Results 11 to 20 of about 773,655 (299)
Transient Expression of cor Gene in Papaver somniferum
BioImpacts, 2011 Introduction: Papaver somniferum is the commercial source of morphine and codeine. The isolation of effective genes involved in the morphine biosynthesis of P.
Bahman Hosseini +6 more
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Transient phenomena in gene expression after induction of transcription. [PDF]
PLoS ONE, 2012 When transcription of a gene is induced by a stimulus, the number of its mRNA molecules changes with time. Here we discuss how this time evolution depends on the shape of the mRNA lifetime distribution.
Carlus Deneke +2 more
doaj +5 more sources
Quantitation of transient gene expression after electroporation [PDF]
Nucleic Acids Research, 1992 The combination of a photometric reporter-gene assay, with transfection by electroporation, is potentially a rapid and sensitive tool for the study of genetic regulatory elements in many types of cells. We have found that the sensitivity, accuracy, and reproducibility of the technique is greatly improved by the inclusion of appropriately chosen carrier
M K, Showe, D L, Williams, L C, Showe
openaire +2 more sources
Biolistic Approach for Transient Gene Expression Studies in Plants. [PDF]
Methods Mol Biol, 2020 Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research.
Lacroix B, Citovsky V.
europepmc +4 more sources
Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration [PDF]
Journal of Cell and Molecular Research, 2016 An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1.
Maziar Habibi-Pirkoohi +5 more
doaj +1 more source
Plants, 2021 Plant molecular farming has a great potential to produce valuable proteins. Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse
Yana Sindarovska, Mykola Kuchuk
doaj +1 more source
Different 3' end regions strongly influence the level of gene expression in plant cells [PDF]
, 1989 We have investigated the functional role of a 3' end region on the expression of a reporter gene in plant cells. In stably transformed plants, expression of the reporter gene without a plant gene 3' end is variable and depends on the fortuitous presence ...
Ingelbrecht, Ivan +4 more
core +1 more source
BMC Plant Biology, 2023 Background Safflower (Carthamus tinctorius L.) is an important economic crop and a traditional medicinal material rich in flavonoids, which can alleviate cardiovascular and cerebrovascular pathologies.
Bin Xian +5 more
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Local expression of expansin induces the entire process of leaf development and modifies leaf shape [PDF]
, 2001 Expansins are a family of extracellular proteins proposed to play a key role in wall stress relaxation and, thus, in cell and tissue growth. To test the possible function of expansins in morphogenesis, we have developed a technique that allows transient ...
Cho +14 more
core +2 more sources
Plant Methods, 2017 Background Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene ...
Guohui Yu +5 more
doaj +1 more source