The human adenovirus 5 L4 promoter is activated by cellular stress response protein p53 [PDF]
During adenovirus infection, the emphasis of gene expression switches from early genes to late genes in a highly regulated manner. Two gene products, L4-22K and L4-33K, contribute to this switch by activating the Major Late Transcription Unit (MLTU) and ...
Leppard, Keith, Wright, Jordan
core +1 more source
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice [PDF]
Efficient expression of transgenes in vivo is of critical importance in studying gene function and developing treatments for diseases. Over the past years, hydrodynamic gene delivery (HGD) has emerged as a simple, fast, safe and effective method for delivering transgenes into rodents.
Daniella, Kovacsics, Jayne, Raper
openaire +2 more sources
Enhancement of Recombinant Protein Production in Transgenic Nicotiana benthamiana Plant Cell Suspension Cultures with Co-Cultivation of Agrobacterium Containing Silencing Suppressors. [PDF]
We have previously demonstrated that the inducible plant viral vector (CMViva) in transgenic plant cell cultures can significantly improve the productivity of extracellular functional recombinant human alpha-1-antiryspin (rAAT) compared with either a ...
Dandekar, Abhaya M +3 more
core +6 more sources
Tightly regulated expression of Autographa californica multicapsid nucleopolyhedrovirus immediate early genes emerges from their interactions and possible collective behaviors. [PDF]
To infect their hosts, DNA viruses must successfully initiate the expression of viral genes that control subsequent viral gene expression and manipulate the host environment.
Chikako Ono +5 more
doaj +1 more source
Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus
Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly.
Fang LI +7 more
doaj +1 more source
Kinetochore inactivation by expression of a repressive mRNA. [PDF]
Differentiation programs such as meiosis depend on extensive gene regulation to mediate cellular morphogenesis. Meiosis requires transient removal of the outer kinetochore, the complex that connects microtubules to chromosomes.
Chen, Jingxun +8 more
core +2 more sources
Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
core +3 more sources
Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing. [PDF]
RNA interference (RNAi) has emerged as a powerful way of reducing gene function in Drosophila melanogaster tissues. By expressing synthetic short hairpin RNAs (shRNAs) using the Gal4/UAS system, knockdown is efficiently achieved in specific tissues or in
Bosch, Justin A +2 more
core +1 more source
Transient Gene Expression in Intact and Organized Rice Tissues [PDF]
Regulated gene expression of chimeric genes has been studied extensively in electroporated protoplasts. The applicability of these assays is limited, however, because protoplasts are not always physiologically identical to the cells from which they are derived. We have developed a procedure to electroporate DNA into intact and organized leaf structures
R. A., Dekeyser +5 more
openaire +2 more sources
Optimization of transient gene expression in tomato (Solanum lycopersicum L.) [PDF]
ObjectiveThe aim of this study is to optimize tomato as a suitable plant system for transient gene transformation for the production of pharmaceutical and industrial compounds.Materials and methodsIn order to investigate transient gene expression, three ...
Masomeh Nasiri Mogadam +3 more
doaj +1 more source

