Results 31 to 40 of about 773,655 (299)

Circumventing antivector immunity: potential use of nonhuman adenoviral vectors [PDF]

, 2014
Adenoviruses are efficient gene delivery vectors based on their ability to transduce a wide variety of cell types and drive high-level transient transgene expression. While there have been advances in modifying human adenoviral (HAdV) vectors to increase
Beard C.W., Benkö M., Cauthen A.N., Crawford-Miksza L., Estrella Lopez-Gordo, Gall J., Gall J.G., Gattoni A., Ginsberg H.S., Haviv Y.S., Hofmann C., Iva I. Podgorski, Kajon A.E., Kanerva A., Kass-Eisler A., Kuriyama S., Mastrangeli A., Michou A.I., Nicholas Downes, Ramon Alemany, Rogozhin V.N., Roy S., Seiradake E., Shmarov M.M., Soudais C., Tutykhina I.L., Wang D.Y., Wohlfart C., Ying B., Zakhartchouk A.N., Zeng Q.   +30 more
core   +3 more sources

Transient expression of chicken alpha interferon gene in lettuce [PDF]

Journal of Zhejiang University SCIENCE B, 2008
We investigated the possibility of producing chicken alpha interferon (ChIFN-alpha) in transgenic plants. The cDNA encoding ChIFN-alpha was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-alpha gene was correctly transcribed and translated in the lettuce plants according to RT-PCR ...
Li, Song, De-gang, Zhao, Yong-jun, Wu, Yi, Li   +3 more
openaire   +2 more sources

Development of a Viral RdRp-Assisted Gene Silencing System and Its Application in the Identification of Host Factors of Plant (+)RNA Virus

Frontiers in Microbiology, 2021
Gene silencing induced by hairpin RNA or virus infection expression is one of the major tools in genetics studies in plants. However, when dealing with essential genes, virus-induced gene silencing (VIGS) and transgenic expression of hairpin RNA could ...
Wang Zhang, Yanglin Qiu, Lingyun Zhou, Jinlong Yin, Liqun Wang, Haijian Zhi, Kai Xu   +6 more
doaj   +1 more source

miRNA-based rapid differentiation of purified neurons from hPSCs advancestowards quick screening for neuronal disease phenotypes in vitro [PDF]

, 2020
Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs).
Aoyama, Takeshi, Ishikawa, Mitsuru, Miyoshi, Hiroyuki, Morota, Saori, Nakamura, Mari, Okano, Hideyuki, Shibata, Shoichiro, Sone, Takefumi, Uchino, Hiroyuki, Watanabe, Hirotaka, Yoo, Andrew S   +10 more
core   +2 more sources

Transient light-activated gene expression in Chinese hamster ovary cells

BMC Biotechnology, 2021
Background Chinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and environmental approaches have been developed to modulate cellular pathways to improve titers. However, these methods
Shiaki A. Minami, Priya S. Shah
doaj   +1 more source

Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification [PDF]

, 2020
Non-invasive epigenome editing is a promising strategy for engineering gene expression programs, yet potency, specificity, and persistence remain challenging.
Acosta, Reyes, Baker, David, Bates, Daniel, Bloom, Jordan, Boyken, Scott E., Chen, Zibo, Ciarlo, Christie, Dunn, Douglass, Federation, Alexander, Funnell, Alister, Green, Shon, Heberlein, Gregory, Liao, Hanna, Nandakumar, Vivek, Otterman, Ericka, Pearl, Jocelynn, Queitsch, Konstantin, Sandstrom, Richard, Schanzer, Elaine, Stamatoyannopoulos, John A., Urnov, Fyodor D., Van Biber, Benjamin, Vong, Shinny, Wang, Hao, Wilken, Matthew S., Zrazhevskey, Pavel   +25 more
core  

Construction of pGCGi, an expression vector carries intron containing GUS and analysis using micro-bombardment and agroinjection [PDF]

Zīst/shināsī-i Giyāhī-i Īrān, 2014
Transient gene expression is fast, easy and not influenced by positional effects that potentially affect on gene expression levels in stable gene transformation. Transient expression can be applicable using agroinfilteration, biolistic and viral vectors.
Mohammad Reza Zamani, Farhad Shokouhifar, Mostafa Motallebi   +2 more
doaj  

Enhanced expression of tissue plasminogen activator via gene silencing suppressor strategy using P19 protein [PDF]

مجله بیوتکنولوژی کشاورزی, 2016
Human tissue-type plasminogen activator (tPA) is one of the most important therapeutically proteins involved in the breakdown of blood clots of brain and heart blood vessels following stroke.
Mahshid Amiri, Mokhtar Jalali Javaran, Parastou Ehsani, Rahim Haddad   +3 more
doaj   +1 more source

Co-expression networks in generation of induced pluripotent stem cells. [PDF]

, 2016
We developed an adenoviral vector, in which Yamanaka's four reprogramming factors (RFs) were controlled by individual CMV promoters in a single cassette (Ad-SOcMK). This permitted coordinated expression of RFs (SOX2, OCT3/4, c-MYC and KLF4) in a cell for
Coppola, Giovanni, Dansithong, Warunee, Figueroa, Karla P, Gao, Fuying, Paul, Sharan, Pflieger, Lance, Pulst, Stefan M   +6 more
core   +2 more sources

Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes

BioTech
Transient DNA transfection is routinely used to study gene function and elucidate the regulation of biological pathways, and it is also widely applied in biotechnology for large-scale recombinant protein production.
Shih-Yen Lo, Chee-Hing Yang, Yu-Ru Chan, Yi-Tzu Chao, Meng-Jiun Lai, Hui-Chun Li   +5 more
doaj   +1 more source