Results 21 to 30 of about 5,895,543 (296)

A high-efficiency PEG-Ca2+-mediated transient transformation system for broccoli protoplasts

Frontiers in Plant Science, 2022
Transient transformation of plant protoplasts is an important method for studying gene function, subcellular localization and plant morphological development.
Dongxu Yang, Yong-Yu Zhao, Yu-Mei Liu, F. Han, Zhan-Sheng Li   +4 more
semanticscholar   +1 more source

An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar

BMC Plant Biology, 2021
Background Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology.
Lin Zheng, Jixiu Yang, Ya-Juan Chen, Li-Ping Ding, Jian-Hua Wei, Hong-Zhi Wang   +5 more
semanticscholar   +1 more source

Oxicam-type nonsteroidal anti-inflammatory drugs enhance Agrobacterium-mediated transient transformation in plants.

Plant Biotechnology, 2022
Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique.
Seung-Won Choi, Kie Kumaishi, R. Motohashi, H. Enoki, Wiluk Chacuttayapong, T. Takamizo, H. Saika, M. Endo, T. Yamada, Aya Hirose, N. Koizuka, S. Kimura, Yaichi Kawakatsu, Hiroyuki Koga, Emi Ito, K. Shirasu, Yasunori Ichihashi   +16 more
semanticscholar   +1 more source

Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species

National Science Open, 2023
CRISPR-based genome editing technologies continue to drive major advances in life sciences. A major challenge for realizing widespread use of genome editing in plants and agriculture is establishing methods that enable the rapid, comprehensive, and ...
Xing Sinian, Sun Yu, Li Boshu, Li Hongchao, Zhao Kevin T., Chen Kunling, Gao Caixia   +6 more
doaj   +1 more source

Agrobacterium-Mediated Transient Transformation of Marchantia Liverworts

bioRxiv, 2021
Agrobacterium-mediated transient gene expression is a rapid and useful approach for characterizing functions of gene products in planta. However, the practicability of the method in the model liverwort Marchantia polymorpha has not yet been thoroughly ...
Hidekazu Iwakawa, Katharina Melkonian, Titus Schlüter, Hyung-Woo Jeon, Ryuichi Nishihama, Hiroyasu Motose, H. Nakagami   +6 more
semanticscholar   +1 more source

An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays

Frontiers in Plant Science, 2022
Transient transformation in plants is a useful process for evaluating gene function. However, there is a scarcity of minimally perturbing methods for gene delivery that can be used on multiple organs, plant species, and non-excised tissues.
J. Morgan, J. Jeleńska, D. Hensley, S. Retterer, J. Morrell-Falvey, R. Standaert, J. Greenberg   +6 more
semanticscholar   +1 more source

An Easy and Rapid Transformation Protocol for Transient Expression in Cotton Fiber

Frontiers in Plant Science, 2022
Cotton fiber is the most important natural textile material in the world. Identification and functional characterization of genes regulating fiber development are fundamental for improving fiber quality and yield. However, stable cotton transformation is
Xiaoguang Shang, Lijie Zhu, Yujia Duan, Qingfei He, Meiyue Zhao, Yujia Yu, Wangzhen Guo   +6 more
doaj   +1 more source

Promoter cloning and activities analysis of JmLFY, a key gene for flowering in Juglans mandshurica

Frontiers in Plant Science, 2023
Juglans mandshurica (Manchurian walnut) is a precious timber and woody grain and oil species in Northeast China. The heterodichogamous characteristic phenomenon resulted in the non-synchronous flowering and development of male and female flowers, which ...
Lijie Zhang, Lijie Zhang, Jingqi Fu, Jingqi Fu, Tianyi Dong, Tianyi Dong, Mengmeng Zhang, Mengmeng Zhang, Jingwen Wu, Jingwen Wu, Chunping Liu, Chunping Liu, Chunping Liu   +12 more
doaj   +1 more source

The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea

Frontiers in Plant Science, 2018
Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete.
Xiangnan Meng, Xiangnan Meng, Yang Yu, Junyue Zhao, Na Cui, Tiefeng Song, Yun Yang, Haiyan Fan, Haiyan Fan   +8 more
doaj   +1 more source

Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions

Plants, 2020
A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and ...
Alexander A. Tyurin, Alexandra V. Suhorukova, Ksenia V. Kabardaeva, Irina V. Goldenkova-Pavlova   +3 more
doaj   +1 more source