Results 21 to 30 of about 5,757,325 (187)

Automation of large scale transient protein expression in mammalian cells [PDF]

, 2011
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Susan Daenke, Jones, M, E. Yvonne Jones, Siebold, Christian, Daenke, Susan, Christian Siebold, Bishop, Benjamin, Margaret Jones, Clay, Jordan E, Stuart, David I., A. Radu Aricescu, Zhao, Y, Benjamin Bishop, Aricescu, Alexandru R, Radu Aricescu, A, Stuart, David, Yvonne Jones, E, Weixian Lu, Stuart, David I, Clay, JE, Daenke, S, Aricescu, Alexandru, Bishop, B, Jones, EY, Clay, Jordan E., Yuguang Zhao, Jones, Margaret, Lu, Weixian, David I. Stuart, Radu Aricescu, A., Yvonne Jones, E., Aricescu, AR, Jones, Edith, Jordan E. Clay, Zhao, Yuguang, Jones, E Yvonne, Lu, W, Siebold, C, Stuart, DI   +38 more
core   +1 more source

A systems biology design and implementation of novel bioinformatics software tools for high throughput gene expression analysis [PDF]

, 2009
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Microarray technology has revolutionized the field of molecular biology by offering an efficient and cost effective platform for the simultaneous ...
Khan, Mohsin Amir Faiz
core   +7 more sources

The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L

BMC Plant Biology, 2023
Background Safflower (Carthamus tinctorius L.) is an important economic crop and a traditional medicinal material rich in flavonoids, which can alleviate cardiovascular and cerebrovascular pathologies.
Bin Xian, Ziqing Xi, Chaoxiang Ren, Jie Yan, Jiang Chen, Jin Pei   +5 more
doaj   +1 more source

Edge-weighting of gene expression graphs [PDF]

, 2009
In recent years, considerable research efforts have been directed to micro-array technologies and their role in providing simultaneous information on expression profiles for thousands of genes.
Kerr, Gráinne, Crane, Martin, DIMITRI PERRIN, HEATHER J. RUSKIN, Ruskin, Heather J., Perrin, Dimitri, MARTIN CRANE, GRAINNE KERR, Ruskin, Heather J   +8 more
core   +3 more sources

An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1

Plant Methods, 2017
Background Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene ...
Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang, Bingyu Zhao   +5 more
doaj   +1 more source

Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

Journal of Integrative Agriculture, 2017
Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly.
Fang LI, Su-ming DAI, Zi-niu DENG, Da-zhi LI, Gui-you LONG, Na LI, Yi LI, Alexandra Gentile   +7 more
doaj   +1 more source

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice [PDF]

Journal of Visualized Experiments, 2014
Efficient expression of transgenes in vivo is of critical importance in studying gene function and developing treatments for diseases. Over the past years, hydrodynamic gene delivery (HGD) has emerged as a simple, fast, safe and effective method for delivering transgenes into rodents.
Daniella, Kovacsics, Jayne, Raper
openaire   +2 more sources

Tightly regulated expression of Autographa californica multicapsid nucleopolyhedrovirus immediate early genes emerges from their interactions and possible collective behaviors. [PDF]

PLoS ONE, 2015
To infect their hosts, DNA viruses must successfully initiate the expression of viral genes that control subsequent viral gene expression and manipulate the host environment.
Chikako Ono, Masanao Sato, Hitomi Taka, Shin-ichiro Asano, Yoshiharu Matsuura, Hisanori Bando   +5 more
doaj   +1 more source

Optimization of transient gene expression in tomato (Solanum lycopersicum L.) [PDF]

مجله بیوتکنولوژی کشاورزی, 2023
ObjectiveThe aim of this study is to optimize tomato as a suitable plant system for transient gene transformation for the production of pharmaceutical and industrial compounds.Materials and methodsIn order to investigate transient gene expression, three ...
Masomeh Nasiri Mogadam, Asad Maroufi, Mokhtar Jalali Javaran, Yousef Sharafi   +3 more
doaj   +1 more source

Screening for coping style increases the power of gene expression studies [PDF]

, 2009
<b>Background:</b> Individuals of many vertebrate species show different stress coping styles and these have a striking influence on how gene expression shifts in response to a variety of challenges.
Pilarczyk, Maciej, Kadri, S., Davinia Morera Capdevila, Felicity A, Capdevila, Davinia Morera, Laia Ribas, Felicity A Huntingford, Huntingford, Felicity A, Simon MacKenzie, Ribas, L., MacKenzie, Simon, Sunil Kadri, Morera Capdevila, Davinia, Ribas, Laia, Pilarczyk, M., Morera, Davinia, MacKenzie, S., Huntingford, F.A., Maciej Pilarczyk, Kadri, Sunil, Capdevila, D.M., Huntingford, Felicity A., Ribas Cabezas, Laia   +22 more
core   +2 more sources