform (DWT) and wavelet packets (WP) to transient evoked otoacoustic emissions (TEOAE) to conduct a differential diagnosis of frequency-specifc hearing loss. By parameterising the TEOAE
Weiss, Stephan, Dietl, H., Weiss, S., Dietl, Hubert   +3 more
core   +3 more sources

A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar

Forestry Research, 2021
Determination of Cas9 cutting efficiency to the target sites is important for genome editing. However, this determination can only be made via an in vitro method, as the purification of Cas protein and synthesis of gRNA are necessary.
Zhibo Wang, Zihang He, Ming Qu, Zhujun Liu, Chao Wang, Yucheng Wang   +5 more
doaj   +1 more source