Results 11 to 20 of about 5,757,325 (187)
Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]
Plant Methods, 2018 Background Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite
Karlah Norkunas +3 more
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Biolistic Approach for Transient Gene Expression Studies in Plants [PDF]
Methods Mol Biol, 2020 Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research.
Benoît, Lacroix, Vitaly, Citovsky
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Updated transient gene expression protocol in Expi293F cells using PEI [PDF]
Frontiers in Bioengineering and BiotechnologyTransient gene expression (TGE) is commonly used for the rapid production of protein-based therapeutics, including antibodies that require post-translational modifications.
Gustaf Hederoth +7 more
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Schema theory for gene expression programming [PDF]
, 2014 This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.This thesis studied a new variant of Evolutionary Algorithms called Gene Expression Programming.
Huang, Zhengwen
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Quantitation of transient gene expression after electroporation [PDF]
Nucleic Acids Research, 1992 The combination of a photometric reporter-gene assay, with transfection by electroporation, is potentially a rapid and sensitive tool for the study of genetic regulatory elements in many types of cells. We have found that the sensitivity, accuracy, and reproducibility of the technique is greatly improved by the inclusion of appropriately chosen carrier
M K, Showe, D L, Williams, L C, Showe
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Plant Methods, 2021 Background The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency.
Yongpeng Li +15 more
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Plants, 2021 Plant molecular farming has a great potential to produce valuable proteins. Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse
Yana Sindarovska, Mykola Kuchuk
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Virus-mediated transient expression techniques enable gene function studies in black-grass [PDF]
, 2020 Even though considerable progress has been made in weed ecology, weed molecular biology has been hindered by an inability to genetically manipulate weeds.
Macgregor, D. +4 more
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Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration [PDF]
Journal of Cell and Molecular Research, 2016 An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1.
Maziar Habibi-Pirkoohi +5 more
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Bio-Protocol, 2023 Virus-mediated transient gene overexpression and gene expression silencing can be used to screen gene functions in plants. Sugarcane mosaic virus (SCMV) is a positive strand RNA virus in the Potyviridae family that has been modified to be used as vector ...
Iram Gull, Georg Jander
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