Results 11 to 20 of about 5,895,543 (296)

Transient Transformation of Artemisia annua

open access: yesBio-Protocol, 2015
Transient transformation of Artemisia annua does not depend on chromosomal integration of heterologous DNA, and recombinant DNA can be introduced into plant cells via Agrobacterium aided by vacuum.
Dongming Ma, Hong Wang
doaj   +3 more sources

Transformation of Transient Overvoltages by Inductive Voltage Transformers [PDF]

open access: yesSensors, 2021
Overvoltage transients occur after any type of switching activity in a power network, such as breaker operation, fault occurrence/clearance and rapid load change. This distortion of voltage is transformed to the secondary circuit of a voltage transformer.
Michał Kaczmarek, Dariusz Brodecki
doaj   +4 more sources

Transient transformation of Podosphaera xanthii by electroporation of conidia [PDF]

open access: yesBMC Microbiology, 2015
BackgroundPowdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact
D. Vela-Corcía   +4 more
semanticscholar   +4 more sources

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

open access: yesFrontiers in Plant Science, 2023
The basic helix-loop-helix (bHLH) family is one of the most well-known transcription factor families in plants, and it regulates growth, development, and abiotic stress responses.
Quangang Liu   +15 more
doaj   +1 more source

The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use—A journey through a forest of regulatory and legal implications

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Recombinant expression using Agrobacterium-mediated transient transformation (ATT) of plants has developed into a robust and versatile method to rapidly produce proteins. The capability of plants to efficiently synthesize even homo- and hetero-multimeric
Holger Spiegel   +4 more
doaj   +1 more source

A Modified Method for Transient Transformation via Pollen Magnetofection in Lilium Germplasm

open access: yesInternational Journal of Molecular Sciences, 2023
Lily (Lilium spp.) is a popular ornamental plant. Traditional genetic transformation methods have low efficiency in lily, thus development of a high-efficiency genetic transformation system is important.
Ming-Fang Zhang   +9 more
semanticscholar   +1 more source

eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’

open access: yesPlants, 2023
Although CRISPR/Cas-based genome editing has been widely used for plant genetic engineering, its application in the genetic improvement of trees has been limited, partly because of challenges in Agrobacterium-mediated transformation.
Guoliang Yuan   +6 more
doaj   +1 more source

A method for determining the cutting efficiency of the CRISPR/Cas system in birch and poplar

open access: yesForestry Research, 2021
Determination of Cas9 cutting efficiency to the target sites is important for genome editing. However, this determination can only be made via an in vitro method, as the purification of Cas protein and synthesis of gRNA are necessary.
Zhibo Wang   +5 more
doaj   +1 more source

Establishment of a Transient Transformation Protocol in Cinnamomum camphora

open access: yesForests, 2023
Cinnamomum camphora is an excellent evergreen broad-leaved tree species with strong stress tolerance, but its molecular character revelation as well as ecological and economic value improvement were limited due to the lack of a genetic transformation ...
Siyi Zhang   +3 more
semanticscholar   +1 more source

Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality

open access: yesHorticulture Research, 2021
Although multiple microscopic techniques have been applied to horticultural research, few studies of individual organelles in living fruit cells have been reported to date.
Jinli Gong   +10 more
semanticscholar   +1 more source

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