Transient phenomena in gene expression after induction of transcription. [PDF]
PLoS ONE, 2012 When transcription of a gene is induced by a stimulus, the number of its mRNA molecules changes with time. Here we discuss how this time evolution depends on the shape of the mRNA lifetime distribution.
Carlus Deneke +2 more
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Optimization of transient gene expression in tomato (Solanum lycopersicum L.) [PDF]
مجله بیوتکنولوژی کشاورزی, 2023 ObjectiveThe aim of this study is to optimize tomato as a suitable plant system for transient gene transformation for the production of pharmaceutical and industrial compounds.Materials and methodsIn order to investigate transient gene expression, three ...
Masomeh Nasiri Mogadam +3 more
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Tightly regulated expression of Autographa californica multicapsid nucleopolyhedrovirus immediate early genes emerges from their interactions and possible collective behaviors. [PDF]
PLoS ONE, 2015 To infect their hosts, DNA viruses must successfully initiate the expression of viral genes that control subsequent viral gene expression and manipulate the host environment.
Chikako Ono +5 more
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TLM-Quant : an open-source pipeline for visualization and quantification of gene expression heterogeneity in growing microbial cells [PDF]
, 2013 Gene expression heterogeneity is a key driver for microbial adaptation to fluctuating environmental conditions, cell differentiation and the evolution of species. This phenomenon has therefore enormous implications, not only for life in general, but also
Piersma, Sjouke +25 more
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Gene Regulation in the Third Dimension [PDF]
, 2008 Analysis of the spatial organization of chromosomes reveals complex three-dimensional networks of chromosomal interactions. These interactions affect gene expression at multiple levels, including long-range control by distant enhancers and repressors ...
Dekker, Job, J. Dekker
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The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription [PDF]
, 1986 To study trans-activation of gene expression by murine cytomegalovirus (MCMV) immediate-early (IE) proteins, the IE coding region 1 (ie1), which encodes the 89,000-Mr IE phosphoprotein (pp89), was stably introduced into L cells.
Koszinowski, Ulrich H. +5 more
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Construction of pGCGi, an expression vector carries intron containing GUS and analysis using micro-bombardment and agroinjection [PDF]
Zīst/shināsī-i Giyāhī-i Īrān, 2014 Transient gene expression is fast, easy and not influenced by positional effects that potentially affect on gene expression levels in stable gene transformation. Transient expression can be applicable using agroinfilteration, biolistic and viral vectors.
Mohammad Reza Zamani +2 more
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Transient light-activated gene expression in Chinese hamster ovary cells
BMC Biotechnology, 2021 Background Chinese hamster ovary (CHO) cells are widely used for industrial production of biopharmaceuticals. Many genetic, chemical, and environmental approaches have been developed to modulate cellular pathways to improve titers. However, these methods
Shiaki A. Minami, Priya S. Shah
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Direct in vivo gene transfer to the airway epithelium employing adenovirus-polylysine-DNA complexes [PDF]
, 1993 Adenovirus-polylysine-DNA complexes were evaluated for their capacity to accomplish direct in vivo gene transfer to airway epithelium employing a rodent model.
Miller, L. +14 more
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Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes
BioTechTransient DNA transfection is routinely used to study gene function and elucidate the regulation of biological pathways, and it is also widely applied in biotechnology for large-scale recombinant protein production.
Shih-Yen Lo +5 more
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