Results 11 to 20 of about 548,529 (284)
Transient Transformation of Artemisia annua
Bio-Protocol, 2015 Transient transformation of Artemisia annua does not depend on chromosomal integration of heterologous DNA, and recombinant DNA can be introduced into plant cells via Agrobacterium aided by vacuum.
Dongming Ma, Hong Wang
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A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus. [PDF]
PLoS ONE, 2015 Agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. However, the limitations of Agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants ...
Mitsuhiro Kimura +2 more
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Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols in Eucommia ulmoides [PDF]
PlantsEucommia ulmoides, a tree species native to China, holds considerable medicinal, ecological, and industrial importance. However, the absence of an efficient and stable genetic transformation system poses significant challenges to gene function studies ...
Hongrun Zhou +8 more
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Transient transformation of Podosphaera xanthii by electroporation of conidia. [PDF]
BMC Microbiol, 2015 Powdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact that has significantly limited their genetic manipulation.
Vela-Corcía D +4 more
europepmc +5 more sources
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant expression using Agrobacterium-mediated transient transformation (ATT) of plants has developed into a robust and versatile method to rapidly produce proteins. The capability of plants to efficiently synthesize even homo- and hetero-multimeric
Holger Spiegel +4 more
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Frontiers in Plant Science, 2023 The basic helix-loop-helix (bHLH) family is one of the most well-known transcription factor families in plants, and it regulates growth, development, and abiotic stress responses.
Quangang Liu +15 more
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Plants, 2023 Although CRISPR/Cas-based genome editing has been widely used for plant genetic engineering, its application in the genetic improvement of trees has been limited, partly because of challenges in Agrobacterium-mediated transformation.
Guoliang Yuan +6 more
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A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar
Forestry Research, 2021 Determination of Cas9 cutting efficiency to the target sites is important for genome editing. However, this determination can only be made via an in vitro method, as the purification of Cas protein and synthesis of gRNA are necessary.
Zhibo Wang +5 more
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National Science Open, 2023 CRISPR-based genome editing technologies continue to drive major advances in life sciences. A major challenge for realizing widespread use of genome editing in plants and agriculture is establishing methods that enable the rapid, comprehensive, and ...
Xing Sinian +6 more
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Unconventional critical exponents at dynamical quantum phase transitions in a random Ising chain [PDF]
, 2021 Dynamical quantum phase transitions (DQPTs) feature singular temporal behavior in transient quantum states during nonequilibrium real-time evolution.
Halimeh, Jad C. +2 more
core +3 more sources