TLM-Quant : an open-source pipeline for visualization and quantification of gene expression heterogeneity in growing microbial cells [PDF]
, 2013 Gene expression heterogeneity is a key driver for microbial adaptation to fluctuating environmental conditions, cell differentiation and the evolution of species. This phenomenon has therefore enormous implications, not only for life in general, but also
Piersma, Sjouke +25 more
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Functional modulation of the transient outward current Ito by KCNE beta-subunits and regional distribution in human non-failing and failing hearts [PDF]
, 2006 Objectives: The function of Kv4.3 (KCND3) channels, which underlie the transient outward current I,, in human heart, can be modulated by several accessory subunits such as KChIP2 and KCNE1-KCNE5.
Cotella, D. +8 more
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Transient light-activated gene expression in Chinese hamster ovary cells
BMC Biotechnology, 2021 Background Chinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and environmental approaches have been developed to modulate cellular pathways to improve titers. However, these methods
Shiaki A. Minami, Priya S. Shah
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The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription [PDF]
, 1986 To study trans-activation of gene expression by murine cytomegalovirus (MCMV) immediate-early (IE) proteins, the IE coding region 1 (ie1), which encodes the 89,000-Mr IE phosphoprotein (pp89), was stably introduced into L cells.
Koszinowski, Ulrich H. +5 more
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Construction of pGCGi, an expression vector carries intron containing GUS and analysis using micro-bombardment and agroinjection [PDF]
Zīst/shināsī-i Giyāhī-i Īrān, 2014 Transient gene expression is fast, easy and not influenced by positional effects that potentially affect on gene expression levels in stable gene transformation. Transient expression can be applicable using agroinfilteration, biolistic and viral vectors.
Mohammad Reza Zamani +2 more
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Frontiers in Microbiology, 2021 Gene silencing induced by hairpin RNA or virus infection expression is one of the major tools in genetics studies in plants. However, when dealing with essential genes, virus-induced gene silencing (VIGS) and transgenic expression of hairpin RNA could ...
Wang Zhang +6 more
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In vivo manipulation of interleukin-2 expression by a retroviral tetracycline (tet)-regulated system [PDF]
, 2006 We have used the tetracycline (tet)-regulated system as described previously to evaluate the applicability of controlled gene expression in cancer gene therapy. As a model gene, we used the human interleukin-2 (IL-2) gene, which has been placed under the
Wirth, Thomas +4 more
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Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes
BioTechTransient DNA transfection is routinely used to study gene function and elucidate the regulation of biological pathways, and it is also widely applied in biotechnology for large-scale recombinant protein production.
Shih-Yen Lo +5 more
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Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses [PDF]
, 2011 Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by ...
Wang, S.J. +9 more
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Fruit ResearchAgrobacterium-mediated transient gene expression is a powerful technique for rapidly evaluating gene expression in higher plants. This study aimed to improve transient expression levels of T-DNA genes in citrus by investigating the effects of various ...
Yanjun Li +6 more
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