Primary Neuronal Culture and Transient Transfection [PDF]
Bio-ProtocolPrimary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro.
Shun- Tseng, Peng- Chen, Eric Hwang
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The Prodigious Potential of mRNA Electrotransfer as a Substitute to Conventional DNA-Based Transient Transfection [PDF]
Cells, 2023 Transient transfection of foreign DNA is the most widely used laboratory technique to study gene function and product. However, the transfection efficiency depends on many parameters, including DNA quantity and quality, transfection methods and target ...
Théo Juncker +2 more
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Microfluidic Transfection System and Temperature Strongly Influence the Efficiency of Transient Transfection [PDF]
ACS OmegaFor the process of transient transfection (TTF), DNA is often transported into the cells using polyplexes. The polyplex uptake and the subsequent transient expression of the gene of interest are of great importance for a successful transfection. In this study, we investigated a 3D-printed microfluidic system designed to facilitate direct TTF for ...
Michaela Dehne +7 more
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Transient Transfection of the Zoonotic Parasite Babesia microti [PDF]
Pathogens, 2020 The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection
Mingming Liu +11 more
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Development of novel lipoplex formulation methodologies to improve large-scale transient transfection for lentiviral vector manufacture [PDF]
Molecular Therapy: Methods & Clinical DevelopmentLarge-scale transient transfection has advanced significantly over the last 20 years, enabling the effective production of a diverse range of biopharmaceutical products, including viral vectors.
Thomas Williams-Fegredo +5 more
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Transient Transfection-based Fusion Assay for Viral Proteins [PDF]
Bio-Protocol, 2017 Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal set of ...
Melina Vallbracht +3 more
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Micro-dystrophin AAV Vectors Made by Transient Transfection and Herpesvirus System Are Equally Potent in Treating mdx Mouse Muscle Disease [PDF]
Molecular Therapy: Methods & Clinical Development, 2020 Vector production scale-up is a major barrier in systemic adeno-associated virus (AAV) gene therapy. Many scalable manufacturing methods have been developed.
Chady H. Hakim +13 more
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On Measuring miRNAs after Transient Transfection of Mimics or Antisense Inhibitors [PDF]
PLoS ONE, 2013 The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To achieve this there is widespread use of both exogenous double-stranded miRNA mimics for transient over-expression, and single stranded antisense RNAs (antimiRs) for miRNA inhibition.
Cameron P Bracken, Gregory J Goodall
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Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection. [PDF]
PLoS Computational Biology, 2020 The mapping of molecular inputs to their molecular outputs (input/output, I/O mapping) is an important characteristic of gene circuits, both natural and synthetic. Experimental determination of such mappings for synthetic circuits is best performed using
Christoph Stelzer, Yaakov Benenson
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A Transient Transfection-based Cell Adhesion Assay with 293T Cells [PDF]
Bio-Protocol, 2021 The in vitro cell adhesion assay is a quantitative method for measuring selective cell adhesion to specific proteins. Traditionally, cell adhesion assays employ purified protein immobilized on a solid glass or plastic surface.
Rohit Singh, Beom Choi
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